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Bacterial Media Recipes

LB

Per liter

  • 10 g Bactotryptone
  • 5 g Yeast Extract
  • 10 g NaCl
  • Adjust pH to 7.5 with 1N NaOH 0.5 g NaCl
  • Autoclave
  • Plates:
    Add 20 g/L bactoagar prior to autoclaving, and pour when media has cooled so it is still warm to the touch.

M9

Per liter

  • 50 ml 20X M9 salts*
  • 20 ml 20% Glucose
  • 1 ml 1M MgSO4
  • Add 100 ml 10X M9 salts
  • 930 ml Autoclaved Deionized H2O

TB**

**For protein expression in LB media, due to presence of lactose in tryptone.
(Sambrook et al., 1989)

Per liter

  • 900 ml Deionized H2O
  • Autoclave, cool to 60°C, and add 100 ml sterile potassium phosphate solution
  • 12 g Bactotryptone
  • Add glucose to 1% final concentration using an autoclaved 20% stock when dealing with toxic genes, or with promoters very sensitive to T7 concentration
  • 24 g Yeast Extract
  • 4 ml Glycerol

*M9 Salts

10X M9 Salts

Per liter

  • 10 g NH4Cl
  • 30 g KH2PO4
  • 60 g Na2HPO4 ·7 H2O
  • Autoclave

20X M9 Salts

  • 20 g NH4Cl
  • 60 g KH2PO4
  • 120 g Na2HPO4 ·7 H2O
  • Autoclave

Potassium Phosphate

  • 23.1 g KH2PO4
  • 125.4 g K2HPO4
  • Autoclave

 


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