Instrumentation

DeltaVision OMX Blaze V4 Super-Resolution Microscope

• Modes of operation:
  
  • Structured Illumination (SIM)
  • Total Internal Reflection Fluorescence (TIRF)
  • Fluorescence Resonance Energy Transfer (FRET)
  • Deconvolution
    
  • Widefield
• Spatial resolution: ~120 nm XY and ~250 nm Z
  
• Optical Configuration “BGR”: 405 nm, 488 nm, 568 nm, 642 nm
• Optical Configuration “CYR”: 445 nm, 514 nm, 568 nm; and transmitted light
• Best for: 3-D super-resolution imaging of fixed fluorescence-labeled cells and subcellular structures, 3-D deconvolution imaging of live cells, cellular adhesion, molecular interactions (FRET), endo and exocytosis

Vutara SR-200 Single Molecule Localization Super-Resolution Microscope

• Modes of operation:
  
  • Stochastic Optical Reconstruction Microscopy (3D- STORM, 3D-dSTORM)
    
  • Photo Activated Localization Microscopy (3D-PALM)
    
  • Ground State Depletion Microscopy (3D-GSD)
    
  • Total Internal Reflection Fluorescence Microscopy (TIRF)
• Spatial resolution: up to ~20 nm XY and ~60 nm Z (sample dependent)
  
• Up to three colors
• Available lasers for excitation and activation: 405 nm, 488 nm, 561 nm and 647 nm
• Best for: 3-D super-resolution imaging of subcellular structures (cytoskeleton, membranes, chromatin, etc.) in fixed cells
  

Leica TCS SP8 Confocal Microscope with Environmental Chamber

• Modes of operation:
  
  • Conventional, point-by-point laser scanning
    
  • Fast resonant scanning for living, motile cells
    
  • Fluorescence Recovery after Photobleaching (FRAP)
    
  • Automated mosaic stitching
• Spatial resolution: ~ 250 nm XY and 600 nm Z
  
• Available laser for fluorescence excitation: 405, 442, 458, 488, 514, 561, 633 nm
• Spectral detection with Acusto-Optical Beam Splitter (AOBS)
• Sensitive Hybride Detectors (HyD)
  
• Inverted configuration
• Best for: routine imaging of fixed cells and tissues and live cells

Multiphoton (2-Photon) Microscope    

• Modes of operation:
  • Conventional 2-photon Microscopy
  • Phosphorescence Lifetime Microscopy (PLIM/FaST-PLIM)
  • Fluorescence Lifetime Microscopy (FLIM)
• Best for: Intravital imaging (including in skin, lung, brain, liver, bone, intestine lymph nodes), intravital tumor immune surveillance, imaging of cancer metastasis and invasion, intravital imaging of oxygen, thick specimen imaging (fresh tissue, tumor spheroids, tissue phantoms, clarified tissues)

4-D Image Analysis and Tracking Software: Imaris, Slidebook

• Volume Rendering and Morphometry
• Iso-Surfaces, Segmentation
• Feature detection
• Object detection and 3-D tracking
• Colocalization
• Image mathematics
• FRET analysis