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South Campus Instrumentation

FACSAria Fusion Cell Sorter*

Optics/Configurations: 6-laser, 15-color

Laser: 407

    Filter 450/50 BP:
        Alexa Fluor405, DAPI, Marina Blue, Pacific Blue, eFluor450, Sytox Blue, Calcien Blue, BV421
    Filter 525/50 BP:
        Alexa Fluor430, AmCyan, CFP, Live Dead fixable Aqua, BV510
    Filter 610/20 BP:
        BV605, Pacific Orange, Qdot 605
    Filter 660/20 BP:
        BV650, Qdot 655
    Filter 710/50 BP:
        BV711, Qdot 700
    Filter 780/60 BP:
        BV786, Qdot 800

Laser: 488

    Filter 530/30 BP:
        Alexa Fluor488, FITC, GFP, Sytox Green, Calcien Green
    Filter 695/40 BP:
        PE-Cy5, PerCP, PerCP-Cy5, PerCP-Cy5.5, PI, 7AAD

Laser: 561

    Filter 582/15 BP:
        PE, DS Red
    Filter 610/20 BP:
        PE-Texas Red, Alexa Fluor610, mCherry, mKate, tdTomato
    Filter 670/14 BP:
        PE-Cy5
    Filter 780/60 BP:
        PE-Cy7

Laser: 633

    Filter 670/30 BP:
        APC, Alexa Fluor647, Sytox Red
    Filter 730/45 BP:
        Alexa Fluor700
    Filter 780/60 BP:
    APC-Cy7, APC-H7

Lasers 375 and 445 additionally available. Contact flow staff for more information.

Software Version: BD FACSDiva 8.0

Sample Tube: 12mm x 75mm tube

Sample Collection: 15ml tubes (up to two populations), 12mm x 75mm tubes (up to four populations), 1.5ml microcentrifuge tubes (up to four populations), 24, 96, 384 well plates

Nozzle/Pressure: 70um/70psi (default), 100um/30psi (by request; call flow staff for more information)
*Class II Biosafety Cabinet

Influx Silver Cell Sorter

Optics/Configurations: 5-laser, 14-color

Laser: 355

    Filter 460/50 BP:
        Hoechst Blue, DAPI
    Filter 670/30 BP:
        Hoechst Red

Laser: 405

    Filter 460/50 BP:
        Alexa Fluor405, Marina Blue, Pacific Blue, eFluor450, Sytox Blue, Calcien Blue, BV421
    Filter 520/35 BP:
        Alexa Fluor430, AmCyan, CFP, Live Dead fixable Aqua, BV510

Laser: 488

    Filter 530/40 BP:
        Alexa Fluor488, FITC, GFP, Sytox Green, Calcien Green
    Filter 670/30 BP:
        PerCP, PI, 7AAD
    Filter 710/50 BP:
        PerCP-Cy5, PerCP-Cy5.5

Laser: 561

    Filter 585/29 BP:
        PE, DS Red
    Filter 610/20 BP:
        PE-Texas Red, Alexa Fluor610, mCherry, mKate, tdTomato
    Filter 670/30 BP:
        PE-Cy5
    Filter 750 LP:
        PE-Cy7

Laser: 642

    Filter 670/30 BP:
        APC, Alexa Fluor647, Sytox Red
    Filter 720/40 BP:
        Alexa Fluor700
    Filter 750 LP:
        APC-Cy7, APC-H7

Software Version: BD Sortware 1.2.0.142

Sample Tube: 12mm x 75mm polypropylene tube

Sample Collection: 15ml tubes (up to two populations), 12mm x 75mm tubes (up to four populations), 50ml tube (up to two populations), 1.5ml microcentrifuge tubes (up to six populations), 24, 96, 384 well plates

Nozzle/Pressure: 100um/27 psi (default), 70um/52psi (by request; call flow staff for more information)

Influx Green Cell Sorter

Optics/Configurations: 5-laser, 21-color

Laser: 355

    Filter 379/34 BP:
        BUV395
    Filter 460/50 BP:
        DAPI, BUV496, Live Dead 450-UV, Hoechst 33342, CF350, AlexaFluor 350
    Filter 670/30 BP:
        BUV661, Hoechst Red
    Filter 715 LP:
        BUV737, BUV805

