Protocols
Yeast Protocols
- Yeast Histone Preparation I
- Yeast Histone Preparation II
- Protein Extracts for SDS-PAGE
- RNA Isolation
- Smash 'n' Grab
- Transformation I
- Transformation II
Mouse Protocols
- Fixing and Embedding Embryos
- Genotyping Protocols
- Hematoxylin and Eosin Staining of Sections for Histology
- Irradiating Mouse Embryos for p53 Expression
- Isolation of DNA from Paraffin-Embedded Tissue
- Preparing E8.5 Embryos for Karyotyping
- Preparing Embryos for Telomere Restriction Fragment Analysis (TRF)
- Making Mouse Embryonic Fibroblasts (MEFs)
- RT-PCR Primer Sets (pdf)
- Tail and Yolk Sac Digests for PCR
- Whole Mount Immunohistochemistry
- Whole Mount in Situ
Bacterial Protocols
- Bacterial Media Recipes
- Colony Hybridization
- Making Competent Cells: Heat Shock and Electrocompetent
- Plasmid Miniprep
- Transformation of DNA into Bacteria: Heat Shock, Electroporation and Blue/White Selection
Cell Culture Protocols
General Protocols
DNA
- Blunting DNA Ends: Klenow and T4 DNA Polymerase
- DNA 6X Loading Dye
- Oligonucleotide Purification
- Plasmid Miniprep
- Single Strand DNA Isolation and Mutagenesis: Used with Stratagene's QuickChange® Kit
- Southern Blot
RNA
- Northern Blot Analysis - from Gel to Washes
- RNA Isolation for Small and Large Scale Samples
- RNA Precipitation
Protein
- Bradford Assay
- Far Western
- In Vitro Kinase Assay (ATM)
- Silver Staining SDS-PAGE Gels for Protein
- Westerns
- Western Extras