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Antibody Lists

Overview

Previous Antibody Lists contained incorrect or uncommon gene names that caused problems during RPPA data analysis. View the list of Updated Gene Names in the current antibody list for the latest information. If you need Previous Antibody Lists, or if you need the lists below in a different, more usable format, please contact us.

Meaning of Validation Status

Valid = RPPA and WB correlation > 0.7
Use with Caution = RPPA and WB correlation < 0.7
Under Evaluation = Antibody has given mixed results and/or evaluated by another lab; we are in the process of (re)validating
Used for QC = These antibodies are used for tissue sample quality control (QC); WILL NOT report to Datasets containing tissue samples, but WILL report to Datasets containing cell line samples

Expanded Antibody List: Set 209 to Current

#	RPPA Core Ab Name**	Ab Name Reported on Dataset	Gene Name	NCBI Entrez Gene ID	Company	Catalog no. (click on link)	Internal Ab ID	RRID	Species	Validation Status*	RPPA Dilution	Storage	UniProt no. (click on link)
1	14-3-3 zeta [discontinued]	14-3-3-zeta	YWHAZ	7534	Santa Cruz	sc-1019	883	AB_2218378	Rabbit	Valid	1:5000	4	P63104
2	4E-BP1	4E-BP1	EIF4EBP1	1978	CST	9452	2	AB_331692	Rabbit	Valid	1:100	-20	Q13541
3	4E-BP1 (phospho Ser65)	4E-BP1_pS65	EIF4EBP1	1978	CST	9456	3	AB_823413	Rabbit	Valid	1:250	-20	Q13541
4	4E-BP1 (phospho Thr37 Thr46)	4E-BP1_pT37_T46	EIF4EBP1	1978	CST	9459	6	AB_330985	Rabbit	Valid	1:3000	-20	Q13541
5	53BP1	53BP1	TP53BP1	7158	CST	4937	985	AB_10694558	Rabbit	Valid	1:300	-20	Q12888
6	AceCS1	AceCS1	ACSS2	55902	CST	3658	2372	AB_2222710	Rabbit	Valid	1:3000	-20	Q9NR19
7	Acetyl-CoA Carboxylase	ACC1	ACACA	31	Epitomics/Abcam	1768-1/ab45174	14	AB_867475	Rabbit	Caution	1:3000	-20	Q13085
7	Acetyl-CoA Carboxylase	ACC1	ACACB	32	Epitomics/Abcam	1768-1/ab45174	14	AB_867475	Rabbit	Caution	1:3000	-20	O00763
8	Acetyl-CoA Carboxylase (phospho Ser79)	ACC_pS79	ACACA	31	CST	3661	13	AB_330337	Rabbit	Valid	1:500	-20	Q13085
8	Acetyl-CoA Carboxylase (phospho Ser79)	ACC_pS79	ACACB	32	CST	3661	13	AB_330337	Rabbit	Valid	1:500	-20	O00763
9	ACSL1	ACSL1	ACSL1	2180	CST	9189	2367	AB_10891616	Rabbit	Valid	1:500	-20	P33121
10	ADAR1 [discontinued]	ADAR1	ADAR	103	Abcam	ab88574	1198	AB_2222658	Mouse	Valid	1:100	-20	P55265
11	Akt	Akt	AKT1	207	CST	4691	1084	AB_915783	Rabbit	Valid	1:7500	-20	P31749
			AKT2	208									P31751
			AKT3	10000									Q9Y243
12	Akt (phospho Ser473)	Akt_pS473	AKT1	207	CST	9271	230	AB_329825	Rabbit	Valid	1:150	-20	P31749
			AKT2	208									P31751
			AKT3	10000									Q9Y243
13	Akt (phospho Thr308)	Akt_pT308	AKT1	207	CST	2965	1154	AB_2255933	Rabbit	Valid	1:250	-20	P31749
			AKT2	208									P31751
			AKT3	10000									Q9Y243
14	Akt1	Akt1	AKT1	207	CST	2938	2010	AB_915788	Rabbit	Valid	1:1000	-20	P31749
15	Akt1 (phospho Ser473)	Akt1_pS473	AKT1	207	CST	9018	2008	AB_2629283	Rabbit	Valid	1:500	-20	P31749
16	Akt2	Akt2	AKT2	208	CST	3063	1800	AB_2225186	Rabbit	Valid	1:3000	-20	P31751
17	Akt2 (phospho Ser474)	Akt2_pS474	AKT2	208	CST	8599	2009	AB_2630347	Rabbit	Caution	1:400	-20	P31751
18	ALKBH5	ALKBH5	ALKBH5	54890	Abcam	ab195377	2577	AB_2827986	Rabbit	Valid	1:3000	-20	Q6P6C2
19	alpha-Tubulin (detyrosinated)	D-a-Tubulin	TUBA3C	7278	Abcam	ab48389	1379	AB_869990	Rabbit	Valid	1:1500	-20	P68366
19	alpha-Tubulin (detyrosinated)	D-a-Tubulin	TUBA4A	7277	Abcam	ab48389	1379	AB_869990	Rabbit	Valid	1:1500	-20	P68366
20	Ambra1 (phospho Ser52)	Ambra1_pS52	AMBRA1	55626	Millipore	ABC80	1630	AB_2750901	Rabbit	Caution	1:150	4	Q9C0C7
21	AMPK alpha	AMPKa	PRKAA1	5562	CST	2532	39	AB_330331	Rabbit	Caution	1:75	-20	Q13131
21	AMPK alpha	AMPKa	PRKAA2	5563	CST	2532	39	AB_330331	Rabbit	Caution	1:75	-20	P54646
22	AMPK alpha (phospho Thr172)	AMPKa_pT172	PRKAA1	5562	CST	2535	40	AB_331250	Rabbit	Caution	1:100	-20	Q13131
22	AMPK alpha (phospho Thr172)	AMPKa_pT172	PRKAA2	5563	CST	2535	40	AB_331250	Rabbit	Caution	1:100	-20	P54646
23	Androgen Receptor	AR	AR	367	CST	5153	2191	AB_10691711	Rabbit	Valid	1:100	-20	P10275
24	Annexin I	Annexin-I	ANXA1	301	BD Biosciences	610066	1208	AB_397477	Mouse	Valid	1:750	-20	P04083
25	A-Raf	A-Raf	ARAF	369	CST	4432	1217	AB_330813	Rabbit	Valid	1:300	-20	P10398
26	A-Raf (phospho Ser299)	A-Raf_pS299	ARAF	369	MyBioSource	MBS9614133	2686	AGID02318	Rabbit	Caution	1:500	-20	P10398
27	ARID1A	ARID1A	ARID1A	8289	Sigma-Aldrich	HPA005456	1442	AB_1078205	Rabbit	Caution	1:1000	-20	O14497
28	ASNS	ASNS	ASNS	440	Sigma-Aldrich	HPA029318	1560	AB_10602389	Rabbit	Valid	1:500	-20	P08243
29	Atg4B	Atg4B	ATG4B	23192	CST	13507	1775	AB_2750642	Rabbit	Caution	1:200	-20	Q9Y4P1
30	Atg5	Atg5	ATG5	9474	CST	12994	1776	AB_2630393	Rabbit	Caution	1:1000	-20	Q9H1Y0
31	Atg7	Atg7	ATG7	10533	CST	8558	1613	AB_10831194	Rabbit	Valid	1:250	-20	O95352
32	ATM	ATM	ATM	472	CST	2873	1363	AB_2062659	Rabbit	Valid	1:250	-20	Q13315
33	ATM (phospho Ser1981)	ATM_pS1981	ATM	472	CST	13050	2447	AB_2798100	Rabbit	Valid	1:20	-20	Q13315
34	ATP5A	ATP5A	ATP5F1A	498	Abcam	ab14748	1289	AB_301447	Mouse	Caution	1:50	4	P25705
35	ATP5H	ATP5H	ATP5PD	10476	Invitrogen	PA5-43776	2451	AB_2610004	Rabbit	Valid	1:200	-20	O75947
36	ATP-Citrate Lyase (phospho Ser455)	ACLY_pS455	ACLY	47	CST	4331	2368	AB_2257987	Rabbit	Valid	1:100	-20	P53396
37	ATR	ATR	ATR	545	CST	13934	2456	AB_2798347	Rabbit	Valid	1:250	-20	Q13535
38	ATR (phospho Ser428)	ATR_pS428	ATR	545	Abcam	ab178407	1795	AB_2721899	Rabbit	Caution	1:500	-20	Q13535
39	ATRX	ATRX	ATRX	546	Abcam	ab97508	1569	AB_10680289	Rabbit	Caution	1:1000	-20	P46100
40	Aurora A	Aurora-A	AURKA	6790	CST	14475	2332	AB_2665504	Rabbit	Caution	1:50	-20	O14965
41	Aurora A-C (phospho Thr288 Thr232 Thr198)	Aurora-ABC_pT288_pT232_pT198	AURKA	6790	CST	2914	2333	AB_2061631	Rabbit	Caution	1:100	-20	O14965
			AURKB	9212									Q96GD4
			AURKC	6795									Q9UQB9
42	Aurora B	Aurora-B	AURKB	9212	Invitrogen	MA5-27890	2512	AB_2744944	Rabbit	Valid	1:1500	-20	Q96GD4
43	Axl	Axl	AXL	558	CST	8661	1271	AB_11217435	Rabbit	Valid	1:500	-20	P30530
44	B7-H3	B7-H3	CD276	80381	CST	14058	1725	AB_2750877	Rabbit	Caution	1:150	-20	Q5ZPR3
45	B7-H4	B7-H4	VTCN1	79679	CST	14572	1726	AB_2750878	Rabbit	Caution	1:30	-20	Q7Z7D3
46	Bad (phospho Ser112)	Bad_pS112	BAD	572	CST	9291	63	AB_331417	Rabbit	Valid	1:50	-20	Q92934
47	Bak [special order]	Bak	BAK1	578	Epitomics/Abcam	1542-1/ab32371	71	AB_725624	Rabbit	Caution	1:400	-20	Q16611
48	BAP1	BAP1	BAP1	8314	Santa Cruz	sc-28383	1207	AB_626723	Mouse	Valid	1:50	4	Q92560
49	Bax	Bax	BAX	581	CST	2772	73	AB_10695870	Rabbit	Valid	1:100	-20	Q07812
50	Bcl-2	Bcl2	BCL2	596	CST	3498	2414	AB_725644	Rabbit	Caution	1:50	-20	P10415
51	Bcl2A1	Bcl2A1	BCL2A1	597	Abnova	PAB8528	1299	AB_1672563	Rabbit	Valid	1:250	-20	Q16548
52	Bcl-xL	Bcl-xL	BCL2L1	598	CST	2762	85	AB_10694844	Rabbit	Valid	1:100	-20	Q07817
53	Beclin 1	Beclin	BECN1	8678	ThermoFisher	PA1-16857	2269	AB_568459	Rabbit	Caution	1:500	4	Q14457
54	beta-Actin	b-Actin	ACTB	60	CST	8457	2449	AB_10950489	Rabbit	Caution	1:75	-20	P60709
55	beta-Catenin	b-Catenin	CTNNB1	1499	CST	9562	75	AB_331149	Rabbit	Valid	1:1500	-20	P35222
56	beta-Catenin (phospho Thr41 Ser45)	b-Catenin_pT41_S45	CTNNB1	1499	CST	9565	1170	AB_331731	Rabbit	Valid	1:20	-20	P35222
57	Bid	Bid	BID	637	CST	2002	89	AB_10692485	Rabbit	Caution	1:500	-20	P55957
58	Bim	Bim	BCL2L11	10018	CST	2933	1171	AB_1030947	Rabbit	Valid	1:500	-20	O43521
59	B-Raf	B-Raf	BRAF	673	CST	14814	2083	AB_2750887	Rabbit	Caution	1:500	-20	P15056
60	B-Raf (phospho Ser445)	B-Raf_pS445	BRAF	673	CST	2696	94	AB_390721	Rabbit	Valid	1:75	-20	P15056
61	BRCA1	BRCA1	BRCA1	672	Millipore	OP92	898	AB_2750876	Mouse	Caution	1:1000	4	P38398
62	BRD4	BRD4	BRD4	23476	CST	13440	1567	AB_2687578	Rabbit	Valid	1:1500	-20	O60885
63	c-Abl	c-Abl	ABL1	25	CST	2862	1565	AB_2257757	Rabbit	Valid	1:40	-20	P00519
64	c-Abl (phospho Tyr412)	c-Abl_pY412	ABL1	25	CST	2865	1146	AB_331381	Rabbit	Caution	1:60	-20	P00519
65	Cadherin-6	Cadherin-6	CDH6	1004	CST	48111	2630	AB_2799334	Rabbit	Caution	1:250	-20	P55285
66	Calnexin	Calnexin	CANX	821	CST	2679	2407	AB_2228381	Rabbit	Valid	1:200	-20	P27824
67	Caspase 3 (cleaved Asp175)	Caspase-3-cleaved	CASP3	836	CST	9661	1003	AB_2341188	Rabbit	Caution	1:37	-20	P42574
68	Caspase 7 (cleaved Asp198)	Caspase-7-cleaved	CASP7	840	CST	9491	109	AB_2068144	Rabbit	Caution	1:100	-20	P55210
69	Caspase 8 (cleaved Asp391)	Caspase-8-cleaved	CASP8	841	CST	9496	1165	AB_561381	Rabbit	Caution	1:30	-20	Q14790
70	Caveolin-1	Caveolin-1	CAV1	857	CST	3238	114	AB_2072166	Rabbit	Valid	1:3000	-20	Q03135
71	CD134/OX40	CD134	TNFRSF4	7293	CST	61637	2529	AB_2799611	Rabbit	Valid	1:30	-20	P43489
72	CD171	CD171	L1CAM	3897	Biolegend	826701	1737	AB_2564904	Mouse	Valid	1:150	4	P32004
73	CD2	CD2	CD2	914	Santa Cruz	sc-18907	1923	AB_626961	Mouse	Caution	1:20	4	P06729
74	CD20	CD20	MS4A1	931	Invitrogen	PA5-16701	2383	AB_10980806	Rabbit	Caution	1:50	4	P11836
75	CD26/DPP4	CD26	DPP4	1803	Abcam	ab28340	1308	AB_726291	Rabbit	Valid	1:1000	-20	P27487
76	CD4	CD4	CD4	920	Abcam	ab133616	2104	AB_2750883	Rabbit	Valid	1:20	-20	P01730
77	CD44	CD44	CD44	960	CST	37259	2384	AB_2750879	Rabbit	Caution	1:200	-20	P16070
78	CD45/T200/LCA	CD45	PTPRC	5788	DAKO	M070101-2	1352	AB_2750861	Mouse	Valid	1:37	4	P08575
79	CD68	CD68	CD68	968	R&D Systems	MAB2040	2055	AB_2244436	Mouse	Caution	1:150	-20	P34810
80	cdc2 (phospho Tyr15)	cdc2_pY15	CDK1	983	CST	4539	1783	AB_560953	Rabbit	Caution	1:20	-20	P06493
81	cdc25C	cdc25C	CDC25C	995	CST	4688	1873	AB_560956	Rabbit	Valid	1:100	-20	P30307
