South Campus Instrumentation
FACSAria Fusion Cell Sorter*
Optics/Configurations: 6-laser, 15-color
Laser: 407
Filter 450/50 BP:
Alexa Fluor405, DAPI, Marina Blue, Pacific Blue, eFluor450, Sytox Blue, Calcien Blue, BV421
Filter 525/50 BP:
Alexa Fluor430, AmCyan, CFP, Live Dead fixable Aqua, BV510
Filter 610/20 BP:
BV605, Pacific Orange, Qdot 605
Filter 660/20 BP:
BV650, Qdot 655
Filter 710/50 BP:
BV711, Qdot 700
Filter 780/60 BP:
BV786, Qdot 800
Laser: 488
Filter 530/30 BP:
Alexa Fluor488, FITC, GFP, Sytox Green, Calcien Green
Filter 695/40 BP:
PE-Cy5, PerCP, PerCP-Cy5, PerCP-Cy5.5, PI, 7AAD
Laser: 561
Filter 582/15 BP:
PE, DS Red
Filter 610/20 BP:
PE-Texas Red, Alexa Fluor610, mCherry, mKate, tdTomato
Filter 670/14 BP:
PE-Cy5
Filter 780/60 BP:
PE-Cy7
Laser: 633
Filter 670/30 BP:
APC, Alexa Fluor647, Sytox Red
Filter 730/45 BP:
Alexa Fluor700
Filter 780/60 BP:
APC-Cy7, APC-H7
Lasers 375 and 445 additionally available. Contact flow staff for more information.
Software Version: BD FACSDiva 8.0
Sample Tube: 12mm x 75mm tube
Sample Collection: 15ml tubes (up to two populations), 12mm x 75mm tubes (up to four populations), 1.5ml microcentrifuge tubes (up to four populations), 24, 96, 384 well plates
Nozzle/Pressure: 70um/70psi (default), 100um/30psi (by request; call flow staff for more information)
*Class II Biosafety Cabinet
Influx Cell Sorter
Optics/Configurations: 5-laser, 14-color
Laser: 355
Filter 460/50 BP:
Hoechst Blue, DAPI
Filter 670/30 BP:
Hoechst Red
Laser: 405
Filter 460/50 BP:
Alexa Fluor405, Marina Blue, Pacific Blue, eFluor450, Sytox Blue, Calcien Blue, BV421
Filter 520/35 BP:
Alexa Fluor430, AmCyan, CFP, Live Dead fixable Aqua, BV510
Laser: 488
Filter 530/40 BP:
Alexa Fluor488, FITC, GFP, Sytox Green, Calcien Green
Filter 670/30 BP:
PerCP, PI, 7AAD
Filter 710/50 BP:
PerCP-Cy5, PerCP-Cy5.5
Laser: 561
Filter 585/29 BP:
PE, DS Red
Filter 610/20 BP:
PE-Texas Red, Alexa Fluor610, mCherry, mKate, tdTomato
Filter 670/30 BP:
PE-Cy5
Filter 750 LP:
PE-Cy7
Laser: 642
Filter 670/30 BP:
APC, Alexa Fluor647, Sytox Red
Filter 720/40 BP:
Alexa Fluor700
Filter 750 LP:
APC-Cy7, APC-H7
Software Version: BD Sortware 1.2.0.142
Sample Tube: 12mm x 75mm polypropylene tube
Sample Collection: 15ml tubes (up to two populations), 12mm x 75mm tubes (up to four populations), 50ml tube (up to two populations), 1.5ml microcentrifuge tubes (up to six populations), 24, 96, 384 well plates
Nozzle/Pressure: 100um/27 psi (default), 70um/52psi (by request; call flow staff for more information)
FACSAria IIIu #1 Cell Sorter
Optics/Configurations: 4-laser, 14-color
Laser: 405
Filter 450/50 BP:
Alexa Fluor 405, DAPI, Marina Blue, Pacific Blue, eFluor450, Sytox Blue, Calcien Blue, BV421