Laser: 405

    Filter 440/40 BP:
        BFP, BV421, Sytox Blue, AlexaFluor 405, eFluor450, SuperBright 436
    Filter 480/40 BP:
        CFP, BV510, BV480, eFluor506
    Filter 610/20 BP:
        BV605, SuperBright600
    Filter 660/20 BP:
        BV650, SuperBright645
    Filter 710/50 BP:
        BV711, SuperBright702
    Filter 747/33 BP:
        BV750
    Filter 780/60 BP:
        BV785, BV786, SuperBright780

Laser: 488

    Filter 530/40 BP:
        FITC, GFP, YFP, AlexaFluor 488, Sytox Green, Calcien, BB515, NovaBlue530, SparkBlue-550
    Filter 650 LP:
        PerCP-Cy5.5, PerCP, 7AAD, BB700, BB790, PerCP-eFluor710, NovaBlue660

Laser: 561

    Filter 585/29 BP:
        PE, DS Red, AlexaFluor 555, BYG584, eFluor570, NovaYellow570, SparkYG-570
    Filter 610/20 BP:
        PE-CF594, PE-Dazzle594, eFluor615, RFP (mCherry, mKate, TdTomato, Turbo), AlexaFluor 594, NovaYellow610, PI, PE-TexasRed
    Filter 670/30 BP:
        PE-Cy5, BYG660, PE-Fire640, NovaYellow660
    Filter 710/50 BP:
        PE-Cy5.5, PE-Fire700
    Filter 780/60 BP:
        PE-Cy7, BYG790

Laser: 642

    Filter 670/30 BP:
        APC, AlexaFluor 647, Sytox Red, eFluor660, SparkNIR-685, Draq5
    Filter 720/40 BP:
        AlexaFluor 700
    Filter 780/60 BP:
        APC-Cy7, APC-H7, APC-eFluor780, APC-Fire750, APC-Fire810

Software Version: BD SortWare 1.2.0.142

Sample Tube: 12mm x 75mm polypropylene tube

Sample Collection: 15ml tubes (up to two populations),12mm x 75mm tubes (up to six populations), 50ml tube (up to two populations), 1.5ml microcentrifuge tubes (up to six populations), 24, 96, 384 well plates

Nozzle/Pressure: 70um/52psi, 100um/27psi

FACSAria IIIu #1 Cell Sorter

Optics/Configurations: 4-laser, 14-color

Laser: 405

    Filter 450/50 BP:
        Alexa Fluor 405, DAPI, Marina Blue, Pacific Blue, eFluor450, Sytox Blue, Calcien Blue, BV421
    Filter 530/30 BP:
        Alexa Fluor 430, AmCyan, CFP, Live Dead fixable Aqua, BV510
    Filter 610/20 BP:
        BV605, Pacific Orange, Qdot 605
    Filter 670/30 BP:
        BV650, Qdot 655
    Filter 710/40 BP:
        BV711, Qdot 700
(Filter 780/60 BP for BV786 Available Upon Request)

Laser: 488

    Filter 530/30 BP:
        Alexa Fluor 488, FITC, GFP, Sytox Green, Calcien Green
    Filter 695/40 BP:
        PerCP, PerCP-Cy5, PerCP-Cy5.5, 7AAD

Laser: 561

    Filter 582/15 BP:
        PE, DS Red
    Filter 610/20 BP:
        PI, PE-Texas Red, Alexa Fluor 610, mCherry, mKate, tdTomato
    Filter 670/40 BP:
        PE-Cy5
    Filter 780/60 BP:
        PE-Cy7

Laser: 633

    Filter 660/20 BP:
        APC, Alexa Fluor 647, Sytox Red
    Filter 720/40 BP:
        Alexa Fluor 700
    Filter 780/60 BP:
        Alexa Fluor 700, APC-Cy7, APC-H7

Software Version: BD FACSDiva 8.0.1

Sample Tube: 12mm x 75mm tube

Sample Collection: 15ml tubes (up to two populations), 12mm x 75mm tubes (up to four populations), 1.5ml microcentrifuge tubes (up to four populations), 24, 96, 384 well plates

Nozzle/Pressure: 70um/70psi (default), 100um/30psi (by request; call flow staff for more information)

FACSAria IIIu #2 Cell Sorter

Optics/Configurations: 4-laser, 16-color

Laser: 405

    Filter 450/40 BP:
        Alexa Fluor 405, DAPI, Marina Blue, Pacific Blue, eFluor450, Sytox Blue, Calcien Blue, BV421
    Filter 530/30 BP:
        Alexa Fluor 430, AmCyan, CFP, Live Dead Fixable Aqua, BV510
    Filter 575/25 BP:
        Pacific Orange, BV570
    Filter 610/20 BP:
        BV605, Qdot 605
    Filter 670/30 BP:
        BV650, Qdot 655
    Filter 710/40 BP:
        BV711, Qdot 700
    Filter 780/60 BP:
        BV786