82	Cdc6	Cdc6	CDC6	990	CST	3387	2396	AB_2078525	Rabbit	Valid	1:75	-20	Q99741
83	CDK1-3 (phospho Thr14)	CDK1_pT14	CDK1	983	Abcam	ab32384	1658	AB_726756	Rabbit	Caution	1:1000	-20	P06493
			CDK2	1017									P24941
			CDK3	1018									Q00526
84	CDK9	CDK9	CDK9	1025	CST	2316	2406	AB_2291505	Rabbit	Valid	1:1500	-20	P50750
85	CDT1	CDT1	CDT1	81620	CST	8064	2397	AB_10896851	Rabbit	Valid	1:30	-20	Q9H211
86	CENP-A	CENP-A	CENPA	1058	CST	2186	2405	AB_10828491	Rabbit	Valid	1:500	-20	P49450
87	CHD1L	CHD1L	CHD1L	9557	CST	13460	2410	AB_2798225	Rabbit	Valid	1:250	-20	Q86WJ1
88	Chk1	Chk1	CHEK1	1111	CST	2360	1203	AB_2080320	Mouse	Caution	1:50	-20	O14757
89	Chk1 (phospho S296)	Chk1_pS296	CHEK1	1111	Abcam	ab79758	1348	AB_2244917	Rabbit	Valid	1:75	-20	O14757
90	Chk1 (phospho Ser345)	Chk1_pS345	CHEK1	1111	CST	2348	903	AB_331212	Rabbit	Caution	1:50	-20	O14757
91	Chk2	Chk2	CHEK2	11200	CST	3440	146	AB_2229490	Mouse	Valid	1:30	-20	O96017
92	Chk2 (phospho Thr68)	Chk2_pT68	CHEK2	11200	CST	2197	147	AB_2080501	Rabbit	Caution	1:150	-20	O96017
93	c-IAP2	c-IAP2	BIRC3	330	CST	3130	1615	AB_10693298	Rabbit	Caution	1:100	-20	Q13489
94	c-Jun (phospho Ser73)	c-Jun_pS73	JUN	3725	CST	3270	2417	AB_2129575	Rabbit	Valid	1:300	-20	P05412
95	c-Kit	c-Kit	KIT	3815	Abcam	ab216450	2453	AB_2868615	Rabbit	Valid	1:250	-20	P10721
96	Claudin-7	Claudin-7	CLDN7	1366	Millipore Sigma	ABT47	2356	AB_10851743	Rabbit	Valid	1:1500	4	O95471
97	c-Met (phospho Tyr1234 Tyr1235)	c-Met_pY1234_Y1235	MET	4233	CST	3129	727	AB_561173	Rabbit	Valid	1:100	-20	P08581
98	c-Myc [discontinued]	c-Myc	MYC	4609	Santa Cruz	sc-764	1143	AB_631276	Rabbit	Caution	1:250	4	P01106
99	COG3	COG3	COG3	83548	ProteinTech	11130-1-AP	1656	AB_2081402	Rabbit	Valid	1:750	-20	Q96JB2
100	Collagen VI/COL6A1 [discontinued]	Collagen-VI	COL6A1	1291	Santa Cruz	sc-20649	171	AB_2083098	Rabbit	Valid	1:6000	4	P12109
101	Connexin 43	Connexin-43	GJA1	2697	CST	3512	1568	AB_2294590	Rabbit	Caution	1:150	-20	P17302
102	Coup-TFII	Coup-TFII	NR2F2	7026	CST	6434	1571	AB_11220428	Rabbit	Caution	1:37	-20	P24468
103	COX IV	Cox-IV	COX4I1	1327	CST	4850	1116	AB_2085424	Rabbit	Valid	1:7500	-20	P13073
104	Cox2	Cox2	PTGS2	5743	CST	12282	2562	AB_2571729	Rabbit	Valid	1:125	-20	D9MWI3
105	CRABP2	CRABP2	CRABP2	1382	Novus Biologicals	NB100-2590	2479	AB_608440	Rabbit	Valid	1:1000	4	P29373
106	C-Raf ( phospho Ser338)	C-Raf_pS338	RAF1	5894	CST	9427	179	AB_2067317	Rabbit	Valid	1:75	-20	P04049
107	Creb	Creb	CREB1	1385	CST	9197	181	AB_331277	Rabbit	Caution	1:30	-20	P16220
108	Creb (phospho Ser133)	Creb_pS133	CREB1	1385	CST	9198	2334	AB_2561044	Rabbit	Caution	1:200	-20	P16220
109	Csk	Csk	CSK	1445	CST	4980	2155	AB_2276592	Rabbit	Caution	1:75	-20	P41240
110	CtIP	CtIP	RBBP8	5932	CST	9201	2160	AB_10828593	Rabbit	Valid	1:75	-20	Q99708
111	Cyclin B1	Cyclin-B1	CCNB1	891	Epitomics/Abcam	1495-1/ab32053	192	AB_731779	Rabbit	Valid	1:1500	-20	P14635
112	Cyclin D1	Cyclin-D1	CCND1	595	Millipore Sigma	SAB4502603	2299	AB_2750911	Rabbit	Caution	1:75	-20	P24385
113	Cyclin D3	Cyclin-D3	CCND3	896	CST	2936	198	AB_2070801	Mouse	Valid	1:1000	-20	P30281
114	Cyclin E1	Cyclin-E1	CCNE1	898	CST	20808	2420	AB_2783554	Rabbit	Valid	1:75	-20	P24864
115	Cyclophilin F	Cyclophilin-F	PPIF	10105	Abcam	ab110324	1257	AB_10864110	Mouse	Valid	1:75000	4	P30405
116	DAP Kinase 1 (phospho S308)	DAPK1_pS308	DAPK1	1612	GeneTex	GTX10524	1770	AB_381070	Mouse	Caution	1:200	-20	P53355
117	DDB-1	DDB-1	DDB1	1642	CST	6998	1980	AB_10829458	Rabbit	Valid	1:5000	-20	Q16531
118	DDR1	DDR1	DDR1	780	CST	5583	2402	AB_10694842	Rabbit	Valid	1:250	-20	Q08345
119	DDR1 (phospho Tyr513)	DDR1_pY513	DDR1	780	Millipore Sigma	SAB4504671	2403	AB_2868545	Rabbit	Caution	1:10000	-20	Q08345
120	DNA Ligase IV	DNA-Ligase-IV	LIG4	3981	CST	14649	2030	AB_2750871	Rabbit	Caution	1:250	-20	P49917
121	DNA Polymerase gamma	DNA-POLG	POLG	5428	CST	13609	2371	AB_2750886	Rabbit	Valid	1:250	-20	P54098
122	DNMT1	DNMT1	DNMT1	1786	CST	5032	2344	AB_10548197	Rabbit	Valid	1:300	-20	P26358
123	DRP1	DRP1	DNM1L	10059	CST	5391	2373	AB_11178938	Rabbit	Valid	1:1500	-20	O00429
124	DUSP4/MKP2	DUSP4	DUSP4	1846	CST	5149	1406	AB_2750867	Rabbit	Valid	1:150	-20	Q13115
125	DUSP6	DUSP6	DUSP6	1848	Abcam	ab76310	1936	AB_1523517	Rabbit	Caution	1:500	-20	Q16828
126	Dvl3	Dvl3	DVL3	1857	CST	3218	940	AB_10694060	Rabbit	Valid	1:30	-20	Q92997
127	DYRK1B	DYRK1B	DYRK1B	9149	CST	5672	2551	AB_10705603	Rabbit	Caution	1:500	-20	Q9Y463
128	E2F-1	E2F1	E2F1	1869	CST	3742	2466	AB_2096936	Rabbit	Valid	1:50	-20	Q01094
129	E-Cadherin	E-Cadherin	CDH1	999	GeneTex	GTX33614	2514	AB_2801514	Rabbit	Valid	1:500	-20	P12830
130	eEF2	eEF2	EEF2	1938	CST	2332	1060	AB_10693546	Rabbit	Caution	1:50	-20	P13639
131	eEF2K	eEF2K	EEF2K	29904	CST	3692	1061	AB_10694413	Rabbit	Valid	1:50	-20	O00418
132	EGF Receptor	EGFR	EGFR	1956	CST	2232	1120	AB_331707	Rabbit	Valid	1:100	-20	P00533
133	EGF Receptor (phospho Tyr1173)	EGFR_pY1173	EGFR	1956	Epitomics/Abcam	1124-1/ab32578	221	AB_873776	Rabbit	Valid	1:300	-20	P00533
134	eIF4E	eIF4E	EIF4E	1977	CST	9742	722	AB_823488	Rabbit	Valid	1:75	-20	P06730
135	eIF4E (phospho Ser209)	eIF4E_pS209	EIF4E	1977	Abcam	ab76256	1871	AB_1523534	Rabbit	Valid	1:150	-20	P06730
136	eIF4G	eIF4G	EIF4G1	1981	CST	2498	1124	AB_2096025	Rabbit	Caution	1:1000	-20	Q04637
137	Elk-1 (phospho Ser383)	Elk1_pS383	ELK1	2002	CST	9181	228	AB_2099016	Rabbit	Caution	1:37	-20	P19419
138	Enolase-1	Enolase-1	ENO1	2023	CST	3810	2337	AB_2246524	Rabbit	Valid	1:50	-20	I0J1J1
139	Enolase-2	Enolase-2	ENO2	2026	CST	8171	2338	AB_11178392	Rabbit	Valid	1:100	-20	P09104
140	ENY2	ENY2	ENY2	56943	GeneTex	GTX629542	1219	AB_2721898	Mouse	Caution	1:250	-20	Q9NPA8
141	EphA2	EphA2	EPHA2	1969	CST	6997	2421	AB_10827743	Rabbit	Valid	1:250	-20	P29317
142	EphA2 (phoshpo Tyr588)	EphA2_pY588	EPHA2	1969	CST	12677	2423	AB_2797989	Rabbit	Caution	1:250	-20	P29317
143	EphA2 (phospho Ser897)	EphA2_pS897	EPHA2	1969	CST	6347	2422	AB_11220420	Rabbit	Caution	1:250	-20	P29317
144	Epithelial Membrane Antigen	EMA	MUC1	4582	DAKO	M061329-2	1350	AB_2750907	Mouse	Caution	1:750	4	P15941
145	ErbB2/Her2 (phospho Tyr1248)	HER2_pY1248	ERBB2	2064	R&D Systems	AF1768	1075	AB_416537	Rabbit	Valid	1:300	-20	P04626
146	ErbB3/HER3 (phospho Y1289)	HER3_pY1289	ERBB3	2065	CST	4791	728	AB_2099709	Rabbit	Caution	1:50	-20	P21860
147	ErbB3/HER3 [discontinued]	HER3	ERBB3	2065	Santa Cruz	sc-285	911	AB_2099723	Rabbit	Valid	1:300	4	P21860
148	ERCC1	ERCC1	ERCC1	2067	CST	5437	2549	AB_10831838	Rabbit	Caution	1:250	-20	P07992
149	ERCC5/XPG	ERCC5	ERCC5	2073	Proteintech Group	11331-1-AP	1355	AB_2098155	Rabbit	Caution	1:250	-20	P28715
150	Erk5-MAPK7	Erk5	MAPK7	5598	CST	3552	2193	AB_2297353	Rabbit	Valid	1:150	-20	Q13164
151	Erk5-MAPK7 (phospho Thr218 Tyr220)	BMK1-Erk5_pT218_Y220	MAPK7	5598	Millipore	07-507	2194	AB_310675	Rabbit	Valid	1:500	4	Q13164
152	ERRalpha	ERRalpha	ESRRA	2101	CST	13826	2369	AB_2750873	Rabbit	Valid	1:250	-20	P11474
153	Estrogen Receptor alpha	ER-a	ESR1	2099	CST	13258	1942	AB_2632959	Rabbit	Valid	1:37	-20	P03372
154	Estrogen Receptor alpha (phospho Ser118)	ER-a_pS118	ESR1	2099	Epitomics/Abcam	1091-1/ab32396	241	AB_732252	Rabbit	Valid	1:500	-20	P03372
155	Ets-1	Ets-1	ETS1	2113	Bethyl	A303-501A	1200	AB_10951836	Rabbit	Valid	1:100	4	P14921
156	EVI1	EVI1	MECOM	2122	CST	2593	2492	AB_2184098	Rabbit	Valid	1:300	-20	Q03112
157	Ezh2	Ezh2	EZH2	2146	CST	5246	2641	AB_10694683	Rabbit	Valid	1:250	-20	Q15910
158	FABP5	FABP5	FABP5	2171	Proteintech	12348-1-AP	2480	AB_2100341	Rabbit	Caution	1:750	-20	Q01469
159	FAK	FAK	PTK2	5747	CST	3285	1228	AB_2269034	Rabbit	Caution	1:125	-20	Q05397
160	FAK (phospho Tyr397)	FAK_pY397	PTK2	5747	CST	8556	2457	AB_10891442	Rabbit	Valid	1:200	-20	Q05397
161	Fatty Acid Synthase	FASN	FASN	2194	CST	3180	1156	AB_2100796	Rabbit	Valid	1:1000	-20	A0A0U1RQF0
162	FGF-basic	FGF-basic	FGF2	2247	Peprotech/VWR	500-P18/10775-082	2085	AB_2750874	Rabbit	Caution	1:1000	-20	P09038
163	FGFR1	FGFR1	FGFR1	2260	CST	9740	2618	AB_11178519	Rabbit	Valid	1:250	-20	P11362
164	FGFR2	FGFR2	FGFR2	2263	CST	11835	2619	AB_2797742	Rabbit	Valid	1:100	-20	P21802
165	Fibronectin	Fibronectin	FN1	2335	Epitomics/Abcam	1574-1/ab45688	262	AB_732380	Rabbit	Valid	1:20000	-20	P02751
166	Folate Binding Protein	Folate-Binding-Protein	FOLR1	2348	Abcam	ab221543	2631	AB_2920895	Rabbit	Valid	1:250	-20	P15328
167	Folliculin	Folliculin	FLCN	201163	CST	3697	2599	AB_2231646	Rabbit	Valid	1:500	-20	Q8NFG4
168	FoxM1	FOXM1	FOXM1	2305	CST	20459	2458	AB_2798842	Rabbit	Valid	1:100	-20	Q08050
169	FoxO3a	FoxO3	FOXO3	2309	CST	12829	2289	AB_2636990	Rabbit	Valid	1:300	-20	O43524
170	FoxO3a (phospho Ser318 Ser321)	FoxO3a_pS318_S321	FOXO3	2309	Biorbyt	orb6051	2515	AB_10930919	Rabbit	Caution	1:1000	-20	O43524
171	FRS2-alpha (phospho Tyr196)	FRS2-alpha_pY196	FRS2	10818	Invitrogen	PA5-64616	2475	AB_2664213	Rabbit	Valid	1:500	-20	Q8WU20
172	FTO	FTO	FTO	79068	Abcam	ab124892	2575	AB_10972698	Rabbit	Valid	1:750	-20	Q9C0B1
173	G6PD	G6PD	G6PD	2539	CST	12263	2446	AB_2797861	Rabbit	Valid	1:75	-20	P11413
174	Gab2	Gab2	GAB2	9846	CST	80884	2640	AB_2923519	Rabbit	Valid	1:100	-20	Q9UQC2
175	GAPDH	GAPDH	GAPDH	2597	Invitrogen	AM4300	274	AB_2536381	Mouse	Caution	1:65000	-20	P04406
176	GATA3	GATA3	GATA3	2625	BD Biosciences	558686	764	AB_2108590	Mouse	Valid	1:150	4	P23771
177	GATA6	GATA6	GATA6	2627	CST	5851	2309	AB_10705521	Rabbit	Valid	1:30	-20	Q92908
178	GCLC	GCLC	GCLC	2729	Proteintech Group	12601-1-AP	1634	AB_2278734	Rabbit	Caution	1:500	-20	P48506
179	GCLM	GCLM	GCLM	2730	Invitrogen	MA5-32783	2496	AB_2800360	Rabbit	Valid	1:75	-20	P48507
