Publications

2025

Hu J, Singh H, Jin Y, Zhang W, Wang J, Xia X, Somaiah N, Gorlick R, Li S. Collagen-disrupting attIL12 TIL therapy boosts deep T cell infiltration via dual signaling activation and CCKAR reduction in sarcomas. Proc Natl Acad Sci U S A. 2025 Oct 14;122(41):e2507542122. doi: 10.1073/pnas.2507542122. Epub 2025 Oct 6. PubMed PMID: 41052334; PubMed Central PMCID: PMC12541433.

2024

Rezvan A, Romain G, Fathi M, Heeke D, Martinez-Paniagua M, An X, Bandey IN, Montalvo MJ, Adolacion JRT, Saeedi A, Sadeghi F, Fousek K, Puebla-Osorio N, Cooper LJN, Bernatchez C, Singh H, Ahmed N, Mattie M, Bot A, Neelapu S, Varadarajan N. Identification of a clinically efficacious CAR T cell subset in diffuse large B cell lymphoma by dynamic multidimensional single-cell profiling. Nat Cancer. 2024 Jul;5(7):1010-1023. doi: 10.1038/s43018-024-00768-3. Epub 2024 May 15. PubMed PMID: 38750245; PubMed Central PMCID: PMC12345446.

2022

Singh H, Srour SA, Milton DR, McCarty J, Dai C, Gaballa MR, Ammari M, Olivares S, Huls H, De Groot E, Marin D, Petropoulos D, Olson AL, Anderlini P, Im JS, Khouri I, Hosing CM, Rezvani K, Champlin RE, Shpall EJ, Cooper LJN, Kebriaei P. Sleeping beauty generated CD19 CAR T-Cell therapy for advanced B-Cell hematological malignancies. Front Immunol. 2022;13:1032397. doi: 10.3389/fimmu.2022.1032397. eCollection 2022. PubMed PMID: 36439104; PubMed Central PMCID: PMC9684710.

Romain G, Strati P, Rezvan A, Fathi M, Bandey IN, Adolacion JRT, Heeke D, Liadi I, Marques-Piubelli ML, Solis LM, Mahendra A, Vega F, Cooper LJ, Singh H, Mattie M, Bot A, Neelapu SS, Varadarajan N. Multidimensional single-cell analysis identifies a role for CD2-CD58 interactions in clinical antitumor T cell responses. J Clin Invest. 2022 Sep 1;132(17). doi: 10.1172/JCI159402. PubMed PMID: 35881486; PubMed Central PMCID: PMC9433104.

2021

Tambaro FP, Singh H, Jones E, Rytting M, Mahadeo KM, Thompson P, Daver N, DiNardo C, Kadia T, Garcia-Manero G, Chan T, Shah RR, Wierda WG. Autologous CD33-CAR-T cells for treatment of relapsed/refractory acute myelogenous leukemia. Leukemia. 2021 Nov;35(11):3282-3286. doi: 10.1038/s41375-021-01232-2. Epub 2021 Apr 8. PubMed PMID: 33833386; PubMed Central PMCID: PMC8550958.

Bandey IN, Adolacion JRT, Romain G, Paniagua MM, An X, Saeedi A, Liadi I, You Z, Rajanayake RB, Hwu P, Singh H, Cooper LJ, Varadarajan N. Designed improvement to T-cell immunotherapy by multidimensional single cell profiling. J Immunother Cancer. 2021 Mar;9(3). doi: 10.1136/jitc-2020-001877. PubMed PMID: 33722906; PubMed Central PMCID: PMC7970283.

2020

Greenbaum U, Yalniz FF, Srour SA, Rezvani K, Singh H, Olson A, Blumenschein G Jr, Hong DS, Shpall EJ, Kebriaei P. Chimeric Antigen Receptor Therapy: How Are We Driving in Solid Tumors? Biol Blood Marrow Transplant. 2020 Oct;26(10):1759-1769. doi: 10.1016/j.bbmt.2020.06.020. Epub 2020 Jul 2. Review. PubMed PMID: 32623078; PubMed Central PMCID: PMC11409837.

Srour SA, Singh H, McCarty J, de Groot E, Huls H, Rondon G, Qazilbash M, Ciurea S, Bardelli G, Buck J, Alousi A, Nieto Y, Rezvani K, Marin D, Popat U, Hosing C, Shpall EJ, Wierda WG, Kantarjian H, Champlin RE, Cooper LJ, Kebriaei P. Long-term outcomes of Sleeping Beauty-generated CD19-specific CAR T-cell therapy for relapsed-refractory B-cell lymphomas. Blood. 2020 Mar 12;135(11):862-865. doi: 10.1182/blood.2019002920. PubMed PMID: 31961918; PubMed Central PMCID: PMC8212349.

