We generally provide service and collaboration on a first-come, first-served basis. Priority is given to projects in the following order: NIH funded, foundation funded, donor funded and unfunded. Outside collaborators may obtain our services at an increased charge, but MD Anderson projects will be given priority. An estimate of completion time for service, which mainly depends on the workload, will be given upon receipt of the request.
The Cancer Genomics Core Lab (Core Lab) currently provides service with the whole genome 44K long oligo array from Agilent for both gene expression and array CGH. In the past, we have provided in-house arrays including a pathway array with 1,500 genes. The genes that are involved in cell cycle, cell death, DNA repair, metastasis and signal transduction are included. The pathway array is a long oligo (70mer) based array. The oligos were selected based on the regions that share limited homology with the family members.
The investigators will provide the Core Lab with high-quality total RNA or DNA. The Core Lab research assistant will perform probe labeling, hybridization and image acquisition. Additional RNA or DNA will be requested if the experiment fails (this can happen). The Core Lab follows the protocol provided by Agilent.
Quantitative PCR Validation
The investigators provide total RNA samples. The Core Lab will order assays-on-demand from Applied Biosystems (ABI) and carry out the PCR assay and provide the investigators with a detailed report.
Protein Lysate Array Construction
The investigator provides protein lysates, and the Core Lab research assistant will construct protein lysate array and print 24 slides in each batch. Up to 96 samples are arrayed on each array.