Our objective is to serve as a hypothesis-generating engine for the identification of new therapeutic targets through high-throughput, and/or high content siRNA screens, utilizing either 2D monolayer or 3D Spheroid cell culture models allowing MD Anderson investigators the chance to further develop potential targets and identify previously unknown modulators of genes of interest.
Standard Operating Procedure
We perform all the steps required for a successful High Throughput screen:
- Mycoplasma testing
- Fingerprinting of the cell lines
- Growth curve
- All phases of the Assay Development to optimize the assay to a 384 well format. This is the most important phase of the screening process and the utmost care is taken to ensure that every step of the assay is optimized.
- Performing the primary screen. All are screens are performed in triplicate for the purpose of statistical significance.
- Data generation and organization in EXCELL files
- Performing the secondary validation screen.
Our libraries comprise pooled siRNA (4 different siRNA) targeting each gene.
Genome-wide Library – library of short interfering RNAs (siRNAs), directed against 21,067 predicted open reading frames.
Druggable sub-Library – library of short interfering RNAs (siRNAs), directed against 6,024 predicted open reading frames.
Kinome sub-Library – library of short interfering RNAs (siRNAs), directed against 779 predicted open reading frames.
Custom Libraries – we cherry-pick one of our genome-wide libraries to generate small custom siRNA libraries