180	GCN5L2	GCN5L2	KAT2A	2648	CST	3305	1263	AB_2128281	Rabbit	Valid	1:30	-20	Q92830
181	GGPS1	GGPS1	GGPS1	9453	Santa Cruz	sc-271680	2498	AB_10708412	Mouse	Valid	1:500	4	O95749
182	GLI1	Gli1	GLI1	2735	CST	3538	1579	AB_1903989	Rabbit	Caution	1:3000	-20	P08151
183	Gli3	Gli3	GLI3	2737	Abcam	ab181130	2588	AB_2732891	Rabbit	Valid	1:100	-20	P10071
184	Glucocorticoid Receptor	Glucocorticoid-Receptor	NR3C1	2908	CST	3660	2530	AB_11179215	Rabbit	Valid	1:1000	-20	P04150
185	Glutamate Dehydrogenase1/2	Glutamate-D1-2	GLUD1	2746	Novus	NBP2-16679	2320	AB_2750902	Rabbit	Valid	1:500	-20	P00367
186	Glutaminase	Glutaminase	GLS	2744	Abcam	ab156876	1491	AB_2721038	Rabbit	Caution	1:50	-20	O94925
187	Glycogen Synthase	Gys	GYS1	2997	CST	3886	1035	AB_2116392	Rabbit	Valid	1:2000	-20	P13807
188	Glycogen Synthase (phospho Ser641)	Gys_pS641	GYS1	2997	CST	3891	1036	AB_2116390	Rabbit	Valid	1:300	-20	P13807
189	Granzyme B	Granzyme-B	GZMB	3002	CST	4275	1807	AB_2114432	Rabbit	Valid	1:100	-20	P10144
190	GRB2	GRB2	GRB2	2885	CST	3972	1094	AB_10693935	Rabbit	Valid	1:750	-20	P62993
191	GRB7	GRB7	GRB7	2886	Abcam	ab183737	2169	AB_2750904	Rabbit	Valid	1:750	-20	Q14451
192	Grp75	Grp75	HSPA9	3313	CST	3593	2339	AB_2120328	Rabbit	Caution	1:150	-20	P38646
193	GSK-3alpha/beta	GSK-3a-b	GSK3A	2931	Santa Cruz	sc-7291	284	AB_2279451	Mouse	Valid	1:250	4	P49840
193	GSK-3alpha/beta	GSK-3a-b	GSK3B	2932	Santa Cruz	sc-7291	284	AB_2279451	Mouse	Valid	1:250	4	P49841
194	GSK-3alpha/beta (phospho Ser21 Ser9)	GSK-3a-b_pS21_S9	GSK3A	2931	CST	9331	285	AB_329830	Rabbit	Valid	1:200	-20	P49840
194	GSK-3alpha/beta (phospho Ser21 Ser9)	GSK-3a-b_pS21_S9	GSK3B	2932	CST	9331	285	AB_329830	Rabbit	Valid	1:200	-20	P49841
195	GSK-3beta	GSK-3B	GSK3B	2932	CST	9315	1163	AB_490890	Rabbit	Caution	1:750	-20	P49841
196	Hamartin/TSC1	TSC1	TSC1	7248	CST	6935	2553	AB_10860420	Rabbit	Valid	1:100	-20	Q92574
197	HER-2/c-erbB-2/neu Ab-8	HER2	ERBB2	2064	Lab Vision/ThermoFisher	MS-325-P1	1038	AB_61444	Mouse	Valid	1:300	4	P04626
198	Heregulin	Heregulin	NRG1	3084	CST	2573	890	AB_1031011	Rabbit	Valid	1:50	-20	Q02297
199	HES1	HES1	HES1	3280	CST	11988	1582	AB_2728766	Rabbit	Valid	1:500	-20	Q14469
200	Hexokinase I	Hexokinase-I	HK1	3098	CST	2024	2340	AB_2116996	Rabbit	Caution	1:500	-20	P19367
201	Hexokinase II	Hexokinase-II	HK2	3099	Abcam	ab228819	2435	AB_2868547	Rabbit	Valid	1:4000	-20	P52789
202	HIF-1A	Hif-1-alpha	HIF1A	3091	CST	36169	2392	AB_2799095	Rabbit	Caution	1:150	-20	Q16665
203	Histone H2AX (phospho Ser139)	H2AX_pS139	H2AX	3014	CST	9718	1342	AB_2118009	Rabbit	Caution	1:50	-20	P16104
204	Histone H2AX (phospho Ser140)	H2AX_pS140	H2AX	3014	Abcam	ab22551	2129	AB_447150	Mouse	Caution	1:500	-20	P16104
205	Histone H2B (ubiquityl Lys120)	U-Histone-H2B	H2BC3	3018	CST	5546	1662	AB_10693452	Rabbit	Caution	1:500	-20	P33778
206	Histone H3	Histone-H3	H3C1	8350	Abcam	ab1791	1250	AB_302613	Rabbit	Valid	1:5000	-20	P68431
			H3C4	8351
			H3C3	8352
			H3C6	8353
			H3C11	8354
			H3C8	8355
			H3C12	8356
			H3C10	8357
			H3C2	8358
			H3C7	8968
207	Histone H3 (dimethyl)	DM-Histone-H3	H3-4	8290	Millipore	07-030	1380	AB_310342	Rabbit	Valid	1:1000	-20	Q16695
208	Histone H3 (phospho Ser10)	Histone-H3_pS10	H3C1	8350	CST	3377	2341	AB_1549592	Rabbit	Valid	1:200	-20	P68431
			H3C2	8358
			H3C3	8352
			H3C4	8351
			H3C6	8353
			H3C7	8968
			H3C8	8355
			H3C10	8357
			H3C11	8354
			H3C12	8356
209	HMHA1	HMHA1	ARHGAP45	23526	ProteinTech	14832-1-AP	1902	AB_10642145	Rabbit	Valid	1:3000	-20	Q92619
210	hnRNP K	HNRNPK	HNRNPK	3190	CST	9081	2469	AB_11178946	Rabbit	Valid	1:150	-20	P61978
211	HSP27	HSP27	HSPB1	3315	CST	2402	321	AB_331761	Mouse	Caution	1:75	-20	P04792
212	HSP27 (phospho Ser82)	HSP27_pS82	HSPB1	3315	CST	2401	323	AB_331644	Rabbit	Valid	1:37	-20	P04792
213	HSP60	HSP60	HSPD1	3329	CST	12165	1808	AB_2636980	Rabbit	Valid	1:250	-20	P10809
214	Hsp75/TRAP1	TRAP1	TRAP1	10131	BD Biosciences	612344	2370	AB_399710	Mouse	Valid	1:500	-20	Q12931
215	IDO	IDO	IDO1	3620	CST	86630	2158	AB_2636818	Rabbit	Caution	1:25	-20	P14902
216	IGF-1 Receptor beta	IGFRb	IGF1R	3480	CST	3027	336	AB_2122378	Rabbit	Caution	1:250	-20	P08069
217	IGF-1 Receptor beta (phospho Tyr1135 Tyr1136)	IGF1R_pY1135_Y1136	IGF1R	3480	CST	3024	1221	AB_331253	Rabbit	Valid	1:30	-20	P08069
217	IGF-1 Receptor beta (phospho Tyr1135 Tyr1136)	IGF1R_pY1135_Y1136	INSR	3643	CST	3024	1221	AB_331253	Rabbit	Valid	1:30	-20	P06213
218	IGFBP2	IGFBP2	IGFBP2	3485	CST	3922	335	AB_2123207	Rabbit	Valid	1:30	-20	P18065
219	IGFBP3	IGFBP3	IGFBP3	3486	BD Biosciences	611504	793	AB_398964	Mouse	Valid	1:30	-20	P17936
220	IL-6	IL-6	IL6	3569	CST	12153	1949	AB_2687897	Rabbit	Caution	1:50	-20	P05231
221	INPP4b	INPP4b	INPP4B	8821	CST	14543	2459	AB_2798505	Rabbit	Caution	1:300	-20	O15327
222	Insulin Receptor beta	IR-b	INSR	3643	CST	3025	1586	AB_2280448	Rabbit	Caution	1:30	-20	P06213
223	IRF-1	IRF-1	IRF1	3659	CST	8478	1387	AB_10949108	Rabbit	Valid	1:150	-20	P10914
224	IRF-3	IRF-3	IRF3	3661	CST	4302	2519	AB_1904036	Rabbit	Valid	1:250	-20	Q14653
225	IRS1	IRS1	IRS1	3667	Millipore	06-248	802	AB_2127890	Rabbit	Valid	1:100	-20	P35568
226	IRS2	IRS2	IRS2	8660	CST	4502	371	AB_2125774	Rabbit	Caution	1:50	-20	Q9Y4H2
227	JAB1	JAB1	COPS5	10987	Santa Cruz	sc-13157	1394	AB_627835	Mouse	Caution	1:20	4	Q92905
228	Jagged1	Jagged1	JAG1	182	Abcam	ab109536	1413	AB_10862281	Rabbit	Valid	1:50	-20	P78504
229	Jak2	Jak2	JAK2	3717	CST	3230	1166	AB_2128522	Rabbit	Valid	1:3000	-20	O60674
230	JNK (phospho Thr183 Tyr185)	JNK_pT183_Y185	MAPK8	5599	CST	4671	377	AB_331338	Rabbit	Caution	1:75	-20	P45983
231	KAP1	KAP1	TRIM28	10155	Abcam	ab10484	1757	AB_297223	Rabbit	Valid	1:2000	-20	Q13263
232	KEAP1	KEAP1	KEAP1	9817	CST	8047	2546	AB_10860776	Rabbit	Valid	1:500	-20	Q14145
233	LAD1	LAD1	LAD1	3898	Atlas/Sigma-Aldrich	HPA028732	2237	AB_10602916	Rabbit	Valid	1:250	-20	O00515
234	Lasu1/Ureb1	Lasu1	HUWE1	10075	Bethyl	IHC-00439	1643	AB_1907308	Rabbit	Valid	1:500	4	Q7Z6Z7
235	LC3A/B	LC3A-B	MAP1LC3A	84557	CST	4108	1618	AB_2137703	Rabbit	Caution	1:150	-20	Q9H492
235	LC3A/B	LC3A-B	MAP1LC3B	81631	CST	4108	1618	AB_2137703	Rabbit	Caution	1:150	-20	Q9GZQ8
236	Lck	Lck	LCK	3932	CST	2752	397	AB_2234649	Rabbit	Valid	1:75	-20	P06239
237	LCN2	LCN2	LCN2	3934	CST	44058	2545	AB_2799257	Rabbit	Valid	1:2000	-20	P80188
238	LDHA	LDHA	LDHA	3939	CST	3582	976	AB_2066887	Rabbit	Caution	1:100	-20	P00338
239	LRP6 (phospho Ser1490)	LRP6_pS1490	LRP6	4040	CST	2568	1367	AB_2139327	Rabbit	Valid	1:150	-20	O75581
240	Lyn	Lyn	LYN	4067	CST	2796	2424	AB_2138391	Rabbit	Valid	1:125	-20	P07948
241	MACC1	MACC1	MACC1	346389	CST	86290	2544	AB_2800075	Rabbit	Valid	1:500	-20	Q6ZN28
242	Mcl-1	Mcl-1	MCL1	4170	CST	5453	1222	AB_10694494	Rabbit	Valid	1:100	-20	Q07820
243	MDM2 (phospho Ser166)	MDM2_pS166	MDM2	4193	Abcam	ab131355	2600	AB_11157309	Rabbit	Valid	1:2000	-20	Q00987
244	MEK1	MEK1	MAP2K1	5604	Epitomics/Abcam	1235-1/ab32576	417	AB_776274	Rabbit	Valid	1:1500	-20	Q02750
245	MEK1/2 (phospho Ser217 Ser221)	MEK1_pS217_S221	MAP2K1	5604	CST	9154	1076	AB_2138017	Rabbit	Valid	1:50	-20	Q02750
245	MEK1/2 (phospho Ser217 Ser221)	MEK1_pS217_S221	MAP2K2	5605	CST	9154	1076	AB_2138017	Rabbit	Valid	1:50	-20	P36507
246	MERIT40	MERIT40	BABAM1	29086	CST	12711	1951	AB_2750885	Rabbit	Caution	1:3000	-20	Q9NWV8
247	MERIT40 (phospho Ser29)	MERIT40_pS29	BABAM1	29086	CST	12110	1952	AB_2750884	Rabbit	Valid	1:100	-20	Q9NWV8
248	Merlin/NF2	Merlin	NF2	4771	Novus	22710002	1046	AB_10004662	Rabbit	Caution	1:500	-20	P35240
249	METTL3	METTL3	METTL3	56339	Abcam	ab195352	2574	AB_2721254	Rabbit	Valid	1:1500	-20	Q86U44
250	Midkine	Midkine	MDK	4192	Invitrogen	MA5-32538	2539	AB_2809815	Rabbit	Valid	1:100	-20	P21741
251	MIF	MIF	MIF	2563	CST	88186	2563	AB_2800118	Rabbit	Valid	1:750	-20	P14174
252	MIG6	MIG6	ERRFI1	54206	CST	2440	2522	AB_823573	Rabbit	Valid	1:20	-20	Q9UJM3
253	MITF	MITF	MITF	4286	CST	12590	2365	AB_2616024	Rabbit	Valid	1:100	-20	O75030
254	Mitofusin-1	Mitofusin-1	MFN1	55669	CST	14739	1809	AB_2744531	Rabbit	Valid	1:500	-20	Q8IWA4
255	Mitofusin-2	Mitofusin-2	MFN2	9927	CST	11925	1810	AB_2750893	Rabbit	Valid	1:1000	-20	O95140
256	MLH1	MLH1	MLH1	4292	CST	3515	2324	AB_2145615	Mouse	Valid	1:500	-20	P40692
257	MLKL	MLKL	MLKL	197259	CST	14993	1780	AB_2721822	Rabbit	Valid	1:1000	-20	Q8NB16
258	MMP14	MMP14	MMP14	4323	Abcam	ab51074	2360	AB_881234	Rabbit	Valid	1:1000	-20	P50281
259	Mnk1	Mnk1	MKNK1	8569	CST	2195	1005	AB_2235175	Rabbit	Valid	1:100	-20	Q9BUB5
260	Monocarboxylic Acid Transporter 4	MCT4	SLC16A3	9123	Millipore	AB3314P	1633	AB_2286063	Rabbit	Valid	1:500	-20	O15427
261	MRAP	MRAP	MRAP	56246	GeneTex	GTX53669	2645	AB_2933959	Rabbit	Valid	1:250	-20	Q8TCY5
262	MSH2	MSH2	MSH2	4436	CST	2017	2325	AB_2235387	Rabbit	Caution	1:750	-20	P43246
263	MSH6	MSH6	MSH6	2956	sdix/Novus	22030002	1063	AB_2266534	Rabbit	Caution	1:3000	-20	P52701
264	MSI2	MSI2	MSI2	124540	Abcam	ab76148	1675	AB_1523981	Rabbit	Caution	1:500	-20	Q96DH6
265	MTCO1	MTCO1	MT-CO1	4512	Abcam	ab14705	1344	AB_2084810	Mouse	Valid	1:500	4	P00395
266	mTOR	mTOR	MTOR	2475	CST	2983	444	AB_2105622	Rabbit	Valid	1:3000	-20	P42345
267	mTOR (phospho Ser2448)	mTOR_pS2448	MTOR	2475	CST	2971	446	AB_330970	Rabbit	Caution	1:50	-20	P42345
268	Myosin Heavy Chain 11	MYH11	MYH11	4629	GeneTex	GTX131414	2358	AB_2750872	Rabbit	Caution	1:500	-20	P35749
269	Myosin IIa	Myosin-IIa	MYH9	4627	CST	3403	1647	AB_2147297	Rabbit	Caution	1:300	-20	P35579
270	Myosin IIa (phospho Ser1943)	Myosin-IIa_pS1943	MYH9	4627	CST	5026	1160	AB_10576567	Rabbit	Valid	1:750	-20	P35579