2019

Lu H, Li J, Martinez-Paniagua MA, Bandey IN, Amritkar A, Singh H, Mayerich D, Varadarajan N, Roysam B. TIMING 2.0: high-throughput single-cell profiling of dynamic cell-cell interactions by time-lapse imaging microscopy in nanowell grids. Bioinformatics. 2019 Feb 15;35(4):706-708. doi: 10.1093/bioinformatics/bty676. PubMed PMID: 30084956; PubMed Central PMCID: PMC6378937.

Liadi I, Singh H, Romain G, Roysam B, Cooper LJ, Varadarajan N. Defining potency of CAR⁺ T cells: Fast and furious or slow and steady. Oncoimmunology. 2019;8(10):e1051298. doi: 10.1080/2162402X.2015.1051298. eCollection 2019. PubMed PMID: 31646063; PubMed Central PMCID: PMC6791448.

2018

Crossland DL, Denning WL, Ang S, Olivares S, Mi T, Switzer K, Singh H, Huls H, Gold KS, Glisson BS, Cooper LJ, Heymach JV. Antitumor activity of CD56-chimeric antigen receptor T cells in neuroblastoma and SCLC models. Oncogene. 2018 Jul;37(27):3686-3697. doi: 10.1038/s41388-018-0187-2. Epub 2018 Apr 6. PubMed PMID: 29622795.

2017

Kebriaei P, Izsvák Z, Narayanavari SA, Singh H, Ivics Z. Gene Therapy with the Sleeping Beauty Transposon System. Trends Genet. 2017 Nov;33(11):852-870. doi: 10.1016/j.tig.2017.08.008. Epub 2017 Sep 27. Review. PubMed PMID: 28964527.

2016

Hurton LV, Singh H, Najjar AM, Switzer KC, Mi T, Maiti S, Olivares S, Rabinovich B, Huls H, Forget MA, Datar V, Kebriaei P, Lee DA, Champlin RE, Cooper LJ. Tethered IL-15 augments antitumor activity and promotes a stem-cell memory subset in tumor-specific T cells. Proc Natl Acad Sci U S A. 2016 Nov 29;113(48):E7788-E7797. doi: 10.1073/pnas.1610544113. Epub 2016 Nov 14. PubMed PMID: 27849617; PubMed Central PMCID: PMC5137758.

Thokala R, Olivares S, Mi T, Maiti S, Deniger D, Huls H, Torikai H, Singh H, Champlin RE, Laskowski T, McNamara G, Cooper LJ. Redirecting Specificity of T cells Using the Sleeping Beauty System to Express Chimeric Antigen Receptors by Mix-and-Matching of VL and VH Domains Targeting CD123+ Tumors. PLoS One. 2016;11(8):e0159477. doi: 10.1371/journal.pone.0159477. eCollection 2016. PubMed PMID: 27548616; PubMed Central PMCID: PMC4993583.

Kebriaei P, Singh H, Huls MH, Figliola MJ, Bassett R, Olivares S, Jena B, Dawson MJ, Kumaresan PR, Su S, Maiti S, Dai J, Moriarity B, Forget MA, Senyukov V, Orozco A, Liu T, McCarty J, Jackson RN, Moyes JS, Rondon G, Qazilbash M, Ciurea S, Alousi A, Nieto Y, Rezvani K, Marin D, Popat U, Hosing C, Shpall EJ, Kantarjian H, Keating M, Wierda W, Do KA, Largaespada DA, Lee DA, Hackett PB, Champlin RE, Cooper LJ. Phase I trials using Sleeping Beauty to generate CD19-specific CAR T cells. J Clin Invest. 2016 Sep 1;126(9):3363-76. doi: 10.1172/JCI86721. Epub 2016 Aug 2. PubMed PMID: 27482888; PubMed Central PMCID: PMC5004935.

Caruso HG, Torikai H, Zhang L, Maiti S, Dai J, Do KA, Singh H, Huls H, Lee DA, Champlin RE, Heimberger AB, Cooper LJ. Redirecting T-Cell Specificity to EGFR Using mRNA to Self-limit Expression of Chimeric Antigen Receptor. J Immunother. 2016 Jun;39(5):205-17. doi: 10.1097/CJI.0000000000000126. PubMed PMID: 27163741; PubMed Central PMCID: PMC5847343.