Filter 530/30 BP:
Alexa Fluor 430, AmCyan, CFP, Live Dead fixable Aqua, BV510
Filter 610/20 BP:
BV605, Pacific Orange, Qdot 605
Filter 670/30 BP:
BV650, Qdot 655
Filter 710/40 BP:
BV711, Qdot 700
(Filter 780/60 BP for BV786 Available Upon Request)
Laser: 488
Filter 530/30 BP:
Alexa Fluor 488, FITC, GFP, Sytox Green, Calcien Green
Filter 695/40 BP:
PerCP, PerCP-Cy5, PerCP-Cy5.5, 7AAD
Laser: 561
Filter 582/15 BP:
PE, DS Red
Filter 610/20 BP:
PI, PE-Texas Red, Alexa Fluor 610, mCherry, mKate, tdTomato
Filter 670/40 BP:
PE-Cy5
Filter 780/60 BP:
PE-Cy7
Laser: 633
Filter 660/20 BP:
APC, Alexa Fluor 647, Sytox Red
Filter 720/40 BP:
Alexa Fluor 700
Filter 780/60 BP:
Alexa Fluor 700, APC-Cy7, APC-H7
Software Version: BD FACSDiva 8.0.1
Sample Tube: 12mm x 75mm tube
Sample Collection: 15ml tubes (up to two populations), 12mm x 75mm tubes (up to four populations), 1.5ml microcentrifuge tubes (up to four populations), 24, 96, 384 well plates
Nozzle/Pressure: 70um/70psi (default), 100um/30psi (by request; call flow staff for more information)
FACSAria IIIu #2 Cell Sorter
Optics/Configurations: 4-laser, 16-color
Laser: 405
Filter 450/40 BP:
Alexa Fluor 405, DAPI, Marina Blue, Pacific Blue, eFluor450, Sytox Blue, Calcien Blue, BV421
Filter 530/30 BP:
Alexa Fluor 430, AmCyan, CFP, Live Dead Fixable Aqua, BV510
Filter 575/25 BP:
Pacific Orange, BV570
Filter 610/20 BP:
BV605, Qdot 605
Filter 670/30 BP:
BV650, Qdot 655
Filter 710/40 BP:
BV711, Qdot 700
Filter 780/60 BP:
BV786
Laser: 488
Filter 530/30 BP:
Alexa Fluor488, FITC, GFP, Sytox Green, Calcien Green
Filter 695/40 BP:
PerCP, PerCP-Cy5, PerCP-Cy5.5, 7AAD
Laser: 561
Filter 582/15 BP:
PE, DS Red
Filter 610/20 BP:
PI, PE-Texas Red, AlexaFluor 610, mCherry, mKate, tdTomato
Filter 670/40 BP:
PE-Cy5
Filter 780/60 BP:
PE-Cy7
Laser: 640
Filter 660/20 BP:
APC, Alexa Fluor647, Sytox Red
Filter 780/60 BP:
Alexa Fluor700, APC-Cy7, APC-H7
Software Version: BD FACSDiva 8.0.1
Sample Tube: 12mm x 75mm tube
Sample Collection: 15ml tubes (up to two populations),12mm x 75mm tubes (up to four populations),1.5ml microcentrifuge tubes (up to four populations)
Nozzle/Pressure: 70um/70psi (default), 100um/30psi (by request; call flow staff for more information)
FACSJazz Cell Sorter*
Optics/Configurations: 2-laser, 6-color
Laser: 488
Filter 530/40 BP:
Alexa Fluor488, FITC, GFP, Sytox Green, Calcien Green
Filter 576/26 BP:
PE, DS Red
Filter 629/40 BP:
PerCP, PerCP-Cy5, PerCP-Cy5-5, PI, 7AAD
Filter 750 LP:
PE-Cy7
Laser: 640
Filter 670/30 BP:
APC, Alexa Fluor647, Sytox Red
Filter 750 LP:
APC-Cy7, APC-H7
Software Version: BD SortWare 1.2.0.142
Sample Tube: 12mm x 75mm polypropylene tube
