MB Core Services
Automated DNA Isolation
- Genomic DNA from tissues, cell cultures or serum
Genotyping
- Standard analysis by PCR and gel electrophoresis
- PCR with fluorescent primers, fragment analysis on ABI 3130xL
- Custom primer/assay design
DNA Sequencing
- Standard analysis on ABI 3130xL
- Alternative chemistries, e.g., dGTP, dRhodamine, BigDye 1.1 and Sequence EnhanceR
- Sequence assembly, multiple sequence alignment
- Deep sequencing with Illumina HiSeq 2500 and MiSeq instruments
Quantitative Real-Time PCR and RT-PCR
- Gene expression analysis
- Custom assay design and development
- Transgenic mouse zygosity and genomic copy number analysis
- Pathway-specific PCR SuperArray
- Custom-designed chip
Microarray Services
- RNA isolation and quality check
- cDNA synthesis and labeling
- Hybridization and scanning of commercially available microRNA microarrays
- Curation/analysis of microarray expression data including detailed sample annotation
Protein Analysis
- 1D and 2D PAGE, 2D DIGE, digital differential expression analysis, 2D western blotting, iTRAQ labeling and differential protein expression profiling analysis
- Protein pre-fractionation, abundant serum/plasma protein depletion, glycopeptide enrichment/profiling
- In-gel digestion and preparation of samples for mass spectroscopy analysis
- Antibody array (pathway-specific and/or phospho-specific)
Training and Consultation Services
- Use of digital imaging equipment (gel documentation, Typhoon)
- Use of instrumentation and software for real-time PCR, DNA sequencing, fragment analysis
- Use of robotic liquid handling, spotting equipment
- Design of oligos for PCR, real-time PCR, allele-specific PCR, plasmid construction projects
- Troubleshooting and analysis of real-time PCR projects
- Use of microarray scanning software
- Use of web-based bioinformatics tools, databases and analysis software
- Technical training in DNA, RNA and protein sample preparation methods