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DNA 6X Loading Dye

Solutions

6X Dye II

Store at RT.

  • Bromophenol Blue
  • Xylene Cyanol
  • Ficoll Type 400
  • H2O

10 ml

Store at RT.

  • 25 mg
  • 25 mg
  • 1.5 g
  • Q to 10 ml

6X Dye IV

Store at 4°C so that mold doesn't grow in the sucrose.

  • Bromophenol Blue
  • Sucrose
  • H2O

10 ml

Store at 4°C so that mold doesn't grow in the sucrose.

  • 25 mg
  • 4 g
  • Q to 10 ml

6X Dye for Cloning

Store at RT. This works well for cloning since BMB is left out and won't obscure fragments in the 200-600 bp range.

  • Orange G
  • Xylene Cyanol
  • Ficoll Type 400
  • H2O

10 ml

Store at RT. This works well for cloning since BMB is left out and won't obscure fragments in the 200-600 bp range.

  • 25 mg
  • 25 mg
  • 1.5 g
  • Q to 10 ml

Notes

  1. Xylene Cyanol runs ~4 Kb; Bromophenol Blue ~200-400 bp; Orange G at bottom of gel.
  2. 10 ml of 6X should last you several years.
  3. You can use any combination of dyes for your loading buffer -- just choose the ones that won't obscure the bands you want to see. An Orange G alone buffer, for instance, will never have a dye front that obscures your bands.

© 2014 The University of Texas MD Anderson Cancer Center