Bacterial Media Recipes
LB
Per liter
- 10 g Bactotryptone
- 5 g Yeast Extract
- 10 g NaCl
- Adjust pH to 7.5 with 1N NaOH 0.5 g NaCl
- Autoclave
- Plates:
Add 20 g/L bactoagar prior to autoclaving, and pour when media has cooled so it is still warm to the touch.
M9
Per liter
- 50 ml 20X M9 salts*
- 20 ml 20% Glucose
- 1 ml 1M MgSO4
- Add 100 ml 10X M9 salts
- 930 ml Autoclaved Deionized H2O
TB**
**For protein expression in LB media, due to presence of lactose in tryptone.
(Sambrook et al., 1989)
Per liter
- 900 ml Deionized H2O
- Autoclave, cool to 60°C, and add 100 ml sterile potassium phosphate solution
- 12 g Bactotryptone
- Add glucose to 1% final concentration using an autoclaved 20% stock when dealing with toxic genes, or with promoters very sensitive to T7 concentration
- 24 g Yeast Extract
- 4 ml Glycerol
*M9 Salts
10X M9 Salts
Per liter
- 10 g NH4Cl
- 30 g KH2PO4
- 60 g Na2HPO4 ·7 H2O
- Autoclave
20X M9 Salts
- 20 g NH4Cl
- 60 g KH2PO4
- 120 g Na2HPO4 ·7 H2O
- Autoclave
Potassium Phosphate
- 23.1 g KH2PO4
- 125.4 g K2HPO4
- Autoclave