Sample Collection: 15ml tubes (up to two populations), 12mm x 75mm tubes (up to four populations), 50ml tube (up to two populations), 1.5ml microcentrifuge tubes (up to four populations), 24, 96, 384 well plates
Nozzle/Pressure: 100um/27psi (fixed)
*class II biosafety cabinet
LSRFortessa X-20 Cell Analyzer (Right)
Optics/Configurations: 5-laser, 18-color
Laser: 355
Filter 379/28 BP:
Hoechst Blue, BUV396
Filter 740/35 BP:
BUV737
Laser: 405
Filter 450/50 BP:
Alexa Fluor 405, Marina Blue, Pacific Blue, eFluor450, Sytox Blue, Calcien Violet, BV421, DAPI
Filter 525/50 BP:
Alexa Fluor 430, AmCyan, CFP, Live/Dead fixable Aqua, BV510
Filter 610/20 BP:
BV605, Pacific Orange, Qdot 605
Filter 670/30 BP:
BV650, Qdot 655
Filter 710/50 BP:
BV711, Qdot 700
Filter 780/60 BP:
BV786, Qdot 800
Laser: 488
Filter 530/30 BP:
Alexa Fluor 488, FITC, GFP, Sytox Green, Calcien Green
Filter 710/50 BP:
PerCP, PerCP-Cy5, PerCP-Cy5.5, 7-AAD
Laser: 561
Filter 586/15 BP:
PE, DS Red, PI
Filter 610/20 BP:
PE-Texas Red, PE-Alexa 610, PE-CF594, Live/Dead Fixable Red, Alexa Fluor 610, mCherry, mKate, tdTomato
Filter 670/30 BP:
PE-Cy5
Filter 710/50 BP:
PE-Cy5.5, PE-Alexa 700
Filter 780/60 BP:
PE-Cy7
Laser: 640
Filter 670/30 BP:
APC, Alexa Fluor 647, Alexa Fluor 680, Sytox Red
Filter 730/45 BP:
Alexa Fluor 700, APC-Alexa 700, APC-R700
Filter 780/60 BP:
APC-Cy7, APC-H7
Software Version: BD FACSDiva 8.0
Sample Acquisition: 12mm x 75mm polystyrene tubes, HTS plate reader (96, 384 well plates)
LSRFortessa X-20 Cell Analyzer (Left)
Optics/Configurations: 5-laser, 18-color
Laser: 355
Filter 379/28 BP:
Hoechst Blue, BUV396
Filter 740/35 BP:
BUV737
Laser: 405
Filter 450/50 BP:
Alexa Fluor 405, Marina Blue, Pacific Blue, eFluor450, Sytox Blue, Calcien Violet, BV421, DAPI
Filter 525/50 BP:
Alexa Fluor 430, AmCyan, CFP, Live/Dead fixable Aqua, BV510
Filter 610/20 BP:
BV605, Pacific Orange, Qdot 605
Filter 670/30 BP:
BV650, Qdot 655
Filter 710/50 BP:
BV711, Qdot 700
Filter 780/60 BP:
BV786, Qdot 800
Laser: 488
Filter 530/30 BP:
Alexa Fluor 488, FITC, GFP, Sytox Green, Calcien Green
Filter 710/50 BP:
PerCP, PerCP-Cy5, PerCP-Cy5.5, 7-AAD
Laser: 561
Filter 586/15 BP:
PE, DS Red, PI
Filter 610/20 BP:
PE-Texas Red, PE-Alexa 610, PE-CF594, Live/Dead Fixable Red, Alexa Fluor 610, mCherry, mKate, tdTomato
Filter 670/30 BP:
PE-Cy5
Filter 710/50 BP:
PE-Cy5.5, PE-Alexa 700
Filter 780/60 BP:
PE-Cy7
Laser: 640
Filter 670/30 BP:
APC, Alexa Fluor 647, Alexa Fluor 680, Sytox Red
Filter 730/45 BP:
Alexa Fluor 700, APC-Alexa 700, APC-R700
Filter 780/60 BP:
APC-Cy7, APC-H7
Software Version: BD FACSDiva 8.0
Sample Acquisition: 12mm x 75mm polystyrene tubes, HTS plate reader (96, 384 well plates)
LSRFortessa Cell Analyzer
Optics/Configurations: 5-laser, 18-color
