Monoclonal Antibody Facility
Laura Bover, Ph.D.
Director
- Research Resources
- Core Facilities and Services
- Monoclonal Antibody Core Facility
Overview
The Monoclonal Antibody Facility (MAF) provides newly generated custom monoclonal antibodies and purification from user's or commercially available hybridomas, plus additional services to researchers at MD Anderson and beyond. Services include:
- MAbs generation, (mice provision, antigen design, immunization, screening with proprietary methods, purification)
- Characterization, sequencing, chimera construction, isotype exchange
- Recombinant antibody transient construction
- Binding Kinetics by OCTET (bilayer interferometry)
- Epitope mapping (by overlapping peptide method)
- Providing Letters of Support or Collaboration for grant applications
- Help in designing in vitro, ex vivo, and/or in vivo validation experiments for your research
Go to the About page to learn more about MAF.
Getting Started
- Investigators wishing to use the services of the MAF should contact Long Vien (Core Manager) at lvien@mdanderson.org (713-563-3223) and/or Dr. Laura Bover (Core Director) at lbover@mdanderson.org (713-563-3301/ 73-703-1951) to set up a zoom meeting to plan the project strategy with no obligation to proceed.
- After the initial meeting and discussions about the particular project/s, a summary letter with the descriptions of procedures that we do at the Core will be sent to the PI and contacts involved in the project and/or the zoom discussion.
- Upon notification from the investigator that they would like to proceed, investigators will be guided in how to provide a completed MAF services request from iLab.
- Letters of support or collaboration and/or estimated budgets for grant applications can also be provided in advance of initiating any service order.
Impact
Monoclonal Antibodies (MAbs) have become an essential tool in biochemistry, molecular biology, and medicine. Basic, translational, and clinical researchers have been taking advantage of them for a wide range of uses, such as immunoprecipitation, immunohistochemistry, western-blotting, ELISAs, preclinical and clinical therapeutic purposes. These are all applications and protocols in which our core facility has expertise and success.
The Monoclonal Antibody Facility (MAF) provides newly generated custom monoclonal antibodies and purification, not only from own generated antibodies but also from user's or commercially available hybridomas, plus additional services for researchers at MD Anderson and beyond.
Since 2007, MD Anderson Monoclonal Antibodies Core Facility has generated more than 450 custom monoclonal antibodies (MAbs). The facility has provided services to more than 100 researchers over five years.
Acknowledgment
Please use the following text when acknowledging the core: "The Monoclonal Antibody Facility was supported in part by The University of Texas MD Anderson Cancer Center and P30CA016672"
Go to the Acknowledgment page to see guidelines on whether the efforts of core personnel merit authorship.
Contact
Monoclonal Antibody Facility
South Campus Research Building I (SCRB I)
Room SCRB4.2222 (4th floor of building I)
7455 Fannin Street, Unit 902
Houston, TX 77054
Long Vien lvien@mdanderson.org
Laura Bover lbover@mdanderson.org
Felipe Amaya FAmaya@mdanderson.org
Internal Users
The MAF now offers the generation of fully human monoclonal antibodies from scratch by hybridoma technology. Using this platform of humanized mice, which have an immune repertoire that has been genetically engineered to replicate a human immune repertoire, we can generate fully human recombinant monoclonal antibodies.
Investigators interested in this approach can contact us for further information.