Kalocsay Laboratory
Marian Kalocsay, Ph.D.
Principal Investigator
Areas of Research
- Cell Signaling
- Proteomics
- Tumor Microenvironment
- Cancer Biology
- Drug Development
- Immunology
- WNT Signaling
The Kalocsay Laboratory is using quantitative mass spectrometry to understand receptor signaling. We have pioneered proximity sensitive proteomics to precisely define functional interactions in situ at unprecedented temporal and spatial resolution and developed proximity phospho-proteomics to precisely follow protein phosphorylation during signaling. We plan to harness these breakthrough technologies to define receptor signaling using experimental models for tumor immune evasion in the future, with the goal of understanding disease mechanisms and developing new therapeutics.
Principal Investigator
Marian Kalocsay, Ph.D.
Assistant Professor and CPRIT Scholar
MKalocsay@mdanderson.org
Research Interests: Developing and applying cutting edge mass spectrometry approaches to identify hitherto unknown signaling proteins and ligands and resolve the cascades of localized phosphorylation events in checkpoint signaling.
Key Publications
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Paek J*, Kalocsay M*, Staus DP, Wingler L, Pascolutti R, Paulo JA, Gygi SP, Kruse AC. * equal contribution
Multidimensional Tracking of GPCR Signaling via Peroxidase-Catalyzed Proximity Labeling.
Cell. 2017 Apr 6; 169(2):338-349.
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Kalocsay M.
APEX Peroxidase-Catalyzed Proximity Labeling and Multiplexed Quantitative Proteomics.
Methods Mol Biol. 2019; 2008:41-55.
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Liu G, Papa A, Katchman AN, Zakharov SI, Roybal D, Hennessey JA, Kushner J, Yang L, Chen BX, Kushnir A, Dangas K, Gygi SP, Pitt GS, Colecraft HM, Ben-Johny M, Kalocsay M‡ & Marx SO‡. ‡ shared correspondence
Mechanism of adrenergic Ca(V)1.2 stimulation revealed by proximity proteomics.
Nature. 2020 Jan; 577(7792):695-700.
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May EA*, Kalocsay M*, Galtier D’Auriac I, Gygi SP, Nachury MV, Mick DU. * equal contribution
Time-resolved proteomic profiling of the ciliary Hedgehog response reveals that GPR161 and PKA undergo regulated co-exit from cilia.
J Cell Biol. 2021 May 3; 220(5):e202007207.
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Martin, A. P., Bradshaw, G. A., Eisert, R. J., Egan, E. D., Tveriakhina, L., Rogers, J. M., Dates, A. N., Scanavachi, G., Aster, J. C., Kirchhausen, T., Kalocsay, M., Blacklow, S. C. *co-corresponding author
A spatiotemporal Notch interaction map from plasma membrane to nucleus
Science Signaling. 2023 Aug 1; 16(796): eadg6474.
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Susa KJ, Bradshaw GA, Eisert RJ, Schilling CM, Kalocsay M, Blacklow SC, Kruse AC. *co-corresponding author
A spatiotemporal map of co-receptor signaling networks underlying B cell activation
Cell Reports. 2024 Jun 25; 43(6):114332.
Adrenaline Handbrake
Study reveals missing link in mechanisms underlying fight-or-flight response
We are grateful for the generous funding support of our sponsors:
Cancer Prevention and Research Institute of Texas
The University of Texas System Rising STARs
National Institutes of Health
MD Anderson
Join Our Lab
Our new lab at MD Anderson is looking for highly motivated postdoctoral research scientists, Ph.D. students and research assistants to elucidate cancer-relevant molecular mechanisms in immune checkpoint signaling. We apply top-notch quantitative multiplexed TMT mass spectrometry with newest instruments for proximity proteomics of receptor signaling and cells in the tumor microenvironment. Come join a great team and help find innovative possible drug targets in cancer therapy.