Cancer Genomics Core Laboratory
The mission of the Cancer Genomics Core Lab is to make genomics technologies accessible to MD Anderson investigators at a reasonable cost.
Established in 1999, the Cancer Genomics Core Laboratory has implemented many core technologies: cDNA microarray, SAGE, real-time PCR, SNP assay and tissue microarray.
For microarray technology, we developed and printed a number of focused arrays with either cDNA or long oligonucleotide (70mer) arrays tailored for the different needs of specific cancer research programs. Since 2004, we have switched to the whole genome 44K arrays from Agilent, also based on long oligonucleotide (60mer), which we believe provides better specificity. High-quality results have been obtained with Agilent microarrays in several projects.
Real-time PCR assays based on Applied Biosystems' assay-on-demand are routinely used for validation purposes and for genotyping projects. The tissue microarray facility, which is headed by Dr. Gregory Fuller, chief of neuropathology and immunohistochemistry, provides an important validation of genomics studies at the protein and cell levels.
In 2004, the Cancer Genomics Core Laboratory (Core Lab) implemented the reverse phase protein lysate array technology to profile protein expression. A cell line array that contains 90 different cell lines has been made available to MD Anderson investigators. A new analysis method has been developed and a paper describing the method has been published in the journal Bioinformatics. A glioma lysate array project led to a publication highlighted on the cover of the April 2006 issue of Proteomics.
The Core Lab personnel closely interact with investigators in RNA quality control, microarray result validation and protein microarrays. Several institutional workshops and minisymposia on microarrays have been organized by the Core Lab for the benefit of investigators. We have provided support to approximately 100 MD Anderson investigators and a number of external collaborators in the areas of microarray, SAGE, SNP, real-time PCR, imaging and data analysis. The Core Lab has assisted numerous MD Anderson investigators in grant applications and has been successful in a number of cases, including the Genitourinary SPORE, R01s and R21.
The Cancer Genomics Core Laboratory is a part of the NCI-funded Cancer Center Support Grant. Core Lab researchers have published several books on genomics and many papers in the area of genomics and informatics. See our Publications page.
News and Updates
Read about lab activities, news and announcements.