Science Images
Lack of Crumbs gives a fly a big head.
Crumbs mutant clones mislocalize Expanded (A-C & G-J) and affect Expanded and PatJ localization in boundaries with neighboring wild type cells (D-F).
Hippo signalling regulates Merlin and Expanded expression in a tissue independent manner. (a-b’) wtsx1 mutant clones in eye-antennal imaginal discs marked by the absence of GFP (green in a’ and b’), have increased levels of Ex (grayscale in a, red in a’) and Mer (grayscale in b, red in b’) proteins. Upregulation of Ex and Mer is especially prominent in the morphogenetic furrow. (c, c’) Higher levels of Ex protein (grayscale in c, red in c’) is present in savshrp1 mutant clones marked by the absence of GFP in a third instar wing imaginal disc. (d, d’) wtsx1 mutant clones (GFP negative) in a third instar wing imaginal disc upregulate Mer expression (grayscale in d, red in d’).
Ectopic expression of persistently active aPKC in Drosophila third instar larval eye discs causes defects in apical–basal polarity and tissue architecture. Transgenes were expressed in cells posterior to the morphogenetic furrow by using the UAS-GAL4 two-component system. Wild-type (a, d, g, and j), DaPKM-transgenic (b, e, h, and k), and rPKCζ*-transgenic (c, f, i, and l) eye discs stained for aPKC/aPKM (red) and Elav (green) are shown. Boxes in a–c indicate areas of magnified views in g–l. Wild-type eye disc (m and p), DaPKM-transgenic eye disc (n and q), and rPKCζ*-transgenic eye disc (o and r) stained for Pals-associated tight junction protein (Patj) are shown. Lines in planar views (m–o) indicate location of cross-section views in p–r. Anterior is to the left for all discs.