Services
mAb development
- Procurement of mice
- Mouse and Rat immunization protocols for whole cells, purified proteins, peptides and other antigens
- Testing mouse/rat sera for positive response
Fusion and Hybridoma Screening
- Cell Fusion of immunized mouse spleen or lymph node cells with drug-sensitive myeloma fusion partner for generation of hybridomas
- Growing and isolation of primary fusants by tissue culture
- Screening done by ELISA
- Provide investigators with culture supernatants for antibody assays
- Special screening by FACS
Cloning
- Single cell cloning of desired hybridomas to create stable cell line
- Freezing representative hybridoma cell lines for long term viable storage in liquid nitrogen
Small-scale antibody production
- Production of large amounts of antibody-containing culture supernatants or mice ascites fluid
- In vitro production for non-murine hybridomas
Antibody purification
- Small scale production of purified monoclonal antibodies from custom hybridomas or hybridomas stocked under MABF extensive library
- Protein A/ Protein G affinity chromatography
- Affinity Purification of monoclonal antibodies using FPLC (Fast Protein Liquid Chromatography)
Antibody characterization
- Murine Isotyping
- SDS PAGE gel electrophoresis
- F(ab) and F(ab)2 fragment production
Antibody conjugation
- Fluorochromes
- Biotin
Examples of Antibodies Produced
Cell Surface Proteins
- TSLP
- TSLP-receptor
- C5a-receptor
- C3a-receptor
- ICOS-Ligand
- OX40-Ligand
- CD49e
- CD19-receptor
- CD11a high affinity
- VCAM
- ILT7 (immunoglobulin like transcript 7)
- BST2 (bone marrow stromal cell antigen 2)
- OX40
- Pacsin
- IL-25R
- N100sAd24
- PTPRS
- IL-13Ra1
- CD70
- Pd-1
- Sema4a
- PRMT9
- MLCN2
- EphA2
- Ngal
- IL-26
Peptides
- 8A8-LL37 (antimicrobial peptide)
- PR1/HLA-A2 (antigen aberrantly expressed in myeloid leukemia)
Intracellular Proteins
- MPP8
- HA4
- MiP
- CiP4
- MEKK2
Extracellular Matrix
- DSP
- BSP
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