FLT3 Mutational Analysis, PCR
Detection of activating mutations in the receptor tyrosine kinase FLT3 that occur in 15% of acute myeloid leukemia. The mutations include an internal tandem duplication (ITD) in the juxtamembrane domain coding sequence and a missense mutation in the kinase domain (D835) of the FLT3 gene that impart a poor prognosis.
To select AML cases that are appropriate for targeted therapy.
A fluorescent-based PCR is used to detect ITD and D835 point mutations in Flt3 using DNA isolated from bone marrow or peripheral blood. Flt3/ITD is assessed by amplification of the JM domain using primers from exons 14 and 15. Mutations at D835 are assessed by restriction fragment length polymorphism mediated assay using primers flanking the mutation site.
This assay will detect internal tandem duplications (ITD) in the juxtamembrane domain and point mutations in codons 835 and 836. The sensitivity of the test is approximately one cell with mutated FLT3 in 20-100 total cells (1-5%).
Four to six working days
- 10 ml peripheral blood in purple top tube (EDTA Vacutainer), sent on wet ice
- 2-5 ml of bone marrow aspirate, sent on wet ice
Note: This test is only available for patients seen by physicians at MD Anderson Cancer Center.
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