It is critical that you contact the director of GEMF at firstname.lastname@example.org to discuss your project before submitting a request. We may not accept reagents directly from principal investigators and therefore highly advise to get in touch before ordering any reagent.
To obtain any service from the GEMF
- Contact Fabien Delerue (email@example.com or 713- 563-5827) prior to starting any project.
- Read the below Policies and Procedures section for important information regarding offered by the GEMF.
- Log in to https://mdanderson.ilabsolutions.com, register for an account, then go to the site to request the service(s).
Recombinant DNA Experiments
Because recombinant DNA and gene editing technologies induce genomic modifications, the principal investigators must have approval to use the GEMF deliverables from their Institutional Biosafety Committee (IBC) prior to beginning the experiments.
Prior to starting any services, investigators are also required to obtain approval from their Institutional Animal Care and Use Committee (IACUC). The GEMF cannot initiate any animal experiments without a valid animal use protocol number.
All information related to the experiment will be held fully confidential by the GEMF. No knowledge related to the experiment will be shared unless otherwise approved by the principal investigator.
Submission of a Project Request Form is required prior to the initiation of service by the GEMF. This form will be submitted for billing at the time service is performed, regardless of the final outcome. Please note that procedures are not guaranteed. For all experiments, agistments costs past the weaning date will be charged to the principal investigator.
The MD Anderson Genetically Engineered Mouse Facility is supported in part by a Cancer Center Support Grant (CCSG) from the National Cancer Institute. Therefore, all publications resulting from the services provided by the GEMF must cite the facility in the acknowledgements section. A copy of all publications resulting from animals created by the GEMF should be submitted to Fabien Delerue (Department of Genetics).
Service will be scheduled upon the receipt of all pertinent forms and documentation.
Specific Policies for Experiments
The investigator is responsible for demonstrating a reliable scheme for identifying detection of the transgene at a single copy level by providing results from pilot experiments for this purpose. An aliquot of the plasmid needs to be provided to the GEMF for purification. The GEMF will provide protocols for single-copy detection upon request.
Gene Targeting in zygotes
Before initiating or designing any project, please contact Fabien Delerue. The GEMF is not liable for any project that have been designed and/or implemented without contacting the director.
As part of our Quality Control (QC), the GEMF will thaw one straw of frozen embryos and culture overnight to the blastocyst stage to ensure that embryo freezing is feasible for a particular mouse line.
For sperm cryopreservation, the investigator will be given a schedule for pre-mating the males to encourage new sperm production. Frozen sperm from each male will be tested by IVF for its fertilization capacity. A project is considered complete if the fertilization rate equals or exceeds 20% for each male.