Supervisor, Laboratory & Research
Patel has more than 15 years of experience in flow cytometry instrumentation and applications. Her background in flow cytometry procedures includes staining, analyzing and interpreting samples for DNA/BRDU, TUNEL, apoptosis, autophagy, fluorescent proteins, multi-parameter surface markers and high speed sorting from blood, bone marrow and solid tumors. She also has experience with immunohistochemistry and cell culture techniques, which supplements the flow techniques.
She is proficient in operation, quality control maintenance and troubleshooting of flow cytometry instrumentation and oversees the daily functions of the Flow Cytometry Core Facility. She is able to provide clients with procedures and advice to optimize their flow cytometry assays. She schedules clients, maintains inventory, provides client training on instrumentation and writes standard operating procedures as needed. Nalini is skilled with current computer applications and has extensive experience with flow cytometry software, including the new BCI Kaluza, ModFit, BD DIVA, Winlist and Summit.
Coordinator, Flow Cytometry Lab
Nicole joined the Advanced Cytometry and Sorting Facility at North Campus in June 2021 with four years of experience in flow cytometry. She performs appointment requests on the flow cytometry instruments and assists with data analysis. Additionally, she assists with core facility operations including maintenance and QC of instruments, antibody panel design and flow cytometry assay development. She is proficient in current analysis software such as FlowJo, FCS Express, Kaluza, GraphPad Prism and BD FACSDiva.
Research Assistant II
With three years of experience working with clinical flow cytometry, Nguyen specializes in using flow cytometry techniques for leukemia diagnosis, and can offer special assistance to core facility’s clients who need to identify and characterize rare leukemia population in their research. He is familiar with the entire flow cytometry pipeline and can help our users in every step of their flow experiment, from cell staining to data acquisition and analysis. Clients with cell sorting needs can employ Nguyen’s expertise with BD FACS Melody and its large nozzle system to achieve high viability while sorting for larger cells.
Duncan Mak, MPhil
Supervisor, Laboratory & Research
Duncan has years of experience with mass cytometry (suspension and imaging mass cytometry) since the first generation of CyTOF in 2012, and over 20 years of experience with multiparameter flow cytometry. He is also responsible for managing a CyTOF antibody bank of over 800 metal-tagged antibodies, supporting users across the Texas Medical Center to design panels, conjugate antibodies and analyze high-dimensional data with existing tools like cytofkit, FlowJo with plugins and other R-based analysis packages.
Senior Research Assistant
Angelique has more than 10 years of research experience in molecular biology and hematology. Her training consisted of studying cell proliferation, cell differentiation and apoptosis of murine HSPC, with years of experience in multiparameter analysis with flow cytometry. Her responsibility includes assisting clients with training, operating and analysis of CyTOF. She is also responsible for supporting clients with the operation of Isoplexis Isolight, data analysis and maintenance of the instrument.