Technologies and Platforms
The BD FACSMelody Cell Sorter
Echo Liquid Handler
10X Chromium Controller
- MiSeq (Illumina)
- NextSeq500 (Illumina)
- Hiseq4000 (Illumina)
- NovoSeq6000 (Illumina)
- Nextseq2000 (Illumina)
Sequencing services are performed through the Advanced Technology Genomics Core (ATGC) facility directed by Erika Thomson and data is returned to SINGLE CORE.
Data Analysis Support
- scRNA-seq analysis: clustering, high dimensional analysis, cell type identification, differential expression analysis, pseudotime inference, signature and pathway analysis
- scATAC-seq analysis: peak calling, DNA motif and transcription factor identification, pathway analysis
- scDNA-seq analysis: mutation calling, copy number profile inference, clustering, phylogenetic analysis, annotation of cancer genes and pathways
Users will meet with the computational scientists at SINGLE CORE for a consultation to discuss plans for the analysis of their datasets.