Laser: 488

    Filter 530/30 BP:
        Alexa Fluor488, FITC, GFP, Sytox Green, Calcien Green
    Filter 695/40 BP:
        PerCP, PerCP-Cy5, PerCP-Cy5.5, 7AAD

Laser: 561

    Filter 582/15 BP:
        PE, DS Red
    Filter 610/20 BP:
        PI, PE-Texas Red, AlexaFluor 610, mCherry, mKate, tdTomato
    Filter 670/40 BP:
        PE-Cy5
    Filter 780/60 BP:
        PE-Cy7

Laser: 640

    Filter 660/20 BP:
        APC, Alexa Fluor647, Sytox Red
    Filter 780/60 BP:
        Alexa Fluor700, APC-Cy7, APC-H7

Software Version: BD FACSDiva 8.0.1

Sample Tube: 12mm x 75mm tube

Sample Collection: 15ml tubes (up to two populations),12mm x 75mm tubes (up to four populations),1.5ml microcentrifuge tubes (up to four populations)

Nozzle/Pressure: 70um/70psi (default), 100um/30psi (by request; call flow staff for more information)

LSRFortessa X-20 Cell Analyzer (Right)

Optics/Configurations: 5-laser, 18-color

Laser: 355

Filter 379/28 BP:
Hoechst Blue, BUV396
Filter 740/35 BP:
BUV737

Laser: 405

Filter 450/50 BP:
    Alexa Fluor 405, Marina Blue, Pacific Blue, eFluor450, Sytox Blue, Calcien Violet, BV421, DAPI
Filter 525/50 BP:
    Alexa Fluor 430, AmCyan, CFP, Live/Dead fixable Aqua, BV510
Filter 610/20 BP:
    BV605, Pacific Orange, Qdot 605
Filter 670/30 BP:
    BV650, Qdot 655
Filter 710/50 BP:
    BV711, Qdot 700
Filter 780/60 BP:
    BV786, Qdot 800

Laser: 488

Filter 530/30 BP:
Alexa Fluor 488, FITC, GFP, Sytox Green, Calcien Green
Filter 710/50 BP:
PerCP, PerCP-Cy5, PerCP-Cy5.5, 7-AAD

Laser: 561

Filter 586/15 BP:
    PE, DS Red, PI
Filter 610/20 BP:
    PE-Texas Red, PE-Alexa 610, PE-CF594, Live/Dead Fixable Red, Alexa Fluor 610, mCherry, mKate, tdTomato
Filter 670/30 BP:
    PE-Cy5
Filter 710/50 BP:
    PE-Cy5.5, PE-Alexa 700
Filter 780/60 BP:
    PE-Cy7

Laser: 640

Filter 670/30 BP:
    APC, Alexa Fluor 647, Alexa Fluor 680, Sytox Red
Filter 730/45 BP:
    Alexa Fluor 700, APC-Alexa 700, APC-R700
Filter 780/60 BP:
    APC-Cy7, APC-H7

Software Version: BD FACSDiva 8.0

Sample Acquisition: 12mm x 75mm polystyrene tubes, HTS plate reader (96, 384 well plates)

LSRFortessa X-20 Cell Analyzer (Left)

Optics/Configurations: 5-laser, 18-color

Laser: 355

Filter 379/28 BP:
Hoechst Blue, BUV396
Filter 740/35 BP:
BUV737

Laser: 405

Laser: 488

Filter 530/30 BP:
Alexa Fluor 488, FITC, GFP, Sytox Green, Calcien Green
Filter 710/50 BP:
PerCP, PerCP-Cy5, PerCP-Cy5.5, 7-AAD

Laser: 561

Laser: 640

Filter 670/30 BP:
    APC, Alexa Fluor 647, Alexa Fluor 680, Sytox Red
Filter 730/45 BP:
    Alexa Fluor 700, APC-Alexa 700, APC-R700
Filter 780/60 BP:
    APC-Cy7, APC-H7

Software Version: BD FACSDiva 8.0

Sample Acquisition: 12mm x 75mm polystyrene tubes, HTS plate reader (96, 384 well plates)

