Services

mAb development

• Procurement of mice
• Mouse and Rat immunization protocols for whole cells, purified proteins, peptides and other antigens
• Testing mouse/rat sera for positive response

Fusion and Hybridoma Screening

• Cell Fusion of immunized mouse spleen or lymph node cells with drug-sensitive myeloma fusion partner for generation of hybridomas
• Growing and isolation of primary fusants by tissue culture
• Screening done by ELISA
• Provide investigators with culture supernatants for antibody assays
• Special screening by FACS

Cloning

• Single cell cloning of desired hybridomas to create stable cell line
• Freezing representative hybridoma cell lines for long term viable storage in liquid nitrogen

Small-scale antibody production

• Production of large amounts of antibody-containing culture supernatants or mice ascites fluid
• In vitro production for non-murine hybridomas

Antibody purification

• Small scale production of purified monoclonal antibodies from custom hybridomas or hybridomas stocked under MABF extensive library
• Protein A/ Protein G affinity chromatography
• Affinity Purification of monoclonal antibodies using FPLC (Fast Protein Liquid Chromatography)

Antibody characterization

• Murine Isotyping
• SDS PAGE gel electrophoresis
• F(ab) and F(ab)₂ fragment production

Antibody conjugation

• Fluorochromes
• Biotin

Examples of Antibodies Produced