Services
Our protein-domain microarray technology facilitates the identification of proteins that interact with motifs that carry phosphorylation, methylation and acetylation modifications. This high-throughput approach allows for the rapid identification of protein-protein interactions in vitro. Additional experiments are then required to confirm that these interactions do indeed occur in vivo and in vitro.
We have developed a chip-size array of proteins that can be used to identify the “readers” of specific PTMs. We are the only research group to have such a large collection of protein domains. Thus, many researchers, who have identified PTMs that are often unique signatures of a certain cancer, send us their modified proteins in the hopes that we can identify relevant binding partners. We are now expanding this facility to not only include state-of-the-art protein domain microarrays, but also to allow the further characterization of these protein-protein interactions in cells. In addition, we will provide the instrumentation and know-how to determine the strength of these protein-protein interactions.
The following table provides a guide for requirements for various kinds of experiments, the sample requirements of the Core, and estimated costs. For more detailed information, please call or e-mail to set up a meeting with Core staff. Contact Mark Bedford (mtbedford@mdanderson.org) for experiments involving the use of protein domain microarrays and surface plasmon resonance (SPR), and contact Shawn Bratton (sbbratton@mdanderson.org) for experiments involving the biophysical analysis of protein complexes.
Note: Prices are subject to change without prior notice.
External Non-Users are subject to a 60% institutional overhead charged to be accounted at billing.
Probe Protein Domain Arrays with Peptides or Small Molecules:
Description |
User Price |
Non-User Price |
User provides biotinylated peptide as probe | $80/sample | $240/sample |
User provides biotinylated small molecule as probe | $80/sample | $240/sample |
Core has peptide probe synthesized and screens an array | $750/sample | Contact Core for details** |
Array Types:
- pY-Reader - SH2 domain array for phospho-tyrosine ligand analysis.
- pS/T-Reader - BRCT, 14-3-3 & FHA domain array for phospho-threonine and -serine ligand analysis.
- Me-Reader - Tudor, Chromo, and PHD domain array for methyl-arginine and -lysine ligand analysis.
- Proline-Reader – WW and SH3 domain array for proline-rich ligand analysis.
- Sumo-Reader – An array of Sumo-interacting motifs (SIMs)
- Ac-Reader - Bromo domain array for acetylated lysine ligand analysis.
- Free C-terminal-Readers - PDZ domain array to identify free C-terminal ligands.
- Ubiquitin-Reader - An array of Ubiquitin-binding domains including UBA, UEV, GAT, VHS, UIM and CUE.
Affinity Measurements (Kd) for Protein complexes:
Description |
User Price |
Non-User Price |
User-provided pure proteins | $200/sample | $600/sample |
Core-generated pure proteins | Contact Core for details** | Contact Core for details** |
Stoichiometry of Protein Complexes by SEC-MALS*:
Description |
User Price |
Non-User Price |
User-provided pure proteins | $400/sample | $1,200/sample |
Core-generated pure proteins | Contact Core for details** | Contact Core for details** |
**Fee depends upon difficulty and time required to generate pure recombinant proteins; please contact Core for details.
Surface Plasmon Resonance (SPR) by Biacore X100:
The Biacore X100 uses Surface Plasmon Resonance to measure interactions in real-time, and can be used for characterization of kinetic and affinity properties of protein-protein interactions. Presently, two sets of kit and chip combinations are offered. Interested SPR users may request specifics on potential kit and chip combinations.
Description
|
User Price
|
Non-User Price
|
Amine Coupling Kit & CM5 Chip (3 chips) - GE Catalog #BR100050 & BR100012 |
$750/kit&chip | Contact Core for details |
SA Chip (3 chips) - GE Catalog # BR100032 | $750/kit&chip | Contact Core for details |
Contact Us
The Virginia Harris Cockrell Cancer Research Center
at the University of Texas MD Anderson Cancer Center, Science Park
Department of Epigenetics and Molecular Carcinogenesis
Mailing Address: P.O. Box 389, Smithville, Texas 78957
Physical Address: 1808 Park Road 1C, Smithville, Texas 78957
Mark Bedford, Ph.D.
Director, Protein Array and Analysis Core
Professor, Epigenetics and Molecular Carcinogenesis
512-237-9539
mtbedford@mdanderson.org
Shawn B. Bratton, Ph.D.
Co-Director, Protein Array and Analysis Core
Associate Professor, Epigenetics and Molecular Carcinogenesis
512-237-9461
sbbratton@mdanderson.org