ORION Core Facility
Cara Haymaker, Ph.D.
Director
What is ORION?
The ORION (Oncology Research and Immuno-mONitoring) core provides state-of-the-art immunoprofiling services including clinical trial immune monitoring, data analysis and technical consultation for assay design, as well as single service instrument use.
Available assays include soluble factor assessment (Luminex 200, MSD QuickPlex SQ120), detection of antigen-specific T cells (CTL ELISPOT analyzer) and 18 parameter, high-order flow cytometry of tumor tissue and/or PBMCs (BD Fortessa X20).We provide training on CTL ELISPOT analyzer and MSD QuickPlex SQ120 for non-assisted use so investigators may use them independently.
The ORION core also offers cost-effective and customized research support to MD Anderson and local investigators for experimental design and assay development.
Core Grant Citation
The CCSG grant supports the ORION core (NIH P30CA016672). Please
include the following acknowledgement in research publications
supported by the core: "Supported by the NIH/NCI under award
number P30 CA016672 and used the ORION core".
Instrumentation
- Luminex 200 (Full service and Instrument use)
- MSD QuickPlex SQ120 (Full service and Instrument use)
- CTL ELISPOT analyzer (Full service and Instrument use)
- BD Fortessa X-20; High-order flow cytometry: up to 18 colors (Full service)
- BD FACS Jazz (Full service)
ORION core offers a variety of services including full service and instrument use for single assay
Assay request is available via iLAB, 24 hour per day.
Sample deposit is available via ITB pick up by ORION core, personal delivery and free delivery by MD Anderson courier.
All data (Raw and basic analysis) is available via private, personal BOX service, for free.
Resources
Services request: iLab
Courier Services: (MD Anderson employees) Biological specimens delivered within 1 hour of pick-up. Hours of operation are Monday through Friday, 7 a.m- 4 p.m.
- Phone: 713-792-7491
- Email: Courier@mdanderson.org
Luminex:
- Invitrogen ProcartaPlex Immunoassays
- ProcartaPlex Analyst Software (free download, Windows or Mac)
- Bio-Rad Bio-Plex Assay Builder
- Millipore Milliplex Analyte Finder or Design Your Own Kit
MSD:
- Meso Scale Discovery
- Assay/Analyte List
- Custom Assay Designer
- Discovery Workbench Analysis Software (free download, Windows only)
ELISPOT: