Epigenomics Profiling Core
- Research Resources
- Core Facilities and Services
- Epigenomics Profiling Core
Overview
The Epigenomics Profiling Core (EpiCore) is an institutional resource for epigenomics research that brings scientific expertise, advanced assays and specialized equipment not readily available to MD Anderson Cancer Center researchers. We offer cost-effective, full-service protocols to investigate DNA methylation (Pyrosequencing Methylation Analysis, RRBS, cfMeDIP-seq, WGBS), chromatin accessibility (ATAC-Seq) and DNA-protein interactions (ChIP-Seq and CUT&RUN). Our overall goal is to facilitate researchers’ efforts to understand epigenetic mechanisms that alter the chromatin state that regulate the transcription output in normal and disease conditions. Learn more about the Epigenomics Profiling Core.
Getting Started
Each project starts with a consultation with core directors to design experimental option(s). Please contact Marcos Estecio, Ph.D., (mestecio@mdanderson.org) for DNA methylation analysis services and Abhinav Jain, Ph.D., (ajain@mdanderson.org) for chromatin analysis services (ChIP-seq, CUT&RUN and ATAC-seq).
For additional scheduling, please use iLab.
Impact
We offer a one-stop unique resource for expertise, methods and services to assess the epigenome for basic and clinical applications, such as determining effectiveness of epigenetic therapies. The core directors work closely with the investigators to adapt protocols to match the needs of specific biological samples, data interpretation, manuscript and grant preparation.
Contact
DNA Methylation Analysis Services Marcos R. Estecio, Ph.D. |
Chromatin Analysis Services Abhinav Jain, Ph.D. |
Location:
The University of Texas MD Anderson Cancer Center
Basic Sciences Research Building - S9.8414
6767 Bertner Ave.
Houston, TX 77030
New Services:
We are now offering the following services:
- Bioinformatics Analysis of ChIP-Seq, ATAC-Seq and RRBS libraries
- cfMeDIP (Circulating Cell-free Methylated DNA Immunoprecipitation)
- CUT&RUN (Cleavage Under Targets and Release Using Nuclease)
Coming Soon:
cfChIP-Seq of cell-free DNA from liquid biopsies
Chromatin Immunoprecipitation and sequencing of cell-free nucleosomes from human plasma (cfChIP-seq) to sequence DNA fragments from nucleosomes carrying specific chromatin marks
Direct DNA and RNA modifications profiling by Nanopore
Sequencing The EpiCore has recently acquired a GridION MK1 Sequencing Instrument (Oxford Nanopore Technologies) and will soon offer direct detection of base modifications in DNA and RNA.
Acknowledgment
Publications that utilize data generated through EpiCore should cite the Epigenomics Profiling Core in the Acknowledgment section. Please send us a copy of the accepted or published manuscript with journal name and date of acceptance.