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MB Core Services

Automated DNA Isolation

  • Genomic DNA from tissues, cell cultures or serum

Genotyping

  • Standard analysis by PCR and gel electrophoresis
  • PCR with fluorescent primers, fragment analysis on ABI 3130xL
  • Custom primer/assay design

DNA Sequencing

Quantitative Real-Time PCR and RT-PCR

  • Gene expression analysis
  • Custom assay design and development
  • Transgenic mouse zygosity and genomic copy number analysis
  • Pathway-specific PCR SuperArray
  • Custom-designed chip

Microarray Services

  • RNA isolation and quality check
  • cDNA synthesis and labeling
  • Hybridization and scanning of commercially available microRNA microarrays
  • Curation/analysis of microarray expression data including detailed sample annotation

Protein Analysis

  • 1D and 2D PAGE, 2D DIGE, digital differential expression analysis, 2D western blotting, iTRAQ labeling and differential protein expression profiling analysis
  • Protein pre-fractionation, abundant serum/plasma protein depletion, glycopeptide enrichment/profiling
  • In-gel digestion and preparation of samples for mass spectroscopy analysis
  • Antibody array (pathway-specific and/or phospho-specific)

Training and Consultation Services

  • Use of digital imaging equipment (gel documentation, Typhoon)
  • Use of instrumentation and software for real-time PCR, DNA sequencing, fragment analysis
  • Use of robotic liquid handling, spotting equipment
  • Design of oligos for PCR, real-time PCR, allele-specific PCR, plasmid construction projects
  • Troubleshooting and analysis of real-time PCR projects
  • Use of microarray scanning software
  • Use of web-based bioinformatics tools, databases and analysis software
  • Technical training in DNA, RNA and protein sample preparation methods

© 2014 The University of Texas MD Anderson Cancer Center