Laser: 355
Filter 450/20 BP:
DAPI, Hoechst Blue, BUV396
Filter 730/45 BP:
BUV737
Laser: 405
Filter 450/50 BP:
Alexa Fluor405, DAPI, Marina Blue, Pacific Blue, eFluor450, Sytox Blue, Calcien Blue, BV421
Filter 525/50 BP:
Alexa Fluor430, AmCyan, CFP, Live Dead fixable Aqua, BV510
Filter 605/12 BP:
Qdot605, Pacific Orange, BV605
Filter 670/30BP:
BV650, Qdot 655
Filter 695/40 BP:
BV711, Qdot 700
Filter 780/60 BP:
BV786, Qdot 800
Laser: 488
Filter 530/30 BP:
Alexa Fluor488, FITC, GFP, Sytox Green, Calcien Green
Filter 710/50 BP:
PerCP, PerCP-Cy5, PerCP-Cy5.5, 7AAD
Laser: 561
Filter 582/15 BP:
PE, DS Red, PI, Alexa Fluor555
Filter 610/20 BP:
PE-Texas Red, Alexa Fluor610, mCherry, mKate, tdTomato, Alexa Fluor594
Filter 660/20 BP:
PE-Cy5
Filter 710/50 BP:
PE-Cy5.5
Filter 780/60 BP:
PE-Cy7
Laser: 640
Filter 670/14 BP:
APC, Alexa Fluor647, Sytox Red
Filter 730/45 BP:
Alexa Fluor700
Filter 780/60 BP:
APC-Cy7, APC-H7
Software Version: BD FACSDiva 8.0.1
Sample Acquisition: 12mm x 75mm polystyrene tubes, HTS Plate Reader (96, 384 well plates)
FACSCanto II Cell Analyzer
Optics/Configurations: 3-laser, 8-color
Laser: 405
Filter 450/50 BP:
Alexa Fluor405, DAPI, Marina Blue, Pacific Blue, eFluor450, Sytox Blue, Calcien Blue, BV421
Filter 510/50 BP:
Alexa Fluor430, AmCyan, CFP, Live Dead fixable Aqua, BV510
Laser: 488
Filter 530/30 BP:
Alexa Fluor488, FITC, GFP, Sytox Green, Calcien Green
Filter 585/42 BP:
PE
Filter 670 LP: (735nm cutoff)
PE-Cy5, PerCP, PerCP-Cy5.5, PI, 7AAD
Filter 780/60 BP:
PE-Cy7
Laser: 633
Filter 660/20 BP:
APC, Alexa Fluor647, Sytox Red
Filter 685 LP:
Alexa Fluor700
Filter 780/60 BP:
APC-Cy7, APC-H7
(Configuration allows only 2 of 3 channels to be selected)
Software Version: BD FACSDiva 8.0.1
Sample Acquisition: 12mm x 75mm polystyrene tubes, HTS plate reader (96 well plates)
Gallios Cell Analyzer
Optics/Configurations: 3-laser, 10-color
Laser: 405
Filter 450/40 BP:
Alexa Fluor405, DAPI, Marina Blue, Pacific Blue, eFluor450, Sytox Blue, Calcien Blue, BV421
Filter 550/40 BP:
Alexa Fluor430, AmCyan, CFP, Live Dead fixable Aqua, BV510, Pacific Orange
Laser: 488
Filter 525/40 BP:
Alexa Fluor488, FITC, GFP, Sytox Green, Calcien Green
Filter 575/30 BP:
PE, DS Red
Filter 620/30 BP:
PE-Texas Red
Filter 695/30 BP:
PE-Cy5.5, PerCP, PerCP-Cy5.5, PI, 7AAD, PE-Cy5
Filter 755 LP:
PE-Cy7
Laser: 638
Filter 660/20 BP:
APC, Alexa Fluor647, Sytox Red
Filter 725/20 BP:
Alexa Fluor700
Filter 755 LP:
APC-Cy7, APC-H7
Software Version: Kaluza for Gallios 1.0.14029.14028
Sample Tube: 12mm x 75mm polystyrene tubes, 32 tube Multi Carousel Loader option
FACSCelesta Cell Analyzer
Optics/Configurations: 3-laser, 12-color
Laser: 405
Filter 450/40 BP:
