We use genetic and biochemical approaches in yeast to define the functions of histone modifying enzymes in vivo. Current projects focus on the following:
- The involvement of specific HDACs in repression by the Ssn6-Tup1 corepressor
- The regulation of Set1-mediated methylation of the Dam1 kinetochore protein
- Identification of non-histone substrates of lysine methyltransferases (KMTs)
- Defining nodes of regulatory cross talk between post-translational modifications in histone and non-histone proteins
Our research includes gene targeting and transgenic approaches to understand the functions of histone acetyltransferases (GCN5 and PCAF) during mouse development and in adult tissues
Tissue Culture/Cell Lines
Projects involve transfection, siRNA, chromatin immunoprecipitation and biochemical approaches to define the functions and mechanisms of regulation of histone-modifying enzymes.