What is the turnaround time?
The typical timeline for the complete 16S, metagenomic and bacterial RNA sequencing process is approximately 3-6 weeks, depending on the number of samples and the submission queue. Additionally, our core offers a 2-week rapid turnaround for 16S sequencing. Services are provided on a "first-come, first-served" basis, so we highly encourage you to submit your request through iLab and send your samples to our Microbiome core facility when you have samples ready.
How do I speed up the turnaround time for my order?
For 16S V4 Sequencing, you can choose the "expedited sequencing (2-week)" option in iLab. If you require expedited metagenomic and RNA sequencing, please contact the microbiome core for further information and timeline.
How much volume or how many milligrams of my samples should I submit?
Mouse stool: 2 pellets
Human stool: 200 mg or 200 μL
Urine and oral rinse: 1-2 mL
Buccal swabs: 2 mL tube with 700- 800 μL of suspension
Frozen tissue: at least 100 mg. If you have samples preserved in OMNIgene line of products, we kindly request you to contact us directly or visit the DNA Genotek website.
If I’m interested in the aliquot service, how should I submit my samples?
You may submit your samples in specimen containers, Omnigene kits, falcon, Eppendorf or 2D tubes.
How many samples do I need to submit for protocol development and optimization?
Three to five extra samples need to be submitted in order to develop or optimize a protocol.
What happens when my sample could not be amplified successfully and therefore, not sequenced?
The core personnel will let you know and only the DNA extraction fee will be applied, not the sequencing fee.
After completion, what information and data can I expect to receive from the Core?
This depends on the data analysis service requested however, for 16S V4 or V1-V3 sequencing you will receive an alpha diversity table, beta-diversity table and stacked bar plots of bacterial taxonomy. We also provide fastq files for Sequence Read Archive (SRA) repository.
When can I bring in or pick up my samples?
You may drop off or courier your samples Monday to Friday from 8:00 am to 4:00 pm. We also offer a sample pick up service from facilities and clinics across the institution in the same business hours. Please contact us to obtain the courier form.
Will I be contacted when my order is done?
Yes, you will receive an email from the core facility personnel stating that your order has been completed along with sequencing data.
I am ready to bring my samples to the lab. What do I need to make sure I have done first?
Make sure you have created an iLab account, submitted your request, provided chartstring(s) you would like to use to fund the charges, stored your samples in adequate containers and contacted us for samples submission.
What is a log sheet in iLab and how do I get one?
A log sheet is a table that includes the specifics (experiment ID, sample IDs, descriptions, container type and sample type) of your order which you must fill out before you submit your order. Once a requested has been initiated, a blank log sheet will be automatically generated for you to complete. You may download it in the form of an Excel document, fill it out, save it as a *.csv or *.xlsx. and attach it in iLab.
Will I get my sample back or will Core store samples submitted?
Expect all of the sample(s) to be utilized. The core does not store samples after sequencing is complete, only DNA.