Alexa Fluor 405, Pacific Blue, eFluor450, Sytox Blue, Calcien Blue, BV421
Filter 525/50 BP:
Alexa Fluor 430, AmCyan, CFP, Live Dead Fixable Aqua, BV510
Filter 610/20 BP:
BV605, Pacific Orange, Qdot 605
Filter 670/30 BP:
BV650, Qdot 655
Filter 710/50 BP:
BV711, Qdot 700
Filter 780/60 BP:
BV786, Qdot 800
Laser: 488
Filter 530/30 BP:
Alexa Fluor 488, FITC, GFP, Sytox Green, Calcien Green
Filter 695/40 BP:
PerCP-Cy5, PerCP-Cy5.5, 7AAD
Laser: 561
Filter 586/15 BP:
PE, DS Red, Alexa Fluor 555
Filter 610/20 BP:
PI, PE-Texas Red, PE-Alexa 610, AlexaFluor 610, PE-CF594, Live Dead Fixable Red, mCherry, mKate, tdTomato, Alexa Fluor 594
Filter 670/30 BP:
PE-Cy5
Filter 780/60 BP:
PE-Cy7
Software Version: BD FACSDiva 8.0.1.1
Sample Acquisition: 12mm x 75mm polystyrene tubes, 96, 384 well plates
ImageStream X Mark II Imaging Cytometer
Optics/Configurations: 5-laser, 12-parameters
Brightfield:
Filter 457/45 BP
Filter 582/25 BP
Laser: 488
Filter 527/65 BP
Alexa Fluor 488, FITC, GFP, Sytox Green, Calcien Green, YFP
Laser: 561
Filter 577/35 BP
PE, DS Red, AlexaFluor 546, PKH26
Filter 610/30 BP
PI, PE-Texas Red, AlexaFluor 610, mCherry, mKate, tdTomato AlexaFluor 568, 7AAD
Filter 702/86 BP
PE-Cy5, PerCP, PerCP-Cy5.5
Filter 762/35 BP
SSC, PE-Cy7, Draq5
Laser: 405
Filter 470/70 BP
Alexa Fluor 405, DAPI, Marina Blue, Pacific Blue, eFluor450, Sytox Blue, Calcien Blue, Live Dead Fixable Aqua
Filter 537/65 BP
Alexa Fluor 430, AmCyan, CFP, Live Dead fixable Aqua, BV510
Laser: 633
Filter 610/30 BP
Qdot 625, eFluor 625
Filter 702/86 BP
APC, Alexa Fluor 647, AlexaFluor 660, Cy5, Draq5, Sytox Red
Filter 762/35 BP
SSC, APC-Cy7, APC-H7, Cy7, AlexaFluor 750
Software Version: INSPIRE IDEAS 6.2.64
Sample Acquisition: 1.5ml microcentrifuge tubes, 96-well plates
Magnification Power:
20x – 1.0uM/Pixel; 4,000 cells/second
40x – 0.5uM/Pixel; 2,000 cells/second
60x – 0.3uM/Pixel; 1,200 cells/second
DMi8 Microscope
Optics/Configurations: 4-color, 4-objective
Illuminated Lightpath:
Brightfield
Phase
Polarized
Fluorescent Colors (Ex/Em):
DAPI: 325-375/435-485
FITC: 460-500/512-542
TxR: 540-580/592-668
Y5: 590-650/662-738
Capture Features:
Time lapse
Z-stack
Large field capture/stitching
Objectives:
5x
10x
20x
40x
Added Magnification Changer:
1.0x
1.5x
2.0x
Software Version: Leica Application Suite X 1.1.0.12420
Imaging Devices:
Slides
Dishes
Flasks
6-, 24-, 96-, 384-well plates
Cell Sorting Cytometers
- FACSAria Fusion - More Info
- Influx - More Info
- FACSAria IIIu #1 - More Info
- FACSAria IIIu #2 - More Info
- FACSJazz - More Info
Analytical Flow Cytometers
- LSRFortessa X-20 - More Info
- LSRFortessa X-20 Left - More Info
- LSRFortessa - More Info
- FACSCanto II - More Info
- Gallios - More Info
- FACSCelesta - More Info