Flow Cytometry and Cellular Imaging Core - Smithville
Ellen Richie, Ph.D. and Collene Jeter, Ph.D., Co-Directors
The Flow Cytometry and Cell Imaging Core facility in the Department of Epigenetics and Molecular Carcinogenesis at Science Park provides cutting-edge resources to dissect the cellular and molecular mechanisms of developmental and disease processes. This CPRIT-funded (RP 170628) core facility is home to the complementary resources of flow cytometry, confocal, and two-photon microscopy. The personnel and equipment in these cores aid researchers as they identify, isolate and quantify cancer and normal cell subsets as well as image cells and their constituent molecules in situ. Applications include: cellular and molecular phenotyping, analysis of subcellular and nuclear architecture, identification of drug-genome interactions, 3D reconstructions and time-lapse imaging to discern spatiotemporal molecular and cellular relationships. The FCCIC also offers individualized training on core instruments and assistance with data analysis and experimental design.
Use of this core requires acknowledgement of CPRIT Core Facility Support Grant (RP170628) in your publications regardless of funding source.
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Flow Cytometry
Flow cytometry provides investigators with the ability to isolate rare, viable cell subsets from different types of normal and tumor single-cell suspensions based on differential expression of surface antigens. Multiparameter immunofluorescence analysis also allows investigators to quantify the frequency of cell subsets that express various combinations of surface and/or cytoplasmic proteins in the same cell preparation. Additional analytical services include analysis of apoptosis, cell cycle analysis, single-cell deposition and identification of side-population stem cells. The core also offers individualized training on BD FACSAria Fusion cell sorters and the BD LSRFortessa™ for unassisted operation, and assistance with data analysis and experimental design.
See our main Flow Cytometry page to learn more about our:
Cellular Imaging
Cellular imaging provides investigators with the ability to capture high resolution images of cells and their constituent molecules in a physiologically relevant context in situ. The extreme sensitivity and high temporal resolution of our Zeiss LSM880 laser scanning confocal microscope with FAST Airyscan super-resolution module allows investigators to acquire high contrast multispectral optical sections of fixed and living cells, tissues and tumor specimens. Multiphoton microscopy with our cutting-edge Leica TCS SP8 DIVE system permits the study of development and tumor pathophysiology by deep imaging of intact (or vibratome cut) thick tissues or animals and is ideal for studies such as analysis of tissue and tumor architecture, angiogenesis and cancer-stromal cell interactions Additional microscopy applications include microirradiation to study DNA-damage repair, z-stacking and 3D reconstructions, protein-protein interactions (e.g., FRET) and time-lapse imaging of dynamic cell and molecular processes.
See our main Cellular Imaging page to learn more about our:
Contact Us
Facility Directors and Staff
Ellen Richie, Ph.D.
Co-Director, FCCIC
Professor
Epigenetics and Molecular Carcinogenesis
Phone: 512-237-9435
Email: erichie@mdanderson.org
Collene Jeter, Ph.D.
Co-Director FCCIC
Confocal Manager
Assistant Professor
Epigenetics and Molecular Carcinogenesis
Phone: 512-237-6491
Email: cjeter@mdanderson.org
Pamela Whitney
Lab Manager, Flow Cytometry
Expertise: Cytofluorimetric analysis and cell sorting
Lab: 512-237-9427
Office: 512-237-6522
Email: pjwhitney@mdanderson.org
Jennifer Terpstra
Research Assistant II
Expertise: Vibratome sectioning, tissue processing and immunofluorescence staining
Phone: 512-237-6443
Email: jjterpstra@mdanderson.org
Facility Location
The Virginia Harris Cockrell Cancer Research Center at the University of Texas MD Anderson Cancer Center, Science Park Department of Epigenetics and Molecular Carcinogenesis
Mailing Address: P.O. Box 389, Smithville, Texas 78957
Physical and Shipping Address: 1808 Park Road 1C, Smithville, Texas 78957