Imaging Services, in combination with the Histology Laboratory and Pathology Services of the Research Histology, Pathology and Imaging Services Core (RHPI) provides scientists with experimental imaging support. We specialize in consultations with researchers to design a fully integrated imaging, pathology and histology protocol for animal studies. We have imaging instruments especially designed for ante-mortem in-vivo longitudinal imaging studies ranging from high-resolution X-ray equipment to sensitive micro ultrasound and in-vivo multispectral imaging equipment.
It is often desirable to investigate and document anatomical abnormalities in living mice. For example, it may be necessary to identify mice appropriate for breeding stock or to follow lesion development over time. Imaging Services has the capability to perform high-resolution ultrasound examination of mice to identify soft tissue abnormalities and high-resolution radiography to investigate both soft tissue and skeletal anomalies. Moreover, radiography can be included in necropsy protocols to identify and measure primary or secondary bone lesions in large numbers of mice. We recently acquired an IVIS Spectrum in vivo imaging system, which provides state-of-the-art bioluminescence and fluorescence imaging capabilities. The Spectrum permits rapid, quantitative, and sensitive imaging for longitudinal studies. For more information about currently available imaging equipment, see Instrumentation.
Imaging staff will assist investigators in determining the best ante mortem imaging techniques for their purposes and interpreting the results obtained. To discuss non-invasive imaging needs, please contact our staff.
Laser Capture Microdissection
Laser capture microdissection (LCM) allows the quick and easy isolation of specific cell populations using a low-power infrared (IR) laser to activate a special thermoplastic film over the cells or tissue of interest. The activated transfer film adheres to the cells that are located within the laser beam. The energy of the laser does not affect the tissue sample; the quality of nucleic acids and proteins within the sample and the cell morphology are not compromised. For the cut-and-capture technique, an ultraviolet (UV) laser is used in conjunction with the IR capture laser. The UV laser is used to cut around the region of interest, then the cells within the region of interest are captured using the IR laser. This minimizes contamination of the cells of interest during the capture process.
The ArcturusXT LCM instrument provides an automated approach to microdissection of individual cells or multi-cellular structures from slides containing tissue sections or cytological samples. Successful isolation of macromolecules from material obtained by LCM depends upon careful and rapid sample preparation and processing. Thus, we require that advance arrangements for sample preparation be made with the Histology Laboratory, where slides will be prepared, and with the Pathology and Imaging Services personnel responsible for LCM services. Either researchers or Pathology and Imaging Services personnel can perform LCM; however, if a researcher will be performing LCM, he or she must arrange to be trained by Imaging Services personnel to operate the ArcturusXT instrument.
Manu M. Sebastian D.V.M., Ph.D., DACVP, DABT, DACLAM
Director, Research Histology, Pathology and Imaging Core
Associate Professor, Epigenetics and Molecular Carcinogenesis
Carlos Perez, D.V.M., Ph.D.
Senior Research Scientist
Digital Pathology and Imaging Services
The Virginia Harris Cockrell Cancer Research Center
at the University of Texas MD Anderson Cancer Center, Science Park
Department of Epigenetics and Molecular Carcinogenesis
Mailing Address: P.O. Box 389, Smithville, Texas 78957
Physical Address: 1808 Park Road 1C, Smithville, Texas 78957