Services
Tissue Dissociation Services
- Dissociation of Fresh or Frozen Tissues into Nuclear Suspensions
- Dissociation of Fresh tissues into viable cell suspensions
- Cryostorage of viable cell suspensions
Next-Generation Sequencing
- MiSeq (Illumina)
- NextSeq500 (Illumina)
- Hiseq4000 (Illumina)
- NovoSeq6000 (Illumina)
Sequencing services are performed through the Advanced Technology Genomics Core (ATGC) facility directed by Erika Thomson and data is returned to SINGLE CORE.
Techonologies and Platforms
The BD FACSMelody Cell Sorter
Echo Liquid Handler
MissionBio Tapestri
10X Chromium Controller
Data Analysis Support
scRNA-seq analysis: clustering, high dimensional analysis, cell type identification, differential expression analysis, pseudotime inference, signature and pathway analysis
scATAC-seq analysis: peak calling, DNA motif and transcription factor identification, pathway analysis
scDNA-seq analysis: mutation calling, copy number profile inference, clustering, phylogenetic analysis, annotation of cancer genes and pathways
Users will meet with the computational scientists at SINGLE CORE for a consultation to discuss plans for the analysis of their datasets.