271	Myt1	Myt1	PKMYT1	9088	CST	4282	1803	AB_2163831	Rabbit	Caution	1:50	-20	Q99640
272	N-Cadherin	N-Cadherin	CDH2	1000	CST	13116	2391	AB_2687616	Rabbit	Valid	1:20	-20	P19022
273	NDRG1 (phospho Thr346)	NDRG1_pT346	NDRG1	10397	CST	3217	1126	AB_2150174	Rabbit	Valid	1:100	-20	Q92597
274	NF-kappaB p65 (phospho Ser536)	NF-kB-p65_pS536	RELA	5970	CST	3033	457	AB_331284	Rabbit	Caution	1:30	-20	Q04206
275	Notch1	Notch1	NOTCH1	4851	CST	3608	2461	AB_2153354	Rabbit	Valid	1:200	-20	P46531
276	Notch1 (cleaved Val1744)	Notch1-cleaved	NOTCH1	4851	CST	4147	1969	AB_2153348	Rabbit	Valid	1:37	-20	P46531
277	Notch3 [discontinued]	Notch3	NOTCH3	4854	Santa Cruz	sc-5593	767	AB_2151246	Rabbit	Caution	1:500	4	Q9UM47
278	NQO1	NQO1	NQO1	1728	CST	3187	1420	AB_2154354	Mouse	Valid	1:1000	-20	P15559
279	N-Ras [discontinued]	N-Ras	NRAS	4893	Santa Cruz	sc-31	1136	AB_628041	Mouse	Valid	1:30	4	P01111
280	NRF2	NRF2	NFE2L2	4780	CST	12721	1419	AB_2715528	Rabbit	Caution	1:50	-20	Q16236
281	Oct-4	Oct-4	POU5F1	5460	CST	2750	1669	AB_823583	Rabbit	Caution	1:25	-20	Q01860
282	p21	p21	CDKN1A	1026	Invitrogen	MA5-14949	2316	AB_10984461	Rabbit	Caution	1:500	-20	P38936
283	p27 Kip 1 (phospho Thr198)	p27_pT198	CDKN1B	1027	Abcam	ab64949	878	AB_1142099	Rabbit	Valid	1:50	-20	P46527
284	p27 Kip1	p27-Kip1	CDKN1B	1027	Abcam	ab206927	2523	AB_2811037	Rabbit	Caution	1:1500	-20	P46527
285	p38 alpha MAPK	p38-a	MAPK14	1432	CST	9228	1175	AB_490886	Mouse	Valid	1:300	-20	Q16539
286	p38 MAPK	p38-MAPK	MAPK11	5600	CST	9212	478	AB_330713	Rabbit	Valid	1:1500	-20	Q15759
			MAPK12	6300									P53778
			MAPK14	1432									Q16539
287	p38 MAPK (phospho Thr180 Tyr182)	p38-MAPK_pT180_Y182	MAPK11	5600	CST	9215	850	AB_331762	Rabbit	Valid	1:20	-20	Q15759
			MAPK12	6300									P53778
			MAPK13	5603									O15264
			MAPK14	1432									Q16539
288	p44/42 MAPK Erk1/2	p44-42-MAPK	MAPK1	5594	CST	4695	1119	AB_390779	Rabbit	Valid	1:1500	-20	P28482
288	p44/42 MAPK Erk1/2	p44-42-MAPK	MAPK3	5595	CST	4695	1119	AB_390779	Rabbit	Valid	1:1500	-20	P27361
289	p44/42 MAPK Erk1/2 (phospho Thr202 Tyr204)	MAPK_pT202_Y204	MAPK1	5594	CST	4370	2460	AB_2315112	Rabbit	Caution	1:500	-20	P28482
289	p44/42 MAPK Erk1/2 (phospho Thr202 Tyr204)	MAPK_pT202_Y204	MAPK3	5595	CST	4370	2460	AB_2315112	Rabbit	Caution	1:500	-20	P27361
290	p53	p53	TP53	7157	CST	9282	481	AB_331476	Rabbit	Caution	1:2000	-20	P04637
291	p70 S6 Kinase	p70-S6K1	RPS6KB1	6198	Epitomics/Abcam	1494-1/ab32529	493	AB_777800	Rabbit	Valid	1:300	-20	P23443
292	p70 S6 Kinase (phospho Thr389)	p70-S6K_pT389	RPS6KB1	6198	CST	9205	494	AB_330944	Rabbit	Valid	1:50	-20	P23443
293	p73	p73	TP73	7161	Abcam	ab189896	2650	AB_2941918	Rabbit	Valid	1:500	-20	O15350
294	PAI-1	PAI-1	SERPINE1	5054	BD Biosciences	612024	499	AB_399419	Mouse	Valid	1:150	-20	P05121
295	PAICS	PAICS	PAICS	10606	Sigma-Aldrich	HPA035895	1322	AB_10669660	Rabbit	Caution	1:250	-20	P22234
296	PAK1	PAK1	PAK1	5058	CST	2602	1811	AB_330222	Rabbit	Valid	1:500	-20	Q13153
297	PAK1-2 (phospho Thr423 Thr402)	PAK_pT423_T402	PAK1	5058	CST	2601	2377	AB_330220	Rabbit	Caution	1:100	-20	Q13153
			PAK2	5062									Q13177
			PAK3	5063									O75914
298	PAK4	PAK4	PAK4	10298	Invitrogen	PA5-69540	2517	AB_2691394	Rabbit	Valid	1:200	-20	O96013
299	PAK4-6 (phospho Ser474 Ser602 Ser560)	PAK_pS474_S602_S560	PAK4	10298	CST	3241	2376	AB_2158623	Rabbit	Valid	1:500	-20	O96013
			PAK5	57144									Q9P286
			PAK6	56924									Q9NQU5
300	PAR [discontinued]	PAR	NA	NA	Trevigen	4336-BPC-100	1370	AB_2721257	Rabbit	Caution	1:30000	-20	NA
301	PARG	PARG	PARG	8505	CST	66564	2209	AB_2750890	Rabbit	Caution	1:75	-20	Q86W56
302	PARK7/DJ1	DJ1	PARK7	11315	Abcam	ab76008	891	AB_1310549	Rabbit	Valid	1:1000	-20	Q99497
303	PARP	PARP	PARP1	142	CST	9532	2185	AB_659884	Rabbit	Valid	1:1000	-20	P09874
304	PAX6	PAX6	PAX6	5080	CST	60433	2486	AB_2797599	Rabbit	Valid	1:20	-20	P26367
305	PAX8	PAX8	PAX8	7849	Novus Biologicals	NBP1-32440	2434	AB_2283498	Rabbit	Caution	1:500	-20	Q06710
306	Paxillin	Paxillin	PXN	5829	CST	2542	504	AB_10693603	Rabbit	Caution	1:200	-20	P49023
307	P-Cadherin	P-Cadherin	CDH3	1001	CST	2130	509	AB_2077669	Rabbit	Caution	1:25	-20	P22223
308	PCNA	PCNA	PCNA	5111	CST	2586	1383	AB_2160343	Mouse	Caution	1:100	-20	P12004
309	PD1	PD-1	PDCD1	5133	GeneTex	GTX128436	2443	AB_2814970	Rabbit	Valid	1:75	-20	Q15116
310	Pdcd4	Pdcd4	PDCD4	27250	Rockland	600-401-965	816	AB_828370	Rabbit	Caution	1:750	-20	Q53EL6
311	PDHK1	PDHK1	PDK1	5163	CST	3820	1622	AB_1904078	Rabbit	Caution	1:20	-20	Q15118
312	PDK1	PDK1	PDPK1	5170	CST	3062	515	AB_2236832	Rabbit	Valid	1:100	-20	O15530
313	PDK1 (phospho Ser241)	PDK1_pS241	PDPK1	5170	CST	3061	516	AB_2161919	Rabbit	Valid	1:50	-20	O15530
314	PD-L1	PD-L1	CD274	29126	CST	13684	1724	AB_2687655	Rabbit	Caution	1:50	-20	Q9NZQ7
315	PEA-15	PEA-15	PEA15	8682	CST	2780	1017	AB_2268149	Rabbit	Valid	1:100	-20	Q15121
316	PEA-15 (phopsho Ser116)	PEA-15_pS116	PEA15	8682	MyBioSource	MBS8241898	2664	AB_3076140	Rabbit	Valid	1:250	-20	Q15121
317	PERK	PERK	EIF2AK3	9451	CST	3192	2429	AB_2095847	Rabbit	Valid	1:250	-20	Q9NZJ5
318	PGM1	PGM1	PGM1	5236	Abcam	ab192876	2569	AB_2893201	Rabbit	Valid	1:250	-20	P36871
319	PHGDH	PHGDH	PHGDH	26227	CST	13428	1623	AB_2750870	Rabbit	Caution	1:500	-20	O43175
320	PHLPP	PHLPP	PHLPP1	23239	Proteintech	22789-1-AP	2399	AB_2750897	Rabbit	Valid	1:1500	-20	O60346
321	PI3 Kinase p110 alpha	PI3K-p110-a	PIK3CA	5290	CST	4255	808	AB_659888	Rabbit	Caution	1:150	-20	P42336
322	PI3 Kinase p85	PI3K-p85	PIK3R1	5295	Millipore	ABS1856	2277	AB_2750892	Rabbit	Caution	1:200	4	P27986
323	PIP4K2A	PIP4K2A	PIP4K2A	5305	CST	5527	2594	AB_2722636	Rabbit	Valid	1:500	-20	P48426
324	PIP4K2B	PIP4K2B	PIP4K2B	8396	CST	9694	2595	AB_2164572	Rabbit	Valid	1:250	-20	P78356
325	PKA RI-alpha	PKA-a	PRKAR1A	5573	CST	5675	1667	AB_10695452	Rabbit	Valid	1:250	-20	P10644
326	PKC (pan) (betaII) (phospho Ser660)	PKC-b-II_pS660	PRKCA	5578	CST	9371	1137	AB_2168219	Rabbit	Valid	1:200	-20	P17252
			PRKCB	5579									P05771
			PRKCD	5580									Q05655
			PRKCE	5581									Q02156
			PRKCH	5583									P24723
			PRKCQ	5588									Q04759
327	PKC alpha	PKCa	PRKCA	5578	CST	2056	1158	AB_2284227	Rabbit	Valid	1:100	-20	P17252
328	PKC alpha/beta II (phospho Thr638 Thr641)	PKC-a-b-II_pT638_T641	PRKCA	5578	CST	9375	1426	AB_2284224	Rabbit	Valid	1:250	-20	P17252
328	PKC alpha/beta II (phospho Thr638 Thr641)	PKC-a-b-II_pT638_T641	PRKCB	5579	CST	9375	1426	AB_2284224	Rabbit	Valid	1:250	-20	P05771
329	PKC delta (phospho Ser664)	PKC-delta_pS664	PRKCD	5580	Millipore	07-875	932	AB_568868	Rabbit	Valid	1:75	-20	Q05655
330	PKM2	PKM2	PKM	5315	CST	4053	1025	AB_1904096	Rabbit	Caution	1:150	-20	Q5NVN0
331	PLC gamma1	PLC-gamma1	PLCG1	5335	CST	5690	2489	AB_10691383	Rabbit	Valid	1:500	-20	P19174
332	PLC gamma1 (phospho Ser1248)	PLC-gamma1_pS1248	PLCG1	5335	CST	8713	2490	AB_10890863	Rabbit	Valid	1:500	-20	P19174
333	PLC gamma2 (phospho Tyr759)	PLC-gamma2_pY759	PLCG2	5336	GeneTex	GTX133463	2441	AB_2868555	Rabbit	Valid	1:2500	-20	P16885
334	PLK1	PLK1	PLK1	5347	CST	4513	754	AB_2167409	Rabbit	Caution	1:125	-20	P53350
335	PMS2	PMS2	PMS2	5395	Abcam	ab110638	2245	AB_10858491	Rabbit	Valid	1:500	-20	P54278
336	PRAS40	PRAS40	AKT1S1	84335	Invitrogen	AHO1031	738	AB_2536321	Mouse	Caution	1:75	-20	Q96B36
337	PRAS40 (phospho Thr246)	PRAS40_pT246	AKT1S1	84335	Invitrogen	44-1100G	739	AB_2533573	Rabbit	Valid	1:500	-20	Q96B36
338	PRC1 (phospho Thr481)	PRC1_pT481	PRC1	9055	Aviva Systems Biology	OAAF05458	2398	AB_2868544	Rabbit	Caution	1:150	-20	O43663
339	PREX1 [discontinued]	PREX1	PREX1	57580	Abcam	ab102739	1204	AB_10711640	Rabbit	Valid	1:100	-20	Q8TCU6
340	PRMT1	PRMT1	PRMT1	3276	CST	2449	1684	AB_2237696	Rabbit	Valid	1:500	-20	Q99873
341	PRMT5	PRMT5	PRMT5	10419	Abcam	ab109451	2609	AB_10863428	Rabbit	Valid	1:500	-20	O14744
342	Progesterone Receptor	PR	PGR	5241	abcam	ab206926	2296	AB_2750865	Rabbit	Valid	1:150	-20	P06401
343	PTEN	PTEN	PTEN	5728	CST	9552	566	AB_10694066	Rabbit	Valid	1:500	-20	P60484
344	PTPN12	PTPN12	PTPN12	5782	Abcam	ab76942	2154	AB_1524267	Rabbit	Valid	1:150	-20	Q05209
345	Puma/BBC3	Puma	BBC3	27113	CST	4976	1290	AB_2064551	Rabbit	Caution	1:50	-20	Q9BXH1
346	PYGB/Glycogen phosphorylase, brain	PYGB	PYGB	5834	Sigma-Aldrich	SAB2900066	1242	AB_10599750	Rabbit	Valid	1:500	-20	P11216
347	PYGM/Glycogen phosphorylase, muscle	PYGM	PYGM	5837	Novus/Abnova	H00005837-M10	1245	AB_2237986	Mouse	Caution	1:500	-20	P11217
348	Pyk2 (phospho Tyr402)	Pyk2_pY402	PTK2B	2185	CST	3291	1607	AB_2300530	Rabbit	Caution	1:75	-20	Q14289
349	Pyruvate Dehydrogenase	PDHA1	PDHA1	5160	CST	3205	1817	AB_2162926	Rabbit	Valid	1:50	-20	P08559
350	Rab11	Rab11	RAB11A	8766	CST	5589	2074	AB_10693925	Rabbit	Caution	1:500	-20	P62491
350	Rab11	Rab11	RAB11B	9230	CST	5589	2074	AB_10693925	Rabbit	Caution	1:500	-20	Q15907
351	Rab11FIP1	Rab11FIP1	RAB11FIP1	80223	CST	12849	2601	AB_2798044	Rabbit	Valid	1:500	-20	Q6WKZ4
352	Rab25	Rab25	RAB25	57111	CST	4314	1150	AB_10545758	Rabbit	Valid	1:30	-20	P57735
353	Rac1/Cdc42	Cdc42	CDC42	998	CST	4651	1332	AB_10612265	Rabbit	Caution	1:50	-20	P60953
353	Rac1/Cdc42	Cdc42	RAC1	5879	CST	4651	1332	AB_10612265	Rabbit	Caution	1:50	-20	P63000
354	Rad17 (phospho Ser645)	Rad17_pS645	RAD17	5884	CST	6981	2526	AB_11178527	Rabbit	Valid	1:300	-20	O75943
355	Rad23A	Rad23A	RAD23A	5886	CST	24555	1979	AB_2750888	Rabbit	Caution	1:3000	-20	P54725
356	Rad50	Rad50	RAD50	10111	CST	3427	1454	AB_2176936	Rabbit	Valid	1:150	-20	Q92878
357	Rad51	Rad51	RAD51	5888	Millipore	ABE257	2112	AB_10850319	Rabbit	Caution	1:2000	4	Q06609
