The Cytometry and Cellular Imaging Core Facility is directed and managed by research professionals who provide users with the ability to
obtain high-quality data and sorted cell populations derived from complex instrumentation that requires specialized training to use and
maintain. In addition to operating, maintaining and analyzing data from flow cytometers, cell sorters and confocal microscopes, staff members
provide protocols and training and consult with users on a one-on-one basis regarding new assays.
Andreeff, M.D., Ph.D.
Andreeff joined MD Anderson in 1990 after having worked at the Memorial Sloan-Kettering Cancer Center (MSKCC) in New York, NY, in the Departments of Pathology and Leukemia. Andreeff has been a pioneer in flow cytometry since 1971, when he established the first flow cytometry laboratory at the University of Heidelberg and organized the first European meeting on flow cytometry. In 1977, he joined Drs. Melamed and Darzynkiewicz at MSKCC and became head of the Leukemia Cell Biology and Hematopathology flow cytometry laboratory. He is professor of medicine and holds the Paul and Mary Haas Chair in Genetics. He is PI of a P01 grant entitled “The Therapy of AML” and participates as PI in the Leukemia and Breast Cancer SPOREs and the CML P01 at MD Anderson. He heads the Section of Molecular Hematology and Therapy, which is adjacent to the North Campus Flow Cytometry Core Facility. Andreeff provides overall direction of the Core facility and assists users with the design and implementation of their experiments. He develops and evaluates new technologies and applications. In the last five years, he recruited and trained Douglas Weidner, Ph.D., Randall Evans, Ph.D., and Jared Burks, Ph.D., as well as Wendy Schober, and selected and secured funds from the institution for new equipment. He will continue to dedicate 10% of his time to the Core facility.
Jared Burks, Ph.D.
Burks obtained his Ph.D. from Texas A&M University working on protein trafficking to the cell surface and nucleus during baculovirus infection. His post-doctoral work at Baylor College of Medicine in the department of Surgery focused on p53 protein trafficking during CMV infection. He has more than 13 years experience in microscopic imaging techniques, in particular spinning disc confocal microscopy. Burks was recruited into the Core laboratory in 2007 where he has developed his background in flow cytometry. He is in charge of cellular imaging on the North Campus and directs operations of the North Campus facility.
Karen Clise-Dwyer, Ph.D.
Clise-Dwyer is Associate Professor in the Department of Stem Cell Transplantion, Transplant Immunology Section. She has more than 20 years experience in cytometry. She earned her PhD in biochemistry at the University of Wisconsin-Madison, where she studied the effects of a BAFF-R mutation on development of humoral immunity. While in graduate school, she developed her interest in flow cytometry and cell sorting instrumentation. She acquired expertise in cell sorting using multi-laser BD FACS Vantage and Digital Vantage (DiVa) cell sorters. When the UW-Madison Core Facility beta-tested the DiVa digital upgrade system, Clise-Dwyer was heavily involved in that effort and she continues to be active in field testing of new instrumentation. She did postdoctoral research at the Trudeau Institute, studying the effects of aging on lymphocyte development before joining MD Anderson. In 2008, Clise-Dwyer was recruited to direct operations at the South Campus Flow Cytometry & Cell Sorting Core Laboratory. She is a member of the International Society for the Advancement of Cytometry (ISAC) and is a former President of the regional cytometry user's group, FlowTex, a major source for cytometry education in the Texas Medical Center and beyond. Clise-Dwyer is always available to meet with core lab users and PI’s upon request; she collaborates with and provides advice to many researchers throughout the MD Anderson community.