FCCIF South Services

Analytical Flow Cytometry

Flow Cytometry is an important tool in all aspects of cell biology research. Many different combinations of antibody-based stains, fluorescent protein expression and viability indicators are measured at the single cell level. The core provides access to cytometry equipment and staff with expertise to assist researchers in developing assays.

BD FACSCelesta

• 405nm, 488nm and 561nm lasers, 12-color
• Sample acquisition: 5ml FACS tubes, 96 or 384 well plates

Beckman Coulter Gallios

• 405nm, 488nm, and 633nm lasers, 10-color
• Sample acquisition: 32 tube carousel loader

High Parameter Analytical Flow Cytometry

Flow cytometry instrumentation in the core can detect up to 18+ fluorescent parameters at once on the single cell level. The core staff assists researchers in developing high parameter assays, including panel design, instrument set up, assay troubleshooting and data acquisition. Users with complex assays are encouraged to contact core personnel during the planning stages of their experiments. 

Cytek Aurora

• 5-lasers 16UV-16V-14B-10YG-8R, 64 fluorescence channels, Spectral and conventional analysis
• Sample acquisition: 5ml FACS tubes, 96 or 384 well plates

Beckman Coulter Cytoflex LX

• 5-lasers 375nm-60mW, 405nm-80mW, 488nm-50mW, 561nm-30mW and 638nm-50mW lasers, 19-color
• Sample acquisition: 5ml FACS tubes, 96, 96 deep well and 384 well plates

Sony ID7000

• 6-lasers 320nm, 355nm, 405nm, 488nm, 561nm, 637nm, 182 detectors
• Sample acquisition: 24 tube rack 5ml, 96 well plate (standard), 96 well plate (deep), 96 well plate (half deep), 384 well plate (standard)

BD FACSymphony A5SE

• 5-lasers 10UV-14V-9B-9YG-6R, Spectral and conventional analysis
• Sample acquisition: 5ml FACS tubes, 96 or 384 well plates

BD LSRFortessa X-20

• 5-lasers 355nm, 405nm, 488nm, 561nm and 640nm lasers, 18-color
• Sample acquisition: 5ml FACS tubes, 96 or 384 well plates

Cell Sorting

The core uses electrostatic droplet-based high-speed cell sorting to isolate a wide variety of cells based on many different combinations of fluorescence markers. Several nozzle size/pressure configurations are available. Up to 21 color cell sorting is provided with a variety of options for collection vessels, including various tube sizes and multi-well plates. Cell sorting can entail isolation of single cells as well as tube-based collection of up to six simultaneous populations for RNASeq, culture or many other downstream applications.

BD FACSAria III

• 4-lasers 405nm, 488nm, 633nm, 16-color

BD FACSAria Fusion

• 6-lasers 407nm, 488nm, 633nm, 15-color

BD FACS Symphony S6

• 5-lasers 355nm, 405nm, 488nm, 561nm, 633nm

Cytek Aurora Cell Sorter

• 5-lasers 16UV-16V-14B-10YG-8R, 64 fluorescence channels