MAP2K1, otherwise known as Mitogen-Activated Protein Kinase Kinase 1, is located on chromosome 15q22, and encodes for a serine/thronine kinase involved in numerous cellular processes such as proliferation, differentiation, transcriptional regulation, as well as roles in development. Mutations in exons 2 and 3 of MAP2K1 are rare, but have been described in melanoma, non-small cell lung carcinoma and in colorectal adenocarcinoma and have been shown to confer resistance to both RAF and MAP2K1 inhibition in vitro, however other mutations in MAP2K1 may also indicate sensitivity to certain MAP2K1 inhibitors, thereby necessitating accurate profiling of MAP2K1 mutations in tumors. Mutational profiling of MAP2K1 is provided through a mutational analysis assay developed and validated at MDL. MAP2K1 mutation analysis is carried out via PCR followed by bi-directional DNA (Sanger) sequencing.
PCR-based DNA Sanger sequencing.
This assay will detect mutations in exons 2 and 3 of the MAP2K1 gene. The sensitivity of detection is approximately one mutated cell per twenty cells in the sample.
Formalin-fixed, paraffin embedded tissue blocks containing adequate amounts of tumor to be analyzed (see above sensitivity), with areas to be tested indicated.
Please provide a copy of the corresponding pathology report.
83890 (DNA Isolation), 83898x2, 83904x2, 83912
Additional charges may apply for tissue extraction.
The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.