358	Raf-1/C-Raf	C-Raf	RAF1	5894	Millipore	04-739	1201	AB_1977452	Rabbit	Caution	1:200	-20	P04049
359	Raptor	Raptor	RPTOR	57521	CST	2280	1128	AB_561245	Rabbit	Valid	1:300	-20	Q8N122
360	Rb	Rb	RB1	5925	CST	9309	552	AB_823629	Mouse	Red_Blob	1:150	-20	P06400
361	Rb (phospho Ser807 Ser811)	Rb_pS807_S811	RB1	5925	CST	9308	557	AB_331472	Rabbit	Valid	1:500	-20	P06400
362	RBM15	RBM15	RBM15	64783	Novus	21390002	1138	AB_2175759	Rabbit	Valid	1:5000	-20	Q96T37
363	Rheb	Rheb	RHEB	6009	R&D Systems	MAB3426	847	AB_2178785	Mouse	Caution	1:75	-20	Q15382
364	Rictor	Rictor	RICTOR	253260	CST	2114	1129	AB_2179963	Rabbit	Caution	1:100	-20	Q6R327
365	Rictor (phospho Thr1135)	Rictor_pT1135	RICTOR	253260	CST	3806	1130	AB_10557237	Rabbit	Valid	1:200	-20	Q6R327
366	RIP	RIP	RIPK1	8737	CST	4926	1624	AB_2224503	Rabbit	Caution	1:25	-20	Q13546
367	RPA32/RPA2	RPA32	RPA2	6118	CST	52448	2389	AB_2750889	Rabbit	Valid	1:500	-20	P15927
368	RPA32/RPA2 (phospho Ser4 Ser8)	RPA32_pS4_S8	RPA2	6118	Bethyl	A300-245A	1375	AB_210547	Rabbit	Caution	1:200	4	P15927
369	RRM2	RRM2	RRM2	6241	Life Technologies	PA5-27856	1981	AB_2545332	Rabbit	Caution	1:250	-20	P31350
370	RSK1	RSK1	RPS6KA1	6195	CST	8408	2425	AB_10828594	Rabbit	Valid	1:750	-20	Q15418
371	RSK1 (phospho Thr573)	p90RSK_pT573	RPS6KA1	6195	Invitrogen	PA5-37728	2448	AB_2554336	Rabbit	Caution	1:500	-20	Q15418
372	RSK1/2/3	RSK	RPS6KA1	6195	CST	9347	759	AB_330803	Rabbit	Caution	1:150	-20	Q15418
			RPS6KA2	6196									Q15349
			RPS6KA3	6197									P51812
373	S100A4	S100A4	S100A4	6275	CST	13018	2031	AB_2750896	Rabbit	Valid	1:200	-20	P26447
374	S6 Ribosomal Protein	S6	RPS6	6194	CST	2317	1874	AB_2238583	Mouse	Valid	1:750	-20	P62753
375	S6 Ribosomal Protein (phospho Ser235 Ser236)	S6_pS235_S236	RPS6	6194	CST	2211	600	AB_331679	Rabbit	Valid	1:10000	-20	P62753
376	S6 Ribosomal Protein (phospho Ser240 Ser244)	S6_pS240_S244	RPS6	6194	CST	2215	601	AB_331682	Rabbit	Valid	1:1000	-20	P62753
377	SCD	SCD	SCD	6319	Santa Cruz	sc-58420	1127	AB_785599	Mouse	Valid	1:20	4	O00767
378	SDHA	SDHA	SDHA	6389	CST	11998	1339	AB_2750900	Rabbit	Valid	1:250	-20	P31040
379	SFRP1	SFRP1	SFRP1	6422	CST	3534	2495	AB_2187065	Rabbit	Valid	1:100	-20	Q8N474
380	SGK1	SGK1	SGK1	6446	CST	12103	2411	AB_2687476	Rabbit	Valid	1:150	-20	O00141
381	SGK3	SGK3	SGK3	23678	CST	8156	2409	AB_10949507	Rabbit	Valid	1:200	-20	Q96BR1
382	Shc (phospho Tyr317)	Shc_pY317	SHC1	6464	Bioss	BS-3413R	2476	AB_10856715	Rabbit	Caution	1:750	-20	P29353
383	SHP-2/PTPN11	SHP2	PTPN11	5781	CST	3397	2241	AB_2174959	Rabbit	Valid	1:125	-20	Q06124
384	SHP-2/PTPN11 (phospho Tyr542)	SHP-2_pY542	PTPN11	5781	CST	3751	1180	AB_330825	Rabbit	Caution	1:75	-20	Q06124
385	SIRP alpha	SIRP-alpha	SIRPA	140885	Abcam	ab8120	2570	AB_306290	Rabbit	Valid	1:3000	-20	P78324
386	SLC1A5	SLC1A5	SLC1A5	6510	Sigma-Aldrich	HPA035240	1313	AB_10604092	Rabbit	Caution	1:150000	-20	Q15758
387	Slfn11 [discontinued]	Slfn11	SLFN11	91607	Santa Cruz	sc-374339	2136	AB_10989536	Mouse	Valid	1:150	4	Q7Z7L1
388	Smac/Diablo	Smac	DIABLO	56616	CST	2954	610	AB_2131196	Mouse	Red_Blob	1:150	-20	Q9NR28
389	Smad1	Smad1	SMAD1	4086	Epitomics/Abcam	1649-1/ab33902	922	AB_777975	Rabbit	Valid	1:250	-20	Q15797
390	Smad3	Smad3	SMAD3	4088	Epitomics/Abcam	1735-1/ab40854	796	AB_777979	Rabbit	Valid	1:150	-20	P84022
391	Smad4	Smad4	SMAD4	4089	CST	46535	2467	AB_2736998	Rabbit	Valid	1:500	-20	Q13485
392	Snail	Snail	SNAI1	6615	CST	3895	616	AB_2191759	Mouse	Red_Blob	1:50	-20	O95863
393	SOD1	SOD1	SOD1	6647	CST	4266	1818	AB_2193898	Mouse	Valid	1:500	-20	P00441
394	SOD2	SOD2	SOD2	6648	CST	13194	2291	AB_2750869	Rabbit	Valid	1:30	-20	P04179
395	Sox17	Sox17	SOX17	64321	Abcam	ab224637	2431	AB_2801385	Rabbit	Valid	1:500	-20	Q9H6I2
396	Sox2	Sox2	SOX2	6657	CST	2748	1670	AB_823640	Rabbit	Valid	1:37	-20	P48431
397	Sox7	Sox7	SOX7	83595	Novus Biologicals	NBP1-86116	2484	AB_11015890	Rabbit	Valid	1:75	-20	Q9BT81
398	Src	Src	SRC	6714	CST	2109	620	AB_2106059	Rabbit	Valid	1:1000	-20	P12931
399	Src (phospho Tyr527)	Src_pY527	SRC	6714	CST	2105	626	AB_331034	Rabbit	Valid	1:150	-20	P12931
400	Src Family (phospho Tyr416)	Src_pY416	SRC	6714	CST	6943	2463	AB_10013641	Rabbit	Valid	1:60	-20	P12931
			FYN	2534									P06241
			HCK	3055									P08631
			LCK	3932									P06239
			LYN	4067									P07948
			YES1	7525									P07947
401	Stat1 (phospho Tyr701)	Stat1_pY701	STAT1	6772	CST	9167	2264	AB_561284	Rabbit	Valid	1:50	-20	P42224
402	Stat3	Stat3	STAT3	6774	CST	4904	1197	AB_331269	Rabbit	Caution	1:5000	-20	P40763
403	Stat3 (phospho Tyr705)	Stat3_pY705	STAT3	6774	Fisher	44-380G	2321	AB_2533643	Rabbit	Caution	1:500	-20	P40763
404	Stat5a	Stat5a	STAT5A	6776	Epitomics/Abcam	1289-1/ab32043	638	AB_778107	Rabbit	Valid	1:300	-20	P42229
405	Stathmin-1	Stathmin-1	STMN1	3925	Epitomics/Abcam	1972-1/ab52630	718	AB_2197257	Rabbit	Valid	1:150	-20	P16949
406	STING	STING	STING1	340061	CST	13647	2099	AB_2732796	Rabbit	Valid	1:200	-20	Q86WV6
407	Syk	Syk	SYK	6850	Santa Cruz	sc-1240	1033	AB_628308	Mouse	Valid	1:300	4	P43405
408	Synaptophysin	Synaptophysin	SYP	6855	CST	36406	2547	AB_2799098	Rabbit	Caution	1:75	-20	P08247
409	Tau	Tau	MAPT	4137	Millipore	05-348	646	AB_309687	Mouse	Caution	1:100	-20	P10636
410	TAZ	TAZ	WWTR1	25937	CST	4883	1848	AB_1904158	Rabbit	Valid	1:300	-20	Q9GZV5
411	TCF8/ZEB1	ZEB1	ZEB1	6935	CST	3396	2412	AB_1904164	Rabbit	Valid	1:75	-20	P37275
412	TEAD	TEAD	TEAD1	7003	CST	13295	1934	AB_2687902	Rabbit	Caution	1:300	-20	P28347
			TEAD2	8463									Q15562
			TEAD3	7005									Q99594
			TEAD4	7004									Q15561
413	TFAM	TFAM	TFAM	7019	CST	7495	1333	AB_10841294	Rabbit	Valid	1:300	-20	Q00059
414	TIGAR	TIGAR	TIGAR	57103	Epitomics/Abcam	S1711/ab137573	1107	AB_2721901	Rabbit	Valid	1:300	-20	Q9NQ88
415	Transferrin Receptor	TFRC	TFRC	7037	Novus	22500002	1140	AB_10004660	Rabbit	Valid	1:10000	-20	P02786
416	Transglutaminase II	Transglutaminase	TGM2	7052	Lab Vision/Fisher	MS-224-P1	908	AB_62205	Mouse	Valid	1:150	4	P21980
417	TRIM24	TRIM24	TRIM24	8805	G-Biosciences	ITA0255	2541	AB_2818980	Rabbit	Caution	1:250	-20	O15164
418	TRIM25	TRIM25	TRIM25	7706	Abcam	ab167154	1756	AB_2721902	Rabbit	Caution	1:3000	-20	Q14258
419	TRIP13	TRIP13	TRIP13	9319	Invitrogen	PA5-52193	2400	AB_2648956	Rabbit	Valid	1:500	-20	Q15645
420	TROP2	TROP2	TACSTD2	4070	Abcam	ab214488	2627	AB_2811182	Rabbit	Valid	1:2000	-20	P09758
421	TTF1	TTF1	NKX2-1	7080	Epitomics/Abcam	2044-1/ab76013	1081	AB_1310784	Rabbit	Valid	1:125	-20	P43699
422	Tuberin	Tuberin	TSC2	7249	Epitomics/Abcam	1613-1/ab32554	670	AB_778686	Rabbit	Valid	1:1000	-20	P49815
423	Tuberin (phospho Thr1462)	Tuberin_pT1462	TSC2	7249	Abcam	ab109403	2511	AB_10861105	Rabbit	Valid	1:1500	-20	P49815
424	TUFM	TUFM	TUFM	7284	Invitrogen	PA5-27511	2390	AB_2544987	Rabbit	Valid	1:100	-20	P49411
425	TWEAK Receptor/FN14	FN14	TNFRSF12A	51330	CST	4403	1959	AB_10693941	Rabbit	Caution	1:50	-20	Q9NP84
426	Twist	Twist	TWIST1	7291	Santa Cruz	sc-81417	1353	AB_1130910	Mouse	Caution	1:15	4	Q15672
427	UBAC1	UBAC1	UBAC1	10422	Sigma-Aldrich	HPA005651	1270	AB_1858489	Rabbit	Valid	1:250	-20	Q9BSL1
428	UGT1A	UGT1A	UGT1A1	54658	Santa Cruz	sc-271268	1267	AB_10610640	Mouse	Valid	1:50	4	P22309
429	ULK1 (phospho Ser757)	ULK1_pS757	ULK1	8408	CST	6888	1626	AB_10829226	Rabbit	Caution	1:200	-20	O75385
430	VASP	VASP	VASP	7408	CST	3112	678	AB_2213542	Rabbit	Valid	1:100	-20	P50552
431	Vav1	Vav1	VAV1	4709	CST	2502	1181	AB_2213556	Rabbit	Caution	1:75	-20	P15498
432	VEGF Receptor 2	VEGFR-2	KDR	3791	CST	2479	688	AB_2212507	Rabbit	Valid	1:5000	-20	P35968
433	VEGF Receptor 2 (phospho Tyr1175)	VEGFR2_pY1175	KDR	3791	CST	3770	2416	AB_1642326	Rabbit	Caution	1:300	-20	P35968
434	VHL	VHL	VHL	7428	CST	68547	2394	AB_2716279	Rabbit	Caution	1:250	-20	P40337
435	VHL/EPPK1†	VHL-EPPK1	VHL-EPPK1	83481	BD Biosciences	556347	693	AB_396376	Mouse	Caution	1:7500	4	P58107
436	Vinculin	Vinculin	VCL	7414	Sigma-Aldrich	SAB4200080	1343	AB_10604160	Mouse	Valid	1:75000	-20	P18206
437	Wee1	Wee1	WEE1	7465	CST	4936	1802	AB_2288509	Rabbit	Caution	1:150	-20	P30291
438	Wee1 (phospho Ser642)	Wee1_pS642	WEE1	7465	CST	4910	2058	AB_2215870	Rabbit	Caution	1:25	-20	P30291
439	WIPI1	WIPI1	WIPI1	55062	CST	12124	1627	AB_2750868	Rabbit	Caution	1:50	-20	Q5MNZ9
440	WIPI2	WIPI2	WIPI2	26100	CST	8567	1628	AB_11178945	Rabbit	Caution	1:75	-20	Q9Y4P8
441	WTAP	WTAP	WTAP	9589	Abcam	ab195380	2576	AB_2868572	Rabbit	Valid	1:750	-20	Q15007
442	XBP-1 [discontinued]	XBP-1	XBP1	7494	Santa Cruz	sc-32136	1044	AB_2304558	Goat	Caution	1:200	4	P17861
443	XIAP	XIAP	XIAP	331	CST	2042	699	AB_2214870	Rabbit	Caution	1:30	-20	P98170
444	XPA	XPA	XPA	7507	Santa Cruz	sc-56813	1359	AB_794177	Mouse	Valid	1:75	4	P23025
445	XPF	XPF	ERCC4	2072	CST	13465	2525	AB_2798227	Rabbit	Caution	1:100	-20	Q92889
446	XRCC1	XRCC1	XRCC1	7515	Invitrogen	PA5-29359	2440	AB_2546835	Rabbit	Caution	1:250	-20	P18887
447	YAP (phospho Ser127)	YAP_pS127	YAP1	10413	CST	4911	782	AB_2218913	Rabbit	Valid	1:1000	-20	P46937
448	YAP [discontinued]	YAP	YAP1	10413	Santa Cruz	sc-15407	780	AB_2273277	Rabbit	Caution	1:750	4	P46937
449	YB1 (phospho Ser102)	YB1_pS102	YBX1	4904	CST	2900	835	AB_2219273	Rabbit	Valid	1:50	-20	P67809
450	YES1	YES1	YES1	7525	CST	3201	2499	AB_11178531	Rabbit	Valid	1:50	-20	P07947
451	YTHDF2	YTHDF2	YTHDF2	51441	Abcam	ab220163	2579	AB_2868573	Rabbit	Valid	1:10000	-20	Q9Y5A9
452	YTHDF3	YTHDF3	YTHDF3	253943	Abcam	ab220161	2572	AB_2868574	Rabbit	Caution	1:750	-20	Q7Z739
453	ZAP-70	ZAP-70	ZAP70	7535	CST	3165	2555	AB_2218656	Rabbit	Valid	1:500	-20	P43403