LSRFortessa Cell Analyzer

Optics/Configurations: 5-laser, 18-color

Laser: 355

Filter 450/20 BP:
DAPI, Hoechst Blue, BUV396
Filter 730/45 BP:
BUV737

Laser: 405

Filter 450/50 BP:
    Alexa Fluor405, DAPI, Marina Blue, Pacific Blue, eFluor450, Sytox Blue, Calcien Blue, BV421
Filter 525/50 BP:
    Alexa Fluor430, AmCyan, CFP, Live Dead fixable Aqua, BV510
Filter 605/12 BP:
    Qdot605, Pacific Orange, BV605
Filter 670/30BP:
    BV650, Qdot 655
Filter 695/40 BP:
    BV711, Qdot 700
Filter 780/60 BP:
    BV786, Qdot 800

Laser: 488

Filter 530/30 BP:
Alexa Fluor488, FITC, GFP, Sytox Green, Calcien Green
Filter 710/50 BP:
PerCP, PerCP-Cy5, PerCP-Cy5.5, 7AAD

Laser: 561

Filter 582/15 BP:
    PE, DS Red, PI, Alexa Fluor555
Filter 610/20 BP:
    PE-Texas Red, Alexa Fluor610, mCherry, mKate, tdTomato, Alexa Fluor594
Filter 660/20 BP:
    PE-Cy5
Filter 710/50 BP:
    PE-Cy5.5
Filter 780/60 BP:
    PE-Cy7

Laser: 640

Filter 670/14 BP:
    APC, Alexa Fluor647, Sytox Red
Filter 730/45 BP:
    Alexa Fluor700
Filter 780/60 BP:
    APC-Cy7, APC-H7

Software Version: BD FACSDiva 8.0.1

Sample Acquisition: 12mm x 75mm polystyrene tubes, HTS Plate Reader (96, 384 well plates)

Cytek Aurora Analyzer

Optics/Configurations: 5-laser, 40-color

Laser: 355

Laser: 405

Laser: 488

Laser: 561

Laser: 640

Software: SpectroFlo

Sample Acquisition: 12mm x 75mm polystyrene tubes, 96 well plates

FACSCanto II Cell Analyzer

Optics/Configurations: 3-laser, 8-color

Laser: 405

Filter 450/50 BP:
Alexa Fluor405, DAPI, Marina Blue, Pacific Blue, eFluor450, Sytox Blue, Calcien Blue, BV421
Filter 510/50 BP:
Alexa Fluor430, AmCyan, CFP, Live Dead fixable Aqua, BV510

Laser: 488

Filter 530/30 BP:
    Alexa Fluor488, FITC, GFP, Sytox Green, Calcien Green
Filter 585/42 BP:
    PE
Filter 670 LP: (735nm cutoff)
    PE-Cy5, PerCP, PerCP-Cy5.5, PI, 7AAD
Filter 780/60 BP:
    PE-Cy7

Laser: 633

Filter 660/20 BP:
    APC, Alexa Fluor647, Sytox Red
Filter 685 LP:
    Alexa Fluor700
Filter 780/60 BP:
    APC-Cy7, APC-H7
(Configuration allows only 2 of 3 channels to be selected)

Software Version: BD FACSDiva 8.0.1

Sample Acquisition: 12mm x 75mm polystyrene tubes, HTS plate reader (96 well plates)

Gallios Cell Analyzer

Optics/Configurations: 3-laser, 10-color

Laser: 405

Filter 450/40 BP:
Alexa Fluor405, DAPI, Marina Blue, Pacific Blue, eFluor450, Sytox Blue, Calcien Blue, BV421
Filter 550/40 BP:
Alexa Fluor430, AmCyan, CFP, Live Dead fixable Aqua, BV510, Pacific Orange

Laser: 488

Filter 525/40 BP:
    Alexa Fluor488, FITC, GFP, Sytox Green, Calcien Green
Filter 575/30 BP:
    PE, DS Red
Filter 620/30 BP:
    PE-Texas Red
Filter 695/30 BP:
    PE-Cy5.5, PerCP, PerCP-Cy5.5, PI, 7AAD, PE-Cy5
Filter 755 LP:
    PE-Cy7

Laser: 638

Filter 660/20 BP:
    APC, Alexa Fluor647, Sytox Red
Filter 725/20 BP:
    Alexa Fluor700
Filter 755 LP:
    APC-Cy7, APC-H7

Software Version: Kaluza for Gallios 1.0.14029.14028

Sample Tube: 12mm x 75mm polystyrene tubes, 32 tube Multi Carousel