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Services

MAb development

Procurement of mice
Mouse and Rat immunization protocols for whole cells, purified proteins, peptides and other antigens
Antibodies for IHC, WB, IP, functional, blocking, agonist, anti-idiotype. For in vitro, ex vivo, or in vivo experiments, and as proof of concepts for potential therapeutic use
Antigen sources: human, mouse, elephant, dog, fungus, and other proteins from other species
Use of regular mice, KO mice, or "Humice" (to generate fully human antibodies upon recombinant construction)
Testing mouse/rat sera for positive response

Cell Fusion of immunized mouse/rat spleen or lymph node cells with drug-sensitive myeloma fusion partner for generation of hybridomas
Growing and isolation of primary hybrid fused cells by tissue culture
Screening by ELISA
Provide investigators with culture supernatants for antibody assays and validation with their own protocols
Special screening by FACS in collaboration with Flow Cytometry Lab South Campus (Karen Ramirez, kmramirez@mdanderson.org)
Special screening for immunohistochemistry antibodies in collaboration with the Histology Core (Dr. Gabriela Raso, graso@mdanderson.org)

Single cell cloning of desired hybridomas to create a stable cell line
Freezing representative hybridoma cell lines for long term viable storage in our liquid nitrogen tanks at no extra charge

Production of antibody-containing culture supernatants (2 mLs), to provide the Core user with all the positive clones for further analysis and selection in their labs
We support our users all the way through the selection providing all results to users

Small/medium scale production of purified monoclonal antibodies from custom hybridoma supernatants or hybridomas stocked in the MAF's extensive library
Protein A/ Protein G affinity chromatography purification
Affinity Purification of monoclonal antibodies using FPLC (Fast Protein Liquid Chromatography)
Purification from mice ascites fluid
In vitro purification from non-murine hybridomas, recombinant CHO and/or 293-HEK cells
Large scale purification of antibodies for in vivo experiments (information provided to contact the recommended company)

Murine Isotyping
SDS PAGE gel electrophoresis
F(ab) and F(ab)₂ fragment production
Sequencing and provision of a comprehensive report suitable for patent applications

Fluorochrome labeled antibodies in collaboration with Flow Cytometry Lab South Campus (Karen Ramirez, kmramirez@mdanderson.org)
Biotin labeling

Isotype exchange for triggering different functions
Chimera construction (mouse variable – human constant)
Design of stable master cell lines in recombinant Expi-CHO and/or Expi-293-HEK cells
Generation of Master Cell Lines of the recombinant antibodies best candidates in collaboration with Functional Genomics Core (Dr. Yutong Sun, YSun2@mdanderson.org)
Production of stable cell line expressing the target of interest in collaboration with Functional Genomics Core (Dr. Yutong Sun, YSun2@mdanderson.org)

See the About page for examples of antibodies we produced and patents granted.