**RPPA Core Ab Name: Antibody names listed were created by the RPPA Core for general reference purposes only and are not to be used as official UniProt names for publishing purposes.

†VHL/EPPK1 Antibody has been identified to recognize EPPK1 by MS, RNAi, and correlation with RNA expression

Standard Antibody List: Set 147 to Present

Note: VHL Antibody has been identified to recognize EPPK1 by MS, RNAi and correlation with RNA expression

#	Official Ab Name	Ab Name Reported on Dataset	Gene Name	Company	Catalog #	Internal Ab ID	Species	Validation Status*	RPPA Dilution	Storage
1	14-3-3 beta	14-3-3-beta	YWHAB	Santa Cruz	sc-628	882	Rabbit	Valid	1:75	4
2	14-3-3 zeta	14-3-3-zeta	YWHAZ	Santa Cruz	sc-1019	883	Rabbit	Valid	1:5000	4
3	4E-BP1	4E-BP1	EIF4EBP1	CST	9452	2	Rabbit	Valid	1:100	-20
4	4E-BP1 (phospho S65)	4E-BP1_pS65	EIF4EBP1	CST	9456	3	Rabbit	Valid	1:250	-20
5	53BP1	53BP1	TP53BP1	CST	4937	985	Rabbit	Valid	1:300	-20
6	Acetyl CoA Carboxylase (phospho S79)	ACC_pS79	ACACA, B	CST	3661	13	Rabbit	Valid	1:500	-20
7	Acetyl CoA Carboxylase 1	ACC1	ACACA	Abcam	ab45174	14	Rabbit	Use with Caution	1:20000	-20
8	ADAR1	ADAR1	ADAR	Abcam	ab88574	1198	Mouse	Valid	1:200	-20
9	Akt	Akt	AKT1, 2, 3	CST	4691	1084	Rabbit	Valid	1:10000	-20
10	Akt (phospho S473)	Akt_pS473	AKT1, 2, 3	CST	9271	230	Rabbit	Valid	1:150	-20
11	Akt (phospho T308)	Akt_pT308	AKT1, 2, 3	CST	2965	1154	Rabbit	Valid	1:500	-20
12	AMPK alpha	AMPKa	PRKAA1	CST	2532	39	Rabbit	Use with Caution	1:200	-20
13	AMPK alpha (phospho T172)	AMPKa_pT172	PRKAA1	CST	2535	40	Rabbit	Use with Caution	1:100	-20
14	AMPK alpha 2 (Phospho S345)	AMPK-a2_pS345	PRKAA2	Abcam	ab129081	1351	Rabbit	Valid	1:500	-20
15	Androgen Receptor	AR	AR	CST	5153	2191	Rabbit	Valid	1:250	-20
16	Annexin I	Annexin-I	ANXA1	BD Biosciences	610066	1208	Mouse	Valid	1:5000	-20
17	Annexin VII	Annexin-VII	ANXA7	BD Biosciences	610668	1142	Mouse	Valid	1:30	-20
18	A-Raf	A-Raf	ARAF	CST	4432	1217	Rabbit	Valid	1:150	-20
19	ARID1A	ARID1A	ARID1A	Sigma-Aldrich	HPA005456	1442	Rabbit	Use with Caution	1:1000	-20
20	Atg3	Atg3	ATG3	CST	3415	1612	Rabbit	Valid	1:750	-20
21	Atg7	Atg7	ATG7	CST	8558	1613	Rabbit	Valid	1:1000	-20
22	ATM	ATM	ATM	CST	2873	1363	Rabbit	Valid	1:250	-20
23	ATM (phospho S1981)	ATM_pS1981	ATM	CST	5883	1364	Rabbit	Valid	1:25	-20
24	ATR (Phospho S428)	ATR_pS428	ATR	Abcam	ab178407	1795	Rabbit	Use with Caution	1:1000	-20
25	Aurora B/AIM1	Aurora-B	AIM1	CST	3094	1404	Rabbit	Valid	1:50	-20
26	Axl	Axl	AXL	CST	8661	1271	Rabbit	Valid	1:1000	-20
27	B7-H4	B7-H4	VTCN1	CST	14572	1726	Rabbit	Use with Caution	1:50	-20
28	Bad (phospho S112)	Bad_pS112	BAD	CST	9291	63	Rabbit	Valid	1:50	-20
29	Bak	Bak	BAK1	Abcam	ab32371	71	Rabbit	Use with Caution	1:30	-20
30	BAP1	BAP1	BAP1	Santa Cruz	sc-28383	1207	Mouse	Valid	1:125	4
31	Bax	Bax	BAX	CST	2772	73	Rabbit	Valid	1:100	-20
32	Bcl2	Bcl2	BCL2	Dako	M0887	80	Mouse	Valid	1:50	4
33	Bcl-xL	Bcl-xL	BCL2L1	CST	2762	85	Rabbit	Valid	1:100	-20
34	Beclin	Beclin	BECN1	ThermoFisher	PA1-16857	2269	Rabbit	Use with Caution	1:500	4
35	beta Actin	b-Actin	ACTB	CST	4970	1169	Rabbit	Use with Caution	1:75	-20
36	beta Catenin	b-Catenin	CTNNB1	CST	9562	75	Rabbit	Valid	1:1500	-20
37	beta Catenin (phospho T41/S45)	b-Catenin_pT41_S45	CTNNB1	CST	9565	1170	Rabbit	Valid	1:30	-20
38	Bid	Bid	BID	CST	2002	89	Rabbit	Use with Caution	1:1000	-20
39	Bim	Bim	BCL2L11	Abcam	ab32158	90	Rabbit	Valid	1:400	-20
40	BiP/GRP78	BiP-GRP78	HSPA5	BD Biosciences	610978	1311	Mouse	Use with Caution	1:750	-20
41	B-Raf	B-Raf	BRAF	CST	14814	2083	Rabbit	Use with Caution	1:500	-20
42	B-Raf (phospho S445)	B-Raf_pS445	BRAF	CST	2696	94	Rabbit	Valid	1:1000	-20
43	BRD4	BRD4	BRD4	CST	13440	1567	Rabbit	Valid	1:200	-20
44	c-Abl	c-Abl	ABL	CST	2862	1565	Rabbit	Valid	1:50	-20
45	c-IAP2	c-IAP2	BIRC3	CST	3130	1615	Rabbit	Use with Caution	1:750	-20
46	Caspase-3 active	Caspase-3	CASP3	Abcam	ab32042	108	Rabbit	Use with Caution	1:250	-20
47	Caspase-7 (cleaved D198)	Caspase-7-cleaved	CASP7	CST	9491	109	Rabbit	Use with Caution	1:75	-20
48	Caspase-8	Caspase-8	CASP8	CST	9746	951	Mouse	Used for QC	1:150	-20
49	Caveolin-1	Caveolin-1	CAV1	CST	3238	114	Rabbit	Valid	1:5000	-20
50	CD4	CD4	CD4	Abcam	ab133616	2104	Rabbit	Valid	1:500	-20
51	CD20	CD20	MS4A1	Abcam	ab78237	125	Rabbit	Use with Caution	1:75	-20
52	CD29	CD29	ITGB1	BD Biosciences	610467	1206	Mouse	Valid	1:30	-20
53	CD31	CD31	PECAM1	Dako	M0823	127	Mouse	Valid	1:30	4
54	CD44	CD44	CD44	CST	3570	1398	Mouse	Use with Caution	1:50	-20
55	CD45	CD45	CD45	DAKO	M070129-2	1352	Mouse	Valid	1:1000	4
56	CD49b	CD49b	ITGA2	BD Biosciences	611016	937	Mouse	Valid	1:50	-20
57	CD134/OX40	CD134	CD134	Abcam	ab76000	2100	Rabbit	Valid	1:100	-20
58	cdc2 (Phospho Y15)	cdc2_pY15	CDK1	CST	4539	1783	Rabbit	Use with Caution	1:250	-20
59	cdc25C	cdc25C	CDC25C	CST	4688	1873	Rabbit	Valid	1:500	-20
60	CDK1	CDK1	CDK1	Abcam	ab32384	1658	Rabbit	Use with Caution	1:1000	-20
61	CDKN2A/p16INK4a	p16INK4a	CDKN2A	Abcam	ab81278	1231	Rabbit	Valid	1:500	-20
62	Chk1	Chk1	CHEK1	CST	2360	1203	Mouse	Use with Caution	1:250	-20
63	Chk1 (phospho S296)	Chk1_pS296	CHEK1	Abcam	ab79758	1348	Rabbit	Valid	1:125	-20
64	Chk2	Chk2	CHEK2	CST	3440	146	Mouse	Valid	1:50	-20
65	Chk2 (phospho T68)	Chk2_pT68	CHEK2	CST	2197	147	Rabbit	Use with Caution	1:125	-20
66	c-Jun ( phospho S73)	c-Jun_pS73	JUN	CST	9164	155	Rabbit	Valid	1:30	-20
67	c-Kit	c-Kit	KIT	Abcam	ab32363	157	Rabbit	Valid	1:30	-20
68	Claudin 7	Claudin-7	CLDN7	Novus Biologicals	NB100-91714	852	Rabbit	Valid	1:300	-20
69	c-Met (phospho Y1234/Y1235)	c-Met_pY1234_Y1235	MET	CST	3129	727	Rabbit	Valid	1:100	-20
70	c-Myc	c-Myc	MYC	Santa Cruz	sc-764	1143	Rabbit	Use with Caution	1:125	4
71	COG3	COG3	COG3	ProteinTech	11130-1-AP	1656	Rabbit	Valid	1:750	-20
72	COL6A1	Collagen-VI	COL6A1	Santa Cruz	sc-20649	171	Rabbit	Valid	1:5000	4
73	Connexin 43	Connexin-43	CNST43	CST	3512	1568	Rabbit	Use with Caution	1:150	-20
74	Cox2	Cox2	PTGS2	CST	4842	1218	Rabbit	Use with Caution	1:50	-20
75	Cox-IV	Cox-IV	COX4I1	CST	4850	1116	Rabbit	Valid	1:1000	-20
76	C-Raf (phospho S338)	C-Raf_pS338	RAF1	CST	9427	179	Rabbit	Valid	1:100	-20
77	C-Raf/Raf-1	C-Raf	RAF1	Millipore	04-739	1201	Rabbit	Use with Caution	1:200	-20
78	CREB	CREB	CREB1	CST	9197	181	Rabbit	Use with Caution	1:1000	-20
79	Cyclin B1	Cyclin-B1	CCNB1	Epitomics	1495-1	192	Rabbit	Valid	1:1500	-20
80	Cyclin D1	Cyclin-D1	CCND1	Santa Cruz	sc-718	194	Rabbit	Valid	1:200	4
81	Cyclin D3	Cyclin-D3	CCND3	CST	2936	198	Mouse	Valid	1:1000	-20
82	Cyclin E1	Cyclin-E1	CCNE1	Santa Cruz	sc-247	201	Mouse	Valid	1:30	4
83	Cyclophilin F	Cyclophilin-F	PPIF	Abcam	ab110324	1257	Mouse	Valid	1:50000	4
84	Detyrosinated alpha-Tubulin	D-a-Tubulin	TUBA4A, TUBA3C	Abcam	ab48389	1379	Rabbit	Valid	1:500	-20
85	Dimethyl-Histone H3 (Lys4)	DM-Histone-H3	HIST1H3A	CST	9725	2290	Rabbit	Valid	1:100	-20
86	Dimethyl-K9 Histone H3	DM-K9-Histone-H3	HIST3H3	Abcam	ab1220	2319	Mouse	Valid	1:250	-20
87	DUSP4/MKP2	DUSP4	DUSP4	CST	5149	1406	Rabbit	Valid	1:250	-20
88	E2F-1	E2F1	E2F1	Santa Cruz	sc-251	1261	Mouse	Valid	1:30	4
89	E-Cadherin	E-Cadherin	CDH1	CST	3195	1099	Rabbit	Valid	1:300	-20
90	eEF2	eEF2	EEF2	CST	2332	1060	Rabbit	Use with Caution	1:50	-20
91	eEF2K	eEF2K	EEF2K	CST	3692	1061	Rabbit	Valid	1:50	-20
92	EGFR	EGFR	EGFR	CST	2232	1120	Rabbit	Valid	1:100	-20
93	EGFR (phospho Y1173)	EGFR_pY1173	EGFR	Abcam	ab32578	221	Rabbit	Valid	1:50	-20
94	eIF4E	eIF4E	EIF4E	CST	9742	722	Rabbit	Valid	1:75	-20
95	eIF4E (Phospho S209)	eIF4E_pS209	EIF4E	Abcam	ab76256	1871	Rabbit	Valid	1:500	-20
96	eIF4G	eIF4G	EIF4G1	CST	2498	1124	Rabbit	Use with Caution	1:1000	-20
97	Elk1 (phospho S383)	Elk1_pS383	ELK1	CST	9181	228	Rabbit	Use with Caution	1:50	-20
98	ENY2	ENY2	ENY2	GeneTex	GTX629542	1219	Mouse	Use with Caution	1:1000	-20
99	Epithelial Membrane Antigen	EMA	EMA	Dako	M061329-2	1350	Mouse	Use with Caution	1:1000	4
100	ErbB2/HER2	HER2	ERBB2	Lab Vision	MS-325-P1	1038	Mouse	Valid	1:3000	4
101	ErbB2/HER2 (phospho Y1248)	HER2_pY1248	ERBB2	R&D Systems	AF1768	1075	Rabbit	Use with Caution (likely sees pEGFR)	1:1500	-20
102	ErbB3/HER3	HER3	ERBB3	Santa Cruz	sc-285	911	Rabbit	Valid	1:300	4
103	ErbB3/HER3 (phospho Y1289)	HER3_pY1289	ERBB3	CST	4791	728	Rabbit	Use with Caution	1:50	-20
104	ERCC1	ERCC1	ERCC1	Santa Cruz	sc-17809	1357	Mouse	Valid	1:30	4
105	ERCC5	ERCC5	ERCC5	ProteinTech	11331-1-AP	1355	Rabbit	Use with Caution	1:250	-20
106	ERRFI1/MIG6	MIG6	ERRFI1	Sigma-Aldrich	WH0054206M1	1062	Mouse	Valid	1:50	-20
107	Estrogen Receptor	ER	ESR1	Lab Vision	RM-9101	238	Rabbit	Valid	1:40	4
108	Estrogen Receptor alpha (Phospho S118)	ER-a_pS118	ESR1	Abcam	ab32396	241	Rabbit	Valid	1:1000	-20
109	Ets-1	Ets-1	ETS1	Bethyl	A303-501A	1200	Rabbit	Valid	1:100	4
110	FAK	FAK	PTK2	Abcam	ab40794	252	Rabbit	Use with Caution	1:1000	-20
111	FAK (phospho Y397)	FAK_pY397	PTK2	CST	3283	1227	Rabbit	Valid	1:30	-20
112	Fatty Acid Synthase	FASN	FASN	CST	3180	1156	Rabbit	Valid	1:1000	-20
113	Fibronectin	Fibronectin	FN1	Epitomics	1574-1	262	Rabbit	Valid	1:10000	-20
114	FoxM1	FoxM1	FOXM1	CST	5436	1123	Rabbit	Valid	1:30	-20
115	FoxO3a	FoxO3a	FOXO3	CST	2497	1122	Rabbit	Use with Caution	1:25	-20
116	FoxO3a (phospho S318/S321)	FoxO3a_pS318_S321	FOXO3	CST	9465	270	Rabbit	Use with Caution	1:30	-20
117	G6PD	G6PD	G6PD	CST	8866	1779	Rabbit	Valid	1:1000	-20
118	Gab2	Gab2	GAB2	CST	3239	943	Rabbit	Valid	1:300	-20
119	GAPDH	GAPDH	GAPDH	Life Technologies	AM4300	274	Mouse	Use with Caution	1:50000	-20
120	GATA3	GATA3	GATA3	BD Biosciences	558686	764	Mouse	Valid	1:300	4
121	GATA6	GATA6	GATA6	CST	5851	2309	Rabbit	Valid	1:200	-20
122	GCLM	GCLM	GCLM	Abcam	ab124827	1745	Rabbit	Use with Caution	1:500	-20
123	GCN5L2	GCN5L2	KAT2A	CST	3305	1263	Rabbit	Valid	1:30	-20
124	Glutamate Dehydrogenase1/2	Glutamate-D1-2	GLUD	Novus Biologicals	NBP2-16679	2320	Rabbit	Valid	1:500	-20
125	Glutaminase	Glutaminase	GLS	Abcam	ab156876	1491	Rabbit	Use with Caution	1:250	-20
126	Glycogen Synthase	Gys	GYS1	CST	3886	1035	Rabbit	Valid	1:1500	-20
127	Glycogen Synthase (phospho S641)	Gys_pS641	GYS1	CST	3891	1036	Rabbit	Valid	1:250	-20
128	Granzyme B	Granzyme-B	GZMB	CST	4275	1807	Rabbit	Valid	1:500	-20
129	GSK-3alpha/beta	GSK-3a-b	GSK3A, B	Santa Cruz	sc-7291	284	Mouse	Valid	1:750	4
130	GSK-3alpha/beta (phospho S21/S9)	GSK-3a-b_pS21_S9	GSK3A, B	CST	9331	285	Rabbit	Valid	1:200	-20
131	H2AX (phospho S140)	H2AX_pS140	H2AX	Pierce Biotechnology	MA1-2022	1409	Mouse	Use with Caution	1:400	-20
132	Heregulin	Heregulin	NRG1	CST	2573	890	Rabbit	Valid	1:30	-20
133	HES1	HES1	HES1	CST	11988	1582	Rabbit	Valid	1:1000	-20
134	Hexokinase II	Hexokinase-II	HK2	CST	2106	2293	Rabbit	Valid	1:100	-20
135	Hif-1 alpha	Hif-1-alpha	HIF1A	BD Biosciences	610958	1402	Mouse	Use with Caution	1:50	-20
136	Histone H3	Histone-H3	H3F3A, B	Abcam	ab1791	1250	Rabbit	Valid	1:3000	-20
137	HLA-DR/DP/DQ/DX	HLA-DR-DP-DQ-DX	HLA	Santa Cruz	sc-53302	2016	Mouse	Valid	1:1000	4
138	HSP27	HSP27	HSP27	CST	2402	321	Mouse	Use with Caution	1:100	-20
139	HSP27 (phospho S82)	HSP27_pS82	HSBP1	CST	2401	323	Rabbit	Valid	1:75	-20
140	HSP70	HSP70	HSP70	CST	4872	325	Rabbit	Use with Caution	1:150	-20
141	IGF1R (phospho Y1135/Y1136)	IGF1R_pY1135_Y1136	IGF1R	CST	3024	1221	Rabbit	Valid	1:30	-20
142	IGFBP2	IGFBP2	IGFBP2	CST	3922	335	Rabbit	Valid	1:50	-20
143	IGFRb	IGFRb	INSR	CST	3027	336	Rabbit	Use with Caution	1:250	-20
144	INPP4b	INPP4b	INPP4B	CST	4039	1065	Rabbit	Valid	1:25	-20
145	Insulin Receptor beta	IR-b	INSRB	CST	3025	1586	Rabbit	Use with Caution	1:750	-20
146	IRF-1	IRF-1	IRF1	Santa Cruz	sc-497	1316	Rabbit	Use with Caution	1:200	4
147	IRS1	IRS1	IRS1	Millipore	06-248	802	Rabbit	Valid	1:400	-20
148	Jagged1	Jagged1	JAG1	Abcam	ab109536	1413	Rabbit	Valid	1:750	-20
