The Proteomics and Metabolomics Facility provides state-of-the-art mass spectrometry analysis of proteins and metabolites for both basic and clinical cancer research. We strive to serve all internal and external investigators by providing access to mass-spectrometry based proteomics and metabolomics technologies and services.
What is Proteomics?
- Proteomics is the study of proteomes or the systematic analysis of proteins.
- As defined by Merriam-Webster dictionary: "a branch of biotechnology concerned with applying the techniques of molecular biology, biochemistry and genetics to analyze the structure, function and interactions of the proteins produced by the genes of a particular cell, tissue or organism, with organizing the information in databases, and with applications of the data (as in medicine or biology)".
- Proteomics includes not only quantitative and qualitative analysis of all expressed proteins, but also traditional protein structural studies such as protein identification, post-translational modification, protein-protein interactions, and other functional interests related with protein structure.
- Common sample types include gels, solutions, immunoprecipitates, and other protein-containing materials.
What is Metabolomics?
- Metabolomics is the study of small molecules (defined generally by molecular weight less than 1500 Daltons) in biological samples, including both relative and absolute quantitation.
- “Targeted metabolomics” refers to the analysis of a defined subset of molecules, whereas “non-targeted metabolomics” refers to the analysis of all detectable small molecules.
- In addition to measuring total metabolite levels, a second workflow involves isotope tracer analysis (in either targeted or non-targeted mode), in which stable isotope-labeled metabolites can be used to track the flow of atoms in metabolism.
- The most common biological matrices in which we measure metabolites are: cells, media, plasma, whole blood, red blood cells, urine, and tissues. But even more complex matrices (e.g., bone) can be analyzed.
How Can We Help You?
Please visit our Services and Pricing page for more information.
This facility is funded by NIH High-End Instrumentation program grant# 1S10OD012304-01, and CPRIT Core Facility Grant# RP130397. Please acknowledge our Facility and these grants in your citations.