Submission, Services and Pricing
Submission
Submission Process
- Sign-up for an iLab Solutions account by cutting and pasting the following web address in your brower: https://mdanderson.ilabsolutions.com.
- After your account is set up, request services through the RPPA Core iLab Page.
- When your iLab request is fully approved*, you will receive an email either to schedule sample drop off or shipment and instructions. (*Both the submitter AND PI and/or Financial Manager of the iLab request must log into their own iLab account and "agree" to the request before it is considered fully approved.)
- Label the tops of the sample vials with the sample order (not the sample name) in numerical order according to your sample list in your iLab request. (Do not put labels or tape around the sides of the sample vials as these must be removed before processing.)
- Pack samples on dry ice.
- Drop off or ship samples to the RPPA Core at your previously scheduled drop off/shipment day.
*Please note that our goal is to deliver results within 8-10 weeks after receiving samples. However, based on sample collection, workload, or equipment failure, processing and analysis may occasionally take up to 12 weeks. We do everything possible to shorten the timeline, while maintaining high quality data at a low cost.
Payment
Inside MD Anderson-PeopleSoft Account Number
- Your funding will need to be set up within your iLab account before you can request RPPA services online.
- Your Financial Manager will need to give you access to the correct funding source, so please contact him/her beforehand.
Outside MD Anderson-Purchase Order (PO) or Credit Card
An electronic copy of the PO or Credit Card Charge Authorization Form must be either emailed to ccsgrppa@mdanderson.org or attached to the iLab request before you can submit your samples. The Core will not accept your samples without this.
Services
What We Offer
- Perform reverse phase protein arrays on the samples received from customers
- Perform custom antibody validation using a reverse phase protein array
- Provide standard operating procedures needed for the extraction of proteins from cell lines and tissue for use in the reverse phase protein array studies
- Perform primary data analysis using custom software to determine signal intensity, curve construction and relative protein expression and modification
- Perform protein extraction from tissue or cell pellets, upon request (with additional charge), for use in the reverse phase protein array studies
- Provide updated list of antibodies for reverse phase protein array studies
- Expanded Panel currently comprised of approximately 450 antibodies (for human and mouse cell line samples) and approximately 350 antibodies (for mouse tissue/xenograft samples)
- Provide a turnaround time of 8 to 10 weeks from sample submission. However, based on workload or equipment failure, analysis may take up to 12 weeks.
Data Reports
- Each data report includes your raw, normalized, and median-centered data as well as two heatmaps (one supervised and one un-supervised).
- "Datasets" essentially means how you want your samples grouped and your data split into heatmaps. You might group samples according to experiments, experimental treatments, cell lines, or sample types. For example, if you are submitting human and mouse samples, you might group the human samples in one dataset (dataset 1) and the mouse samples in another (dataset 2).
- If you submit mouse tissue or xenograft samples, mouse and rat antibodies will be excluded from your data due to major issues with the protein loading correction within the RPPA process due to the cross-reactivity of the antibodies with the samples.
- Our RPPA process will not generate heatmaps for only two samples. Therefore, we will not provide heatmaps for submissions that only contain two samples or for datasets that only contain two samples.
Pricing
- Prices are per sample for a panel of up to 500 antibodies (for human and mouse cell line samples) and ≥350 antibodies (for mouse tissue/xenograft samples), AND are subject to change. If an official quote is provided for a grant, we will honor the price quoted (provided the quote is generated within 6 months of sample submission). If the quote is more than 6 months old, or no quote was provided, the new rates will be charged.
- We will provide up to 4 data sets free of charge. Any dataset request over 4 will incur a charge of up to $200/additional data set (+ overhead where applicable). Any data set requested after the original data report is sent will also incur a charge of up to $200/new data set (+ overhead where applicable).
Academic Institutions
MD Anderson Cancer Center (Internal)
RPPA Service: $100/sample
Protein Extraction: $20/sample
4x SDS and/or 2-mercaptoethanol Addition: $10/sample
RPPA Lysis Buffer with/without 4x SDS: $1.50/ml
Additional Antibody Validation (never validated and probed by core): $750/ab
Additional Antibody Probing (previously validated and probed by core): $300/ab
Return Residual Material: $250/sample set
Additional Data Sets: $200/data set
External Academic Institutions (in Network)
External in Network Academic Institutions are listed in the below purple box.
RPPA Service: $155/sample
Protein Extraction: $20/sample
4x SDS and/or 2-mercaptoethanol Addition: $10/sample
RPPA Lysis Buffer with/without 4x SDS: $1.50/ml
Additional Antibody Validation (never validated and probed by core): $750/ab
Additional Antibody Probing (previously validated and probed by core): $300/ab
Return Residual Material: $250/sample set
Additional Data Sets: $200/data set
External Academic Institutions (out of Network)
External out of Network Academic Institutions are subject to a 62% overhead fee.
RPPA Service: $251.10/sample ($155+62% overhead)
Protein Extraction: $32.40/sample ($20+62% overhead)
4x SDS and/or 2-mercaptoethanol Addition: $16.20/sample ($10+62% overhead)
RPPA Lysis Buffer with/without 4x SDS: $2.43/ml ($1.50+62% overhead)
Additional Antibody Validation (never validated and probed by core): $1,215/ab ($750+62% overhead)
Additional Antibody Probing (previously validated and probed by core): $486/ab ($300+62% overhead)
Return Residual Material: $405/sample set ($250+62% overhead)
Additional Data Sets: $324/data set ($200+62% overhead)
Non-Academic Institutions
Non-Academic Institutions are subject to a 69% overhead fee.
RPPA Service: $507/sample ($300+69% overhead)
Protein Extraction: $50.70/sample ($30+69% overhead)
4x SDS and/or 2-mercaptoethanol Addition: $25.35/sample ($15+69% overhead)
RPPA Lysis Buffer with/without 4x SDS: $2.54/ml ($1.50+69% overhead)
Additional Antibody Validation (never validated and probed by core): $1,267.50/ab ($750+69% overhead)
Additional Antibody Probing (previously validated and probed by core): $507/sample ($300+69% overhead)
Return Residual Material: $422.50/sample set ($250+69% overhead)
Additional Data Sets: $338/data set ($200+69% overhead)
External in Network Academic Institutions
- UT Systems
- Baylor College of Medicine (BCM)
- Houston Methodist Research Institute (HMRI)
- Oregon Health & Science University (OHSU)
- Rice University
- Texas A&M Institute of Biosciences & Technology (IBT)
- University of Houston (UH)