Analytical Flow Cytometry
Flow Cytometry is an important tool in all aspects of cell biology research. Many different combinations of antibody-based stains, fluorescent protein expression, and viability indicators are measured at the single cell level. The Core provides access to cytometry equipment and staff with sufficient expertise to assist researchers in developing assays. Up to 18-color analysis is available. Users with complex assays are encouraged to contact Core personnel during the planning stages of their experiments.
We use electrostatic droplet based high-speed cell sorting to isolate a wide variety of cells based on many different combinations of fluorescence markers. Several nozzle size/pressure configurations are available. Up to 16 color cell sorting is provided with a variety of options for collection vessels including various test tube sizes and multiwell plates. Cell sorting can entail isolation of single cells as well as tube-based collection of up to 6 simultaneous populations for RNASeq, culture, or many other downstream applications.
Imaging Flow Cytometry
The ImageStreamX Mark II is an imaging flow cytometer that uses CCD cameras to image cells in flow with up to 9 fluorescent channels for each cell. A number of assays have been developed for the ImageStreamX, including colocalization, nuclear translocation, apoptosis assays, Ca2+ flux assay, characterization of diffuse vs. punctate staining, assays of immune cell interactions, extracellular vesicle detection, circulating tumor cell detection, cell counts and transfection efficiency.
Custom mAb Conjugations
The Core performs conjugations primarily for monoclonal antibodies that are not commercially available as conjugates. The core can prepare 100 microgram-scale antibody conjugations with cell-free QC. Available fluorochromes include AlexaFluor Dyes (488, 647, 555, 594, etc.) and Pacific Blue. Contact Kathryn Cox at firstname.lastname@example.org for information.
Rates for MD Anderson Cancer Center Faculty
|Service||Assisted Rate||Unassisted Rate||Unit|
|Analytical Flow Cytometry||$70||$40||Hour|
|mAb Conjugations||$130||N/A||0.1mg Reaction|