The MD Anderson Genetically Engineered Mouse Facility is fully equipped for the efficient production of genetically modified mice. The facility is comprised of rooms dedicated for the housing of mice, tissue culture use, microinjection and embryo manipulations, DNA preparation, cryopreservation and materials/embryo storage. The microinjection room is equipped with Leica and Zeiss microinjection scopes and Zeiss dissecting scopes. There are two biological safety cabinets along with a double stacked tissue culture incubator located separately in a room dedicated for tissue culture. There is also a liquid nitrogen storage system for the preservation of ES cells and cryopreservation of mouse embryos and sperm. All mice are housed under Specific Pathogen Free conditions in one room capable of holding over 2,500 mice. Batches of archived (cryopreserved) samples are split for storage in Houston and offsite.
MD Anderson Cancer Center, Mitchell Basic Sciences Research Building (BSRB)
Access through Floor 2, J Elevator
Fernando Benavides, DVM, PhD, DACLAM
Professor and Director of Laboratory Animal Genetic Services
Department of Epigenetics and Molecular Carcinogenesis
Carlos J. Perez, DVM, Ph.D.
Senior Research Scientist
CRISPR/Cas9 Gene Targeting Design
Blastocyst injections, MRF mouse resources, sperm cryopreservation, embryo cryopreservation, IVF
CRISPR/Pronuclear injections, blastocyst injections, sperm cryopreservation, embryo cryopreservation, IVF, rederivation
Animal care and breeding
VetMed and Surgery, BSRB, Floor 2