149	Jak2	Jak2	JAK2	CST	3230	1166	Rabbit	Valid	1:750	-20
150	JNK/SAPK (phospho T183/Y185)	JNK_pT183_Y185	MAPK8	CST	4668	888	Rabbit	Valid	1:30	-20
151	JNK2	JNK2	MAPK9	CST	4672	380	Rabbit	Use with Caution	1:30	-20
152	LC3A/B	LC3A-B	LC3AB	CST	4108	1618	Rabbit	Use with Caution	1:500	-20
153	Lck	Lck	LCK	CST	2752	397	Rabbit	Valid	1:100	-20
154	LDHA	LDHA	LDHA	CST	3582	976	Rabbit	Use with Caution	1:250	-20
155	LRP6 (phospho S1490)	LRP6_pS1490	LRP6	CST	2568	1367	Rabbit	Valid	1:500	-20
156	MAPK (phospho T202/Y204)	MAPK_pT202_Y204	MAPK1, 3	CST	4377	405	Rabbit	Valid	1:30	-20
157	Mcl 1	Mcl-1	MCL1	CST	5453	1222	Rabbit	Valid	1:100	-20
158	MDM2 (phospho S166)	MDM2_pS166	MDM2	CST	3521	1164	Rabbit	Valid	1:50	-20
159	MEK1	MEK1	MAP2K1	Abcam	ab32576	417	Rabbit	Valid	1:1500	-20
160	MEK1 (phospho S217/S221)	MEK1_pS217_S221	MAP2K1, 2	CST	9154	1076	Rabbit	Valid	1:50	-20
161	MERIT40 (Phospho S29)	MERIT40_pS29	BABAM1	CST	12110	1952	Rabbit	Valid	1:1000	-20
162	Merlin/NF2	Merlin	NF2	Novus Biologicals	22710002	1046	Rabbit	Use with Caution	1:250	-20
163	MIF	MIF	MIF	Santa Cruz	sc-20121	1323	Rabbit	Use with Caution	1:300	4
164	MMP2	MMP2	MMP2	CST	4022	435	Rabbit	Valid	1:75	-20
165	Mnk1	Mnk1	MKNK1	CST	2195	1005	Rabbit	Valid	1:1000	-20
166	Monocarboxylic Acid Transporter 4	MCT4	MCT3	Millipore	AB3314P	1633	Rabbit	Valid	1:500	-20
167	MSH6	MSH6	MSH6	Novus Biologicals	22030002	1063	Rabbit	Use with Caution	1:1000	-20
168	MSI2	MSI2	MSI2	Abcam	ab76148	1675	Rabbit	Use with Caution	1:4000	-20
169	mTOR	mTOR	MTOR	CST	2983	444	Rabbit	Valid	1:1000	-20
170	mTOR (phospho S2448)	mTOR_pS2448	MTOR	CST	2971	446	Rabbit	Use with Caution	1:50	-20
171	Myosin heavy chain 11	Myosin-11	MYH11	Novus Biologicals	21370002	1139	Rabbit	Valid	1:5000	-20
172	Myosin IIa (phospho S1943)	Myosin-IIa_pS1943	MYH9	CST	5026	1160	Rabbit	Valid	1:1000	-20
173	Myt1	Myt1	MYT1	CST	4282	1803	Rabbit	Use with Caution	1:2000	-20
174	NAPSIN A	NAPSIN-A	NAPSA	Abcam	ab129189	1274	Rabbit	Use with Caution	1:150	-20
175	N-Cadherin	N-Cadherin	CDH2	CST	4061	452	Rabbit	Valid	1:30	-20
176	NDRG1 (phospho T346)	NDRG1_pT346	NDRG1	CST	3217	1126	Rabbit	Valid	1:100	-20
177	NDUFB4	NDUFB4	NDUFB4	Abcam	ab110243	1345	Mouse	Valid	1:30	4
178	NF-kappaB p65 (phospho S536)	NF-kB-p65_pS536	RELA	CST	3033	457	Rabbit	Use with Caution	1:30	-20
179	Notch1	Notch1	NOTCH1	CST	3268	1064	Rabbit	Valid	1:30	-20
180	Notch3	Notch3	NOTCH3	Santa Cruz	sc-5593	767	Rabbit	Use with Caution	1:300	4
181	N-Ras	N-Ras	NRAS	Santa Cruz	sc-31	1136	Mouse	Valid	1:50	4
182	Oct-4	Oct-4	OCT4	CST	2750	1669	Rabbit	Use with Caution	1:200	-20
183	p21	p21	CDKN1A	Invitrogen	MA5-14949	2316	Rabbit	Use with Caution	1:500	-20
184	p27 KIP 1	p27-Kip-1	CDKN1B	Abcam	ab32034	897	Rabbit	Valid	1:500	-20
185	p27/KIP 1 (phospho T198)	p27_pT198	CDKN1B	Abcam	ab64949	878	Rabbit	Valid	1:30	-20
186	p38 MAPK	p38	MAPK14	CST	9212	478	Rabbit	Valid	1:1500	-20
187	p38 MAPK (phospho T180/Y182)	p38_pT180_Y182	MAPK14	CST	9211	479	Rabbit	Valid	1:50	-20
188	p44/42 MAPK	p44-42-MAPK	MAPK3	CST	4695	1119	Rabbit	Valid	1:2000	-20
189	p53	p53	TP53	CST	9282	481	Rabbit	Use with Caution	1:2500	-20
190	p70 S6 Kinase (phospho T389)	p70-S6K_pT389	RPS6KB1	CST	9205	494	Rabbit	Valid	1:50	-20
191	p70/S6K1	p70-S6K1	RPS6KB1	Abcam	ab32529	493	Rabbit	Valid	1:300	-20
192	p90RSK (phospho T573)	p90RSK_pT573	RPS6K	CST	9346	1178	Rabbit	Use with Caution	1:30	-20
193	PAI-1	PAI-1	SERPINE1	BD Biosciences	612024	499	Mouse	Valid	1:100	-20
194	PAICS	PAICS	PAICS	Sigma-Aldrich	HPA035895	1322	Rabbit	Use with Caution	1:250	-20
195	PAK1	PAK1	PAK1	CST	2602	1811	Rabbit	Valid	1:1000	-20
196	PAK4	PAK4	PAK4	CST	3242	1389	Rabbit	Valid	1:750	-20
197	PAR	PAR	PAR	Trevigen	4336-BPC-100	1370	Rabbit	Use with Caution	1:15000	-20
198	PARK7/DJ1	DJ1	PARK7	Abcam	ab76008	891	Rabbit	Valid	1:5000	-20
199	PARP	PARP	PARP1	CST	9532	2185	Rabbit	Valid	1:1000	-20
200	Paxillin	Paxillin	PXN	CST	2542	504	Rabbit	Use with Caution	1:250	-20
201	P-Cadherin	P-Cadherin	CDH3	CST	2130	509	Rabbit	Use with Caution	1:50	-20
202	PCNA	PCNA	PCNA	CST	2586	1383	Mouse	Use with Caution	1:1000	-20
203	PD-1	PD-1	PD1	CST	43248	2095	Rabbit	Valid	1:500	-20
204	Pdcd4	Pdcd4	PDCD4	Rockland	600-401-965	816	Rabbit	Use with Caution	1:750	-20
205	PDGFR beta	PDGFR-b	PDGFRB	Invitrogen	MA5-15143	2318	Rabbit	Valid	1:500	-20
206	PDHK1	PDHK1	PDHK1	CST	3820	1622	Rabbit	Use with Caution	1:500	-20
207	PDK1	PDK1	PDPK1	CST	3062	515	Rabbit	Valid	1:50	-20
208	PDK1 (phospho S241)	PDK1_pS241	PDPK1	CST	3061	516	Rabbit	Valid	1:50	-20
209	PD-L1	PD-L1	CD274	CST	13684	1724	Rabbit	Use with Caution	1:150	-20
210	PEA-15	PEA-15	PEA15	CST	2780	1017	Rabbit	Valid	1:100	-20
211	PED/PEA-15 (phospho S116)	PEA-15_pS116	PEA15	Invitrogen	44-836G	1018	Rabbit	Valid	1:1000	-20
212	PI3 Kinase p110 alpha	PI3K-p110-a	PIK3CA	CST	4255	808	Rabbit	Use with Caution	1:75	-20
213	PI3K p110 beta	PI3K-p110-b	PIK3CB	Santa Cruz	sc-376412	1330	Mouse	Use with Caution	1:60	4
214	PI3K p85	PI3K-p85	PIK3R1	Millipore	06-195	523	Rabbit	Valid	1:15000	-20
215	PKA RI alpha	PKA-a	PRKAR1A	CST	5675	1667	Rabbit	Valid	1:200	-20
216	PKCalpha	PKCa	PRKCA	CST	2056	1158	Rabbit	Valid	1:200	-20
217	PKC beta II (phospho S660)	PKC-b-II_pS660	PRKCA, B, D, E, H, Q	CST	9371	1137	Rabbit	Valid	1:200	-20
218	PKC delta (phospho S664)	PKC-delta_pS664	PRKCD	Millipore	07-875	932	Rabbit	Valid	1:100	-20
219	PKM2	PKM2	PKM2	CST	4053	1025	Rabbit	Use with Caution	1:300	-20
220	PLC gamma2 (phospho Y759)	PLC-gamma2_pY759	PLCG2	CST	3874	1030	Rabbit	Use with Caution	1:30	-20
221	PLK1	PLK1	PLK1	CST	4513	754	Rabbit	Use with Caution	1:125	-20
222	PMS2	PMS2	PMS2	Novus Biologicals	22510002	1246	Rabbit	Valid	1:1500	-20
223	PRAS40	PRAS40	AKT1S1	Invitrogen	AHO1031	738	Mouse	Use with Caution	1:250	-20
224	PRAS40 (phospho T246)	PRAS40_pT246	AKT1S1	Life Technologies	441100G	739	Rabbit	Valid	1:500	-20
225	PREX1	PREX1	PREX1	Abcam	ab102739	1204	Rabbit	Valid	1:150	-20
226	Progesterone Repector	PR	PGR	Abcam	ab206926	2296	Rabbit	Valid	1:500	-20
227	PTEN	PTEN	PTEN	CST	9552	566	Rabbit	Valid	1:500	-20
228	Rab11	Rab11	RAB11A, B	CST	3539	1083	Rabbit	Use with Caution	1:30	-20
229	Rab25	Rab25	RAB25	CST	4314	1150	Rabbit	Valid	1:30	-20
230	Rad50	Rad50	RAD50	CST	3427	1454	Rabbit	Valid	1:250	-20
231	Rad51	Rad51	RAD51	Millipore	ABE257	2112	Rabbit	Use with Caution	1:1000	4
232	Raptor	Raptor	RPTOR	CST	2280	1128	Rabbit	Valid	1:300	-20
233	Rb	Rb	RB1	CST	9309	552	Mouse	Used for QC	1:100	-20
234	Rb (phospho S807/S811)	Rb_pS807_S811	RB1	CST	9308	557	Rabbit	Valid	1:500	-20
235	RBM15	RBM15	RBM15	Novus Biologicals	21390002	1138	Rabbit	Valid	1:5000	-20
236	Rheb	Rheb	RHEB	R&D Systems	MAB3426	847	Mouse	Use with Caution	1:75	-20
237	Rictor	Rictor	RICTOR	CST	2114	1129	Rabbit	Use with Caution	1:100	-20
238	Rictor (phospho T1135)	Rictor_pT1135	RICTOR	CST	3806	1130	Rabbit	Valid	1:200	-20
239	RIP	RIP	RIP	CST	4926	1624	Rabbit	Use with Caution	1:250	-20
240	RPA32	RPA32	RPA32	CST	2208	1368	Rat	Use with Caution	1:500	-20
241	RPA32 (Phospho S4/S8)	RPA32_pS4_S8	RPA32	Bethyl	A300-245A	1375	Rabbit	Use with Caution	1:250	4
242	RSK	RSK	RPS6KA1, 2, 3	CST	9347	759	Rabbit	Use with Caution	1:150	-20
243	S6 (phospho S235/S236)	S6_pS235_S236	RPS6	CST	2211	600	Rabbit	Valid	1:2500	-20
244	S6 (phospho S240/S244)	S6_pS240_S244	RPS6	CST	2215	601	Rabbit	Valid	1:1000	-20
245	S6 Ribosomal Protein	S6	RPS6	CST	2317	1874	Mouse	Valid	1:1000	-20
246	SCD	SCD	SCD	Santa Cruz	sc-58420	1127	Mouse	Valid	1:30	4
247	SDHA	SDHA	SDHA	CST	11998	1339	Rabbit	Valid	1:250	-20
248	SF2/ASF	SF2	SRSF1	Invitrogen	32-4500	1131	Mouse	Valid	1:150	-20
249	Shc (phospho Y317)	Shc_pY317	SHC1	CST	2431	1031	Rabbit	Valid	1:30	-20
250	SHP-2 (phospho Y542)	SHP-2_pY542	PTPN11	CST	3751	1180	Rabbit	Use with Caution	1:75	-20
251	SLC1A5	SLC1A5	SLC1A5	Sigma-Aldrich	HPA035240	1313	Rabbit	Use with Caution	1:15000	-20
252	Slfn11	Slfn11	SLFN11	Santa Cruz	sc-136891	1411	Goat	Use with Caution	1:250	4
253	Smac/Diablo	Smac	DIABLO	CST	2954	610	Mouse	Used for QC	1:150	-20
254	Smad1	Smad1	SMAD1	Abcam	ab33902	922	Rabbit	Valid	1:750	-20
255	Smad3	Smad3	SMAD3	Abcam	ab40854	796	Rabbit	Valid	1:150	-20
256	Smad4	Smad4	SMAD4	Santa Cruz	sc-7966	920	Mouse	Valid	1:30	4
257	Snail	Snail	SNAI1	CST	3895	616	Mouse	Used for QC	1:50	-20
258	SOD1	SOD1	SOD1	CST	4266	1818	Mouse	Valid	1:500	-20
259	SOD2	SOD2	SOD2	CST	13194	2291	Rabbit	Valid	1:200	-20
260	Sox2	Sox2	SOX2	CST	2748	1670	Rabbit	Valid	1:200	-20
261	Src	Src	SRC	Millipore	05-184	621	Mouse	Valid	1:200	-20
262	Src (phospho Y527)	Src_pY527	SRC, YES1, FYN, FGR	CST	2105	626	Rabbit	Valid	1:30	-20
263	Src Family (phospho Y416)	Src_pY416	SRC, LYN, FYN, LCK, YES1, HCK	CST	2101	623	Rabbit	Valid	1:500	-20
264	Stat3	Stat3	STAT3	CST	4904	1197	Rabbit	Use with Caution	1:3000	-20
265	Stat3 (phospho Y705)	Stat3_pY705	STAT3	CST	9145	2101	Rabbit	Valid	1:100	-20
266	Stat5a	Stat5a	STAT5A	Abcam	ab32043	638	Rabbit	Valid	1:250	-20
267	Stathmin 1	Stathmin-1	STMN1	Abcam	ab52630	718	Rabbit	Valid	1:75	-20
268	Sting	STING	STING	CST	13647	2099	Rabbit	Valid	1:250	-20
269	Syk	Syk	SYK	Santa Cruz	sc-1240	1033	Mouse	Valid	1:3000	4
270	Tau	Tau	TAU	Millipore	05-348	646	Mouse	Use with Caution	1:100	-20
271	TAZ	TAZ	WWTR1	CST	4883	1848	Rabbit	Valid	1:300	-20
272	TFAM	TFAM	TFAM	CST	7495	1333	Rabbit	Valid	1:300	-20
273	TIGAR	TIGAR	C12ORF5	Abcam	ab137573	1107	Rabbit	Valid	1:100	-20
274	Transferrin Receptor	TFRC	TFRC	Novus Biologicals	22500002	1140	Rabbit	Valid	1:15000	-20
275	Transglutaminase II	Transglutaminase	TGM2	Lab Vision	MS-224-P1	908	Mouse	Valid	1:150	4
276	TRIM25	TRIM25	TRIM25	Abcam	ab167154	1756	Rabbit	Use with Caution	1:2000	-20
277	TSC1/Hamartin	TSC1	TSC1	CST	4906	1125	Rabbit	Use with Caution	1:200	-20
278	TSC2/Tuberin (phospho T1462)	Tuberin_pT1462	TSC2	CST	3617	671	Rabbit	Valid	1:30	-20
279	TTF1**	TTF1	NKX2-1	Abcam	ab76013	1081	Rabbit	Valid	1:250	-20
280	Tuberin	Tuberin	TSC2	Abcam	ab32554	670	Rabbit	Valid	1:2500	-20
281	TUFM	TUFM	TUFM	Abcam	ab173300	1842	Rabbit	Valid	1:300	-20
282	Twist	TWIST	TWIST2	Santa Cruz	sc-81417	1353	Mouse	Use with Caution	1:30	4
283	Tyro3	Tyro3	TYRO3	CST	5585	1080	Rabbit	Valid	1:30	-20
284	UBAC1	UBAC1	UBAC1	Sigma-Aldrich	HPA005651	1270	Rabbit	Valid	1:250	-20
285	Ubiquityl Histone H2B	Ubq-Histone-H2B	HIST1H2BB	CST	5546	1662	Rabbit	Use with Caution	1:500	-20
286	UGT1A	UGT1A	UGT1A1	Santa Cruz	sc-271268	1267	Mouse	Valid	1:75	4
287	ULK1 (phospho S757)	ULK1_pS757	ULK1	CST	6888	1626	Rabbit	Use with Caution	1:1000	-20
288	VASP	VASP	VASP	CST	3112	678	Rabbit	Valid	1:250	-20
289	VDAC1/Porin	Porin	VDAC1	Abcam	ab14734	1254	Mouse	Valid	1:300	-20
290	VEGF Receptor 2	VEGFR-2	KDR	CST	2479	688	Rabbit	Valid	1:12000	-20
291	VHL/EPPK1**	VHL-EPPK1	EPPK1	BD Biosciences	556347	693	Mouse	Use with Caution (Targets EPPK1)	1:500	4
292	Vimentin	Vimentin	VIM	Dako	M0725	1393	Mouse	Use with Caution	1:250	4
293	Wee1	Wee1	WEE1	CST	4936	1802	Rabbit	Use with Caution	1:2000	-20
294	Wee1 (Phospho S642)	Wee1_pS642	WEE1	CST	4910	2058	Rabbit	Use with Caution	1:75	-20
295	WIPI1	WIPI1	WIPI1	CST	12124	1627	Rabbit	Use with Caution	1:1000	-20
296	WIPI2	WIPI2	WIPI2	CST	8567	1628	Rabbit	Use with Caution	1:1000	-20
297	XBP1	XBP1	XBP1	Santa Cruz	sc-32136	1044	Goat	Use with Caution	1:200	4
298	XPA	XPA	XPA	Santa Cruz	sc-56813	1359	Mouse	Valid	1:75	4
299	XPF	XPF	XPF	Abcam	ab73720	2015	Rabbit	Use with Caution	1:100	-20
300	XRCC1	XRCC1	XRCC1	CST	2735	906	Rabbit	Use with Caution	1:30	-20
301	YAP	YAP	YAP1	Santa Cruz	sc-15407	780	Rabbit	Use with Caution	1:750	4
302	YAP (phospho S127)	YAP_pS127	YAP1	CST	4911	782	Rabbit	Valid	1:250	-20
303	YB1 (phospho S102)	YB1_pS102	YBX1	CST	2900	835	Rabbit	Valid	1:50	-20
304	ZAP-70	ZAP-70	ZAP70	CST	2705	1828	Rabbit	Use with Caution	1:1000	-20