2015

Caruso HG, Hurton LV, Najjar A, Rushworth D, Ang S, Olivares S, Mi T, Switzer K, Singh H, Huls H, Lee DA, Heimberger AB, Champlin RE, Cooper LJ. Tuning Sensitivity of CAR to EGFR Density Limits Recognition of Normal Tissue While Maintaining Potent Antitumor Activity. Cancer Res. 2015 Sep 1;75(17):3505-18. doi: 10.1158/0008-5472.CAN-15-0139. PubMed PMID: 26330164; PubMed Central PMCID: PMC4624228.

Merouane A, Rey-Villamizar N, Lu Y, Liadi I, Romain G, Lu J, Singh H, Cooper LJ, Varadarajan N, Roysam B. Automated profiling of individual cell-cell interactions from high-throughput time-lapse imaging microscopy in nanowell grids (TIMING). Bioinformatics. 2015 Oct 1;31(19):3189-97. doi: 10.1093/bioinformatics/btv355. Epub 2015 Jun 9. PubMed PMID: 26059718; PubMed Central PMCID: PMC4693004.

Deniger DC, Yu J, Huls MH, Figliola MJ, Mi T, Maiti SN, Widhopf GF 2nd, Hurton LV, Thokala R, Singh H, Olivares S, Champlin RE, Wierda WG, Kipps TJ, Cooper LJ. Sleeping Beauty Transposition of Chimeric Antigen Receptors Targeting Receptor Tyrosine Kinase-Like Orphan Receptor-1 (ROR1) into Diverse Memory T-Cell Populations. PLoS One. 2015;10(6):e0128151. doi: 10.1371/journal.pone.0128151. eCollection 2015. PubMed PMID: 26030772; PubMed Central PMCID: PMC4451012.

Krishnamurthy J, Rabinovich BA, Mi T, Switzer KC, Olivares S, Maiti SN, Plummer JB, Singh H, Kumaresan PR, Huls HM, Wang-Johanning F, Cooper LJ. Genetic Engineering of T Cells to Target HERV-K, an Ancient Retrovirus on Melanoma. Clin Cancer Res. 2015 Jul 15;21(14):3241-51. doi: 10.1158/1078-0432.CCR-14-3197. Epub 2015 Mar 31. PubMed PMID: 25829402; PubMed Central PMCID: PMC4506228.

Liadi I, Singh H, Romain G, Rey-Villamizar N, Merouane A, Adolacion JR, Kebriaei P, Huls H, Qiu P, Roysam B, Cooper LJ, Varadarajan N. Individual Motile CD4(+) T Cells Can Participate in Efficient Multikilling through Conjugation to Multiple Tumor Cells. Cancer Immunol Res. 2015 May;3(5):473-82. doi: 10.1158/2326-6066.CIR-14-0195. Epub 2015 Feb 24. PubMed PMID: 25711538; PubMed Central PMCID: PMC4421910.

Singh H, Moyes JS, Huls MH, Cooper LJ. Manufacture of T cells using the Sleeping Beauty system to enforce expression of a CD19-specific chimeric antigen receptor. Cancer Gene Ther. 2015 Mar;22(2):95-100. doi: 10.1038/cgt.2014.69. Epub 2015 Jan 16. Review. PubMed PMID: 25591810.

2014

Kumaresan PR, Manuri PR, Albert ND, Maiti S, Singh H, Mi T, Roszik J, Rabinovich B, Olivares S, Krishnamurthy J, Zhang L, Najjar AM, Huls MH, Lee DA, Champlin RE, Kontoyiannis DP, Cooper LJ. Bioengineering T cells to target carbohydrate to treat opportunistic fungal infection. Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):10660-5. doi: 10.1073/pnas.1312789111. Epub 2014 Jul 7. PubMed PMID: 25002471; PubMed Central PMCID: PMC4115509.

Singh H, Huls H, Kebriaei P, Cooper LJ. A new approach to gene therapy using Sleeping Beauty to genetically modify clinical-grade T cells to target CD19. Immunol Rev. 2014 Jan;257(1):181-90. doi: 10.1111/imr.12137. Review. PubMed PMID: 24329797; PubMed Central PMCID: PMC4109051.

2013

Singh H, Figliola MJ, Dawson MJ, Olivares S, Zhang L, Yang G, Maiti S, Manuri P, Senyukov V, Jena B, Kebriaei P, Champlin RE, Huls H, Cooper LJ. Manufacture of clinical-grade CD19-specific T cells stably expressing chimeric antigen receptor using Sleeping Beauty system and artificial antigen presenting cells. PLoS One. 2013;8(5):e64138. doi: 10.1371/journal.pone.0064138. Print 2013. PubMed PMID: 23741305; PubMed Central PMCID: PMC3669363.

