Research Histology, Pathology and Imaging Core
Manu M. Sebastian, D.V.M., Ph.D., D.A.C.V.P, D.A.B.T., D.A.C.L.A.M.
Director
Research Histology, Pathology and Imaging Core
The Histology Core and Mutant Mouse Pathology Service (MMPS) have combined to form the CCSG-funded Science Park Research Histology, Pathology and Imaging Core (RHPI). The RHPI, directed by Manu M. Sebastian, D.V.M, Ph.D., D.A.C.V.P, D.A.B.T., D.A.C.L.A.M, comprises three units: 1) RHPI - Histology Laboratory, 2) RHPI - Pathology Services, and 3) RHPI - Imaging Services. These three units work together to specialize in consultations for designing integrated histology, pathology and imaging protocols for animal studies.
B cells stained within the spleen, the organ in which they develop
Hematoxylin and eosin (H&E) stained kidney cells
Whole-mount mammary gland stained with carmine red
H&E stained epithelial cells at 40x magnification
Calbindin-D staining in a cerebellum section
Trichrome (three color) staining of the heart to distinguish connective tissues
Histology Laboratory
The Histology Laboratory provides researchers with routine and specialized histology and immunohistochemistry services, technical assistance, and training and consultation in histological techniques. The Histology Laboratory has developed over 500 customized immunohistochemistry (IHC) protocols for both animal and human tissue samples, and in the last few years has served researchers from 10 different departments on three MD Anderson campuses, four UT System institutions, and clients from Texas A&M HSC, Baylor, Rice, and five institutions outside of Texas.
Pathology Services
Pathology Services, overseen by our skilled veterinary pathologist and RHPI director, Manu M. Sebastian, D.V.M, Ph.D., D.A.C.V.P, D.A.B.T., D.A.C.L.A.M., is coordinated in part by the Histology Laboratory staff. Veterinary pathologists can provide valuable insight into the comparative pathology of human and mouse diseases. Discussions with such experts during the planning stages of a study can help optimize study output; in later stages, these experts can help interpret immunohistochemical and quantitative histopathologic data. Pathology Services specializes in consultations to create collaboratively designed, fully integrated ante- and post-mortem approaches for imaging, pathology and histology studies as well as in vivo safety and efficacy protocols. Specialized pathology equipment includes a VetScan2 clinical chemistry analyzer for blood chemistry and electrolyte analysis, and a HemaVet 950S for complete blood counts.
Imaging Services
Imaging Services helps investigators design the best ante-mortem imaging approaches to meet their specific study goals, and helps investigators interpret their imaging results. They can provide scientists with specialized animal imaging services including high-resolution X-ray, senstitive micro-ultrasound, and state-of-the-art, in vivo, multispectral imaging of bioluminescent and fluorescent signals for rapid, quantitative, sensitive imaging during longitudinal studies. The core also maintains a laser-capture microdissection microscope.
Contact Us
Manu Sebastian, D.V.M, Ph.D., D.A.C.V.P, D.A.B.T., D.A.C.L.A.M.
Associate Professor, Epigenetics and Molecular Carcinogenesis
Director, Research Histology, Pathology and Imaging Core
Phone: 512-237-9347
Email: mmsebastian@mdanderson.org
The Virginia Harris Cockrell Cancer Research Center
at The University of Texas MD Anderson Cancer Center, Science Park,
Department of Epigenetics and Molecular Carcinogenesis
Mailing Address: P.O. Box 389, Smithville, Texas 78957
Physical Address: 1808 Park Road 1C, Smithville, Texas 78957