Additional Antibody Charges

View the Services page for complete pricing information

Antibody Probing
- Investigator-supplied antibody (at least 50 μl) previously validated and probed by core: $300/ab and sample set (+ overhead fee for external out-of-network and non-academic institutions)
Antibody Validation
- Investigator-supplied antibody (at least 100 μl) never validated and probed by core: $750/ab and sample set (+ overhead fee for external out-of-network and non-academic institutions)*
- Please provide at least 120 μl of your positive and negative cell lysates, full length Western blot results and densitometer reading of each band if possible.

*Charge includes validation testing of your antibody as well as probing your RPPA sample set

Antibody Validation for RPPA

Our core extensively validates antibodies applicable to RPPA analysis.

If available, correlation between RPPA and mass spectrometry or RPPA and RNA expression with a Spearman correlation coefficient greater than 0.5. If correlation is less than 0.5, validation will be determined by Western blot.
A single or dominant band on Western blotting is required and dynamic range and specificity is determined using peptides, phosphopeptides, growth factors, inhibitors, RNAi, and/or cells with wide levels of expression including 330 cell lines under multiple conditions on a single array.
A Pearson correlation coefficient between RPPA and Western blotting of greater than 0.7 is required
Reproducible results within a slide and between slides are critical.

Protocols for RPPA Sample Preparation

Cell Pellet Preparation (To submit for protein extraction)

Attached Cells

Collect cells by either trypsin EDTA or scraping cells with rubber policeman (minimum of 1 million cells per sample is required)
Wash cell pellet twice with PBS (resuspend and centrifuge)
Aspirate PBS as much as possible without disturbing cell pellet
Store dry cell pellet in -80°C until sample submission
If shipping samples, pack on plenty of dry ice

Suspension Cells

Collect cells by spinning down by centrifugation (minimum of 1 million cells per sample is required)
Wash cell pellet twice with PBS (resuspend and centrifuge)
Aspirate PBS as much as possible without disturbing cell pellet
Store dry cell pellet in -80°C until sample submission
If shipping samples, pack on plenty of dry ice

Cell Lysate Preparation (From a 6-well plate)

Reagents

Lysis Buffer: 1% Triton X-100, 50mM HEPES pH 7.4, 150mM NaCl, 1.5mM MgCl₂, 1mM EGTA, 100mM NaF, 10mM Na pyrophosphate, 1mM Na₃VO₄, 10% glycerol, freshly added protease and phosphatase inhibitors from Roche Applied Science cat. nos. 05056489001 and 04906837001, respectively. Complete lysis buffer can be stored in -20oC. Before use, thaw on ice.
4xSDS Sample Buffer: 40% Glycerol, 8% SDS, 0.25M Tris-HCL, pH 6.8. Before use, add Beta-mercaptoethanol (B-Me) at 1/10 of the volume.

Procedure

Seed cells in 6-well plates for overnight or 24-hour incubation. Cell number per well is dependent on the cell size, cell growth rate and experimental design (e.g., duration of treatment). Generally, 0.3-0.5x10⁶ cells/3ml is recommended in each well of 6-well plates.
Treat cells according to experimental design.
Wash the cells twice with PBS. Add lysis buffer to the plates (100-150µl for each well of 6-well plate).
Incubate the plates on ice (leveled) for 20 minutes with occasional shaking every 5 minutes.
Scrape cells off the plates and collect the cell lysate into 1.5ml microcentrifuge tubes. Centrifuge the cell lysate in microcentrifuge at 14,000 rpm (maximum speed) for 10 minutes at 4 degrees C.
Carefully collect the supernatant. Discard the pellet.
Determine cellular protein concentration by BCA or Bradford reaction. Adjust protein concentration to 1.5.g/.l. (Use lysis buffer to dilute.)
Mix the cell lysate with (4xSDS + B-Me) sample buffer without bromophenol blue (3 parts cell lysate plus one part 4xSDS sample buffer). Boil the samples for 5 minutes and store in -80 degrees C until sample submission.

Please provide at least 80µl of each sample separately in a 1.5 ml standard flip-cap microcentrifuge tube. Label tubes numerically in order according to your sample list. Do not place stickers on the sides of the tubes, as we will place our own labels there.

Cell Lysate Preparation (From a pellet)

Preparation of Suspension Cell Pellets

Collect cells by spinning down by centrifugation (minimum of 1 million cells per sample is required)
Wash cell pellet twice with PBS (resuspend and centrifuge)
Aspirate PBS as much as possible without disturbing cell pellet

Lysis of Cell Pellets

Reagents
1. Lysis Buffer: 1% Triton X-100, 50mM HEPES pH 7.4, 150mM NaCl, 1.5mM MgCl₂,1mM EGTA, 100mM NaF, 10mM Na pyrophosphate, 1mM Na₃VO₄, 10% glycerol, freshly added protease and phosphatase inhibitors from Roche Applied Science cat. nos. 05056489001 and 04906837001, respectively. Complete lysis buffer can be stored in -20 degrees C. Before use, thaw on ice.
2. 4xSDS Sample Buffer: 40% Glycerol, 8% SDS, 0.25M Tris-HCL, pH 6.8. Before use, add Beta-mercaptoethanol (B-Me) at 1/10 of the volume.
Thaw dry cell pellets on ice. Add 80µl of cold lysis buffer to the pellets and mix by pipetting up and down. Set the sample mixture on ice for 30 minutes, vortexing every 5 to 10 minutes.
1. Note: Volume of lysis buffer to be added depends on the number of cells and the size of the pellet. Minimum of 80µl containing 1-2 million cells per sample is required for sufficient protein concentration for RPPA.
Centrifuge the cell lysate in a microcentrifuge at 14,000 rpm (maximum speed) for 10 minutes at 4 degrees C.
Carefully collect the supernatant. Discard the pellet.
Determine the cellular protein concentration by BCA or Bradford reaction. Adjust protein concentration to 1.5µg/µl. (Use lysis buffer to dilute.)
Mix the cell lysate with 4xSDS sample buffer + B-Me without bromophenol blue (3 parts cell lysate plus one part 4xSDS sample buffer). Boil the samples for 5 minutes and store in -80 degrees C until sample submission.

PBMC Harvesting from EDTA Tubes

Leave samples at room temperature prior to processing on the same day.

Plasma

Place the EDTA tubes containing patient samples into a centrifuge and spin the blood samples at 1,500×g at 4°C for 10 min—the plasma fraction is the top layer and must be handled with care as shaking disturbs the fractions.
Gently transfer the top layer (plasma) from each tube to a 15 ml conical tube. Save the remaining layers in the EDTA tubes.
1. Note: Inspect plasma for turbidity. Turbid samples should be recentrifuged and aspirated again to remove remaining insoluble matter. If hemolysis (pink to red tinge in sample) is observed, this information should be recorded.
Centrifuge at 2,000×g at 4°C for 20 min to remove platelets.
If needed for other assays, transfer the supernatant (plasma without platelets) in aliquots of 1.8 ml into 2.0 ml cryovials (as many as necessary to store all plasma) and immediately store the cryovials at -80°C.

PBMC

After centrifugation and removal of supernatant in Plasma Steps 1 and 2, transfer the middle layer (i.e., buffy coat about 1.0 ml/10 ml blood) from the patient's samples to a new 15 ml conical tube.
Reconstitute the buffy coat by adding at least 2 volumes of PBS (up to 4 ml) and then gently mixing.
Add 4 ml of room-temperature Ficoll-Paque Plus to another 15 ml conical tube.
Very carefully load the diluted PBS/buffy coat mixture onto the top of Ficoll-Paque Plus at room temperature. Make sure that the blood and Ficoll do not mix.
Centrifuge and spin the sample at 1,000×g at room temperature for 20 min with the BRAKE OFF. This procedure will result in four layers
Collect the second cloudy layer (mononuclear cells) and transfer this layer into a new 15 ml conical tube. Add PBS to 10 ml.
Centrifuge and spin the sample at 1,500×g for 10 min at room temperature.
Remove the supernatant and reconstitute the cell pellet by first gently pipetting in 10 ml of PBS and then filling the tube with PBS.
Centrifuge and spin the sample at 1,500×g for 10 min at room temperature.
Repeat steps 8 and 9.
Remove the supernatant then count the cells per the laboratory's standard procedure.
For RPPA analysis, centrifuge at 1,500×g for 10 min at room temperature. Remove as much supernatant as possible. Freeze down the cell pellet immediately in -80°C. Send frozen cell pellets on dry ice to the RPPA Core. 2–5x106 cells per sample are required for RPPA Analysis.

Tissue Lysate Preparation by Precellys homogenizer

Reagents and Materials

Frozen tumor tissue set on dry ice
Scalpel
Weighing dish
Tweezers
Lysis buffer with protease inhibitors set on ice
Tubes from Precellys Ceramic Beads Kit (1.4 mm, Cat # 10011152 from Cayman Chemical)
1.5ml microcentrifuge tubes labeled with sample number and set on ice
Lysis Buffer: 1% Triton X-100, 50mM HEPES pH 7.4, 150mM NaCl, 1.5mM MgCl₂,1mM EGTA, 100mM NaF, 10mM Na pyrophosphate, 1mM Na3VO4, 10% glycerol, containing freshly added protease and phosphatase inhibitors from Roche Applied Science cat. no. 05056489001 and 04906837001, respectively. Completed lysis buffer can be stored in -20 degrees C. Before use, thaw on ice..
4xSDS Sample Buffer: 40% Glycerol, 8% SDS, 0.25M Tris-HCL, pH 6.8. Before use, add Beta-mercaptoethanol (B-Me) at 1/10 of the volume.

Procedure

Remove the tumor tissue from cryovials and set in weighing dish at room temperature for a short while. (Do not wait for complete thaw.) Cut a small piece of tumor tissue (approximately the size of a grain of rice) and place in 2ml tubes with Ceramic Beads (for Precellys homogenizer).
1. We can work with small volumes by Precellys homogenizer. We estimate protein yield at 60µg from 1mg of tissue.
Add ice-cold lysis buffer to the tube. The volume of lysis buffer is calculated as 40mg of tumor/ml.
For using Precellys, place the tubes on the rack, secure the white lid, and choose from program 1 or 2; click valid to start. Program 1 is set at 30 second per cycle for 2 cycles and Program 2 is set at 45 seconds per cycle for 2 cycles.
Centrifuge at 4 degrees C for 15 minutes at maximum speed (13,000-14,000 rpm).
Collect supernatant (tumor lysates) and transfer to another set of microcentrifuge tubes.
Determine the protein concentration by BCA or Bradford reaction and adjust protein concentration to 1.5 ƒÝg/ƒÝl. (Use lysis buffer to dilute)
Mix the cell lysate with 4xSDS + B.-Me sample buffer without bromophenol blue (3 parts cell lysate plus one part 4xSDS sample buffer). Boil the samples for 5 minutes and store in -80 degrees C until sample submission.

Tissue Lysate Preparation by electric/hand homogenizer

Reagents and Materials

Frozen tumor tissue set on dry ice
Scalpel
Weighing dish
Tweezers
Lysis buffer with protease inhibitors set on ice
5ml tubes (round bottom) labeled with sample number and set on ice
1.5ml microcentrifuge tubes labeled with sample number and set on ice
Lysis Buffer: 1% Triton X-100, 50mM HEPES pH 7.4, 150mM NaCl, 1.5mM MgCl₂,1mM EGTA, 100mM NaF, 10mM Na pyrophosphate, 1mM Na3VO4, 10% glycerol, containing freshly added protease and phosphatase inhibitors from Roche Applied Science cat. no. 05056489001 and 04906837001, respectively. Completed lysis buffer can be stored in -20 degrees C. Before use, thaw on ice..
4xSDS Sample Buffer: 40% Glycerol, 8% SDS, 0.25M Tris-HCL, pH 6.8. Before use, add Beta-mercaptoethanol (B-Me) at 1/10 of the volume.

Procedure

Remove the tumor tissue from cryovials and set in weighing dish at room temperature for a short while. (Do not wait for complete thaw.) Cut a small piece of tumor tissue (approximately the size of a grain of rice) and weigh by analytical balance. Try to put the remaining tumor tissue back on dry ice as soon as possible.
Put the small piece of tumor tissue into a 5ml tube on ice. Add ice-cold lysis buffer to the tube. The volume of lysis buffer is calculated as 40mg of tumor/ml.
Homogenize the tumor tissue by electric or hand homogenizer for 8 seconds. The tumor tissue should be set on ice while homogenizing to prevent heat. Wash the homogenizer probe twice with ice-cold water in between samples and dry the probe with Kimwipe.
Optional: Set the samples on ice for 10 minutes.
Transfer the samples to microcentrifuge tubes and centrifuge at 4 degrees C, 14,000rpm for 10 minutes.
Collect supernatant (tumor lysates) and transfer to another set of microcentrifuge tubes.
Determine the protein concentration by BCA or Bradford reaction and adjust protein concentration to 1.5 .g/.l. (Use lysis buffer to dilute)
Mix the cell lysate with 4xSDS + B-Me sample buffer without bromophenol blue (3 parts cell lysate plus one part 4xSDS sample buffer). Boil the samples for 5 minutes and store in -80 degrees C until sample submission.

Materials & Methods

Cellular proteins were denatured in a 1% SDS + 2-mercaptoethanol buffer solution and diluted in five 2-fold serial dilutions in dilution lysis buffer. Serially diluted lysates were arrayed on nitrocellulose-coated slides (Grace Bio-Labs) by the Quanterix (Aushon) 2470 Arrayer (Quanterix Corporation). A total of 5808 spots were arrayed on each slide including spots corresponding to serially diluted (1) standard lysates, and (2) positive and negative controls prepared from mixed cell lysates or dilution buffer, respectively.

Each slide was probed with a validated primary antibody plus a biotin-conjugated secondary antibody. Antibody validation for RPPA is described on the RPPA Core website. Signal detection was amplified using an Agilent GenPoint staining platform (Agilent Technologies) and visualized by DAB colorimetric reaction. The slides were scanned (Huron TissueScope, Huron Digital Pathology) and quantified using customized software (Array-Pro Analyzer, Media Cybernetics) to generate spot intensity.

Relative protein level for each sample was determined by RPPA SPACE (developed by MD Anderson Department of Bioinformatics and Computational Biology, Shehwana et al., 2022) by which each dilution curve was fitted with a logistic model. RPPA SPACE fits a single curve using all the samples (i.e., dilution series) on a slide with the signal intensity as the response variable and the dilution steps as the independent variable. The fitted curve is plotted with the signal intensities, both observed and fitted, on the y-axis and the log2 concentration of proteins on the x-axis for diagnostic purposes. The protein concentrations of each set of slides were then normalized for protein loading. Correction factor was calculated by (1) median-centering across samples of all antibody experiments; and (2) median-centering across antibodies for each sample. Results were then normalized across RPPA sets by replicates-based normalization as described (Akbani et al. 2014).

Details of the RPPA platform as performed by the RPPA Core are described in Siwak et al., 2019.

References

Akbani R, Ng PK, Werner HM, Shahmoradgoli M, Zhang F, Ju Z, Liu W, Yang JY, Yoshihara K, Li J, Ling S, Seviour EG, Ram PT, Minna JD, Diao L, Tong P, Heymach JV, Hill SM, Dondelinger F, Stadler N, Byers LA, Meric-Bernstam F, Weinstein JN, Broom BM, Verhaak RG, Liang H, Mukherjee S, Lu Y, Mills GB. A pan-cancer proteomic perspective on The Cancer Genome Atlas. Nat Commun, 2014. 5: p. 3887. PMID: 24871328 PMCID: 4109726

Shehwana H, Kumar SV, Melott JM, Rohrdanz MA, Wakefield C, Ju Z, Siwak DR, Lu Y, Broom BM, Weinstein JN, Mills GB, Akbani R. RPPA SPACE: an R package for normalization and quantitation of Reverse-Phase Protein Array data. Bioinformatics, 2022 38(22):5131-5133. PMID: 36205581 PMCID: PMC9665860

Siwak DR, Li J, Akbani R, Liang H, Lu Y. Analytical Platforms 3: Processing Samples via the RPPA Pipeline to Generate Large-Scale Data for Clinical Studies. Adv Exp Med Biol, 2019 1188:113-147. PMID: 31820386

Resources

Antibody Lists

Overview

Meaning of Validation Status

Expanded Antibody List: Set 209 to Current

Standard Antibody List: Set 147 to Present

Additional Antibody Charges

Antibody Validation for RPPA

Protocols for RPPA Sample Preparation

Cell Pellet Preparation (To submit for protein extraction)

Attached Cells

Suspension Cells

Cell Lysate Preparation (From a 6-well plate)

Reagents

Procedure

Cell Lysate Preparation (From a pellet)

Preparation of Suspension Cell Pellets

Lysis of Cell Pellets

PBMC Harvesting from EDTA Tubes

Plasma

PBMC

Tissue Lysate Preparation by Precellys homogenizer

Reagents and Materials

Procedure

Tissue Lysate Preparation by electric/hand homogenizer

Reagents and Materials

Procedure

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Materials & Methods

References

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