Jena B, Maiti S, Huls H, Singh H, Lee DA, Champlin RE, Cooper LJ. Chimeric antigen receptor (CAR)-specific monoclonal antibody to detect CD19-specific T cells in clinical trials. PLoS One. 2013;8(3):e57838. doi: 10.1371/journal.pone.0057838. Epub 2013 Mar 1. PubMed PMID: 23469246; PubMed Central PMCID: PMC3585808.

Huls MH, Figliola MJ, Dawson MJ, Olivares S, Kebriaei P, Shpall EJ, Champlin RE, Singh H, Cooper LJ. Clinical application of Sleeping Beauty and artificial antigen presenting cells to genetically modify T cells from peripheral and umbilical cord blood. J Vis Exp. 2013 Feb 1;(72):e50070. doi: 10.3791/50070. PubMed PMID: 23407473; PubMed Central PMCID: PMC3596954.

Maiti SN, Huls H, Singh H, Dawson M, Figliola M, Olivares S, Rao P, Zhao YJ, Multani A, Yang G, Zhang L, Crossland D, Ang S, Torikai H, Rabinovich B, Lee DA, Kebriaei P, Hackett P, Champlin RE, Cooper LJ. Sleeping beauty system to redirect T-cell specificity for human applications. J Immunother. 2013 Feb;36(2):112-23. doi: 10.1097/CJI.0b013e3182811ce9. PubMed PMID: 23377665; PubMed Central PMCID: PMC3568214.

Deniger DC, Switzer K, Mi T, Maiti S, Hurton L, Singh H, Huls H, Olivares S, Lee DA, Champlin RE, Cooper LJ. Bispecific T-cells expressing polyclonal repertoire of endogenous γδ T-cell receptors and introduced CD19-specific chimeric antigen receptor. Mol Ther. 2013 Mar;21(3):638-47. doi: 10.1038/mt.2012.267. Epub 2013 Jan 8. PubMed PMID: 23295945; PubMed Central PMCID: PMC3589159.

2012

Denman CJ, Senyukov VV, Somanchi SS, Phatarpekar PV, Kopp LM, Johnson JL, Singh H, Hurton L, Maiti SN, Huls MH, Champlin RE, Cooper LJ, Lee DA. Membrane-bound IL-21 promotes sustained ex vivo proliferation of human natural killer cells. PLoS One. 2012;7(1):e30264. doi: 10.1371/journal.pone.0030264. Epub 2012 Jan 18. PubMed PMID: 22279576; PubMed Central PMCID: PMC3261192.

2011

Singh H, Figliola MJ, Dawson MJ, Huls H, Olivares S, Switzer K, Mi T, Maiti S, Kebriaei P, Lee DA, Champlin RE, Cooper LJ. Reprogramming CD19-specific T cells with IL-21 signaling can improve adoptive immunotherapy of B-lineage malignancies. Cancer Res. 2011 May 15;71(10):3516-27. doi: 10.1158/0008-5472.CAN-10-3843. Epub 2011 May 10. PubMed PMID: 21558388; PubMed Central PMCID: PMC3096697.

Jin Z, Maiti S, Huls H, Singh H, Olivares S, Mátés L, Izsvák Z, Ivics Z, Lee DA, Champlin RE, Cooper LJ. The hyperactive Sleeping Beauty transposase SB100X improves the genetic modification of T cells to express a chimeric antigen receptor. Gene Ther. 2011 Sep;18(9):849-56. doi: 10.1038/gt.2011.40. Epub 2011 Mar 31. PubMed PMID: 21451576; PubMed Central PMCID: PMC4083583.

2010

Davies JK, Singh H, Huls H, Yuk D, Lee DA, Kebriaei P, Champlin RE, Nadler LM, Guinan EC, Cooper LJ. Combining CD19 redirection and alloanergization to generate tumor-specific human T cells for allogeneic cell therapy of B-cell malignancies. Cancer Res. 2010 May 15;70(10):3915-24. doi: 10.1158/0008-5472.CAN-09-3845. Epub 2010 Apr 27. PubMed PMID: 20424114; PubMed Central PMCID: PMC2873153.

Manuri PV, Wilson MH, Maiti SN, Mi T, Singh H, Olivares S, Dawson MJ, Huls H, Lee DA, Rao PH, Kaminski JM, Nakazawa Y, Gottschalk S, Kebriaei P, Shpall EJ, Champlin RE, Cooper LJ. piggyBac transposon/transposase system to generate CD19-specific T cells for the treatment of B-lineage malignancies. Hum Gene Ther. 2010 Apr;21(4):427-37. doi: 10.1089/hum.2009.114. PubMed PMID: 19905893; PubMed Central PMCID: PMC2938363.

Hurton LV, Siddik RI, Singh H, Olivares S, Rabinovich BA, Tian R, Mojsilovic D, Hildebrand W, Lee DA, Kelly SS, Champlin R, Shpall EJ, Fernandez-Viña M, Cooper LJ. Identifying candidate allogeneic NK-cell donors for hematopoietic stem-cell transplantation based on functional phenotype. Leukemia. 2010 May;24(5):1059-62. doi: 10.1038/leu.2010.19. Epub 2010 Feb 18. PubMed PMID: 20164852; PubMed Central PMCID: PMC4135727.

2009

Singh H, Najjar AM, Olivares S, Nishii R, Mukhopadhyay U, Alauddin M, Manuri PR, Huls H, Lee DA, Dotti G, Bollard C, Simmons PJ, Shpall EJ, Champlin RE, Gelovani JG, Cooper LJ. PET imaging of T cells derived from umbilical cord blood. Leukemia. 2009 Mar;23(3):620-2. doi: 10.1038/leu.2008.256. Epub 2008 Oct 2. PubMed PMID: 18830258; PubMed Central PMCID: PMC5108177.

2008

Singh H, Manuri PR, Olivares S, Dara N, Dawson MJ, Huls H, Hackett PB, Kohn DB, Shpall EJ, Champlin RE, Cooper LJ. Redirecting specificity of T-cell populations for CD19 using the Sleeping Beauty system. Cancer Res. 2008 Apr 15;68(8):2961-71. doi: 10.1158/0008-5472.CAN-07-5600. PubMed PMID: 18413766; PubMed Central PMCID: PMC2424272.

2007

Chu S, Li L, Singh H, Bhatia R. BCR-tyrosine 177 plays an essential role in Ras and Akt activation and in human hematopoietic progenitor transformation in chronic myelogenous leukemia. Cancer Res. 2007 Jul 15;67(14):7045-53. doi: 10.1158/0008-5472.CAN-06-4312. PubMed PMID: 17638918.

Singh H, Serrano LM, Pfeiffer T, Olivares S, McNamara G, Smith DD, Al-Kadhimi Z, Forman SJ, Gillies SD, Jensen MC, Colcher D, Raubitschek A, Cooper LJ. Combining adoptive cellular and immunocytokine therapies to improve treatment of B-lineage malignancy. Cancer Res. 2007 Mar 15;67(6):2872-80. doi: 10.1158/0008-5472.CAN-06-2283. PubMed PMID: 17363611.

2006

Numbenjapon T, Serrano LM, Singh H, Kowolik CM, Olivares S, Gonzalez N, Chang WC, Forman SJ, Jensen MC, Cooper LJ. Characterization of an artificial antigen-presenting cell to propagate cytolytic CD19-specific T cells. Leukemia. 2006 Oct;20(10):1889-92. doi: 10.1038/sj.leu.2404329. PubMed PMID: 17041638.

2004

Ramaraj P, Singh H, Niu N, Chu S, Holtz M, Yee JK, Bhatia R. Effect of mutational inactivation of tyrosine kinase activity on BCR/ABL-induced abnormalities in cell growth and adhesion in human hematopoietic progenitors. Cancer Res. 2004 Aug 1;64(15):5322-31. doi: 10.1158/0008-5472.CAN-03-3656. PubMed PMID: 15289338.

2003

Singh H, Aggarwal R, Singh RL, Naik SR, Naik S. Frequency of infection by hepatitis B virus and its surface mutants in a northern Indian population. Indian J Gastroenterol. 2003 Jul-Aug;22(4):132-7. PubMed PMID: 12962435.

Agarwal N, Naik S, Aggarwal R, Singh H, Somani SK, Kini D, Pandey R, Choudhuri G, Saraswat VA, Naik SR. Occult hepatitis B virus infection as a cause of cirrhosis of liver in a region with intermediate endemicity. Indian J Gastroenterol. 2003 Jul-Aug;22(4):127-31. PubMed PMID: 12962434.

Singh H, Pradhan M, Singh RL, Phadke S, Naik SR, Aggarwal R, Naik S. High frequency of hepatitis B virus infection in patients with beta-thalassemia receiving multiple transfusions. Vox Sang. 2003 May;84(4):292-9. doi: 10.1046/j.1423-0410.2003.00300.x. PubMed PMID: 12757503.