Our protein-domain microarray technology facilitates the identification of proteins that interact with motifs that carry phosphorylation, methylation and acetylation modifications. This high-throughput approach allows for the rapid identification of protein-protein interactions in vitro. Additional experiments are then required to confirm that these interactions do indeed occur in vivo and in vitro.

We have developed a chip-size array of proteins that can be used to identify the “readers” of specific PTMs. We are the only research group to have such a large collection of protein domains. Thus, many researchers, who have identified PTMs that are often unique signatures of a certain cancer, send us their modified proteins in the hopes that we can identify relevant binding partners. We are now expanding this facility to not only include state-of-the-art protein domain microarrays, but also to allow the further characterization of these protein-protein interactions in cells. In addition, we will provide the instrumentation and know-how to determine the strength of these protein-protein interactions.

The following table provides a guide for requirements for various kinds of experiments, the sample requirements of the Core, and estimated costs.  For more detailed information, please call or e-mail to set up a meeting with Core staff. Contact Mark Bedford (mtbedford@mdanderson.org) for experiments involving the use of protein domain microarrays and surface plasmon resonance (SPR), and contact Shawn Bratton (sbbratton@mdanderson.org) for experiments involving the biophysical analysis of protein complexes.

Note:  Prices are subject to change without prior notice.
External Non-Users are subject to a 60% institutional overhead charged to be accounted at billing.

Probe Protein Domain Arrays with Peptides or Small Molecules: 

Array Types:

• pY-Reader - SH2 domain array for phospho-tyrosine ligand analysis.
• pS/T-Reader - BRCT, 14-3-3 & FHA domain array for phospho-threonine and -serine ligand analysis.
• Me-Reader - Tudor, Chromo, and PHD domain array for methyl-arginine and -lysine ligand analysis.
• Proline-Reader – WW and SH3 domain array for proline-rich ligand analysis.
• Sumo-Reader – An array of Sumo-interacting motifs (SIMs)
• Ac-Reader - Bromo domain array for acetylated lysine ligand analysis. 
• Free C-terminal-Readers - PDZ domain array to identify free C-terminal ligands. 
• Ubiquitin-Reader - An array of Ubiquitin-binding domains including UBA, UEV, GAT, VHS, UIM and CUE.
  

Affinity Measurements (Kd) for Protein complexes:

Stoichiometry of Protein Complexes by SEC-MALS*:

**Fee depends upon difficulty and time required to generate pure recombinant proteins; please contact Core for details.

Surface Plasmon Resonance (SPR) by Biacore X100:

The Biacore X100 uses Surface Plasmon Resonance to measure interactions in real-time, and can be used for characterization of kinetic and affinity properties of protein-protein interactions. Presently, two sets of kit and chip combinations are offered. Interested SPR users may request specifics on potential kit and chip combinations.