The MD Anderson Genetically Engineered Mouse Facility is fully equipped for the efficient production of transgenic and chimeric mice. The facility is comprised of rooms dedicated for the housing of mice, tissue culture use, microinjection and embryo manipulations, DNA preparation, cryopreservation and materials/embryo storage. The microinjection room is equipped with Leica and Zeiss microinjection scopes and Zeiss dissecting scopes. There are two biological safety cabinets along with a double stacked tissue culture incubator located separately in a room dedicated for tissue culture. There is also a liquid nitrogen storage system for the preservation of ES cells and cryopreservation of mouse embryos and germplasm. All mice are housed under Specific Pathogen Free conditions in three rooms capable of holding over 2,500 mice. Batches of archived (cryopreserved) samples are split for storage in Houston and offsite.
MD Anderson Cancer Center, Mitchell Basic Sciences Research Building (BSRB)
Access through Floor 2, J Elevator
Jan Parker-Thornburg, Ph.D.
Co-Director of the GEMF
Primary contact person
Pronuclear injections, embryo cryopreservation, rederivation
Zhezong (Charlie) Luo
ES cell culture, sperm cryopreservation
Blastocyst injections, MRF mouse resources, sperm cryopreservation, IVF
Blastocyst injections, sperm cryopreservation, embryo cryopreservation, IVF, rederivation
Animal care and breeding
VetMed and Surgery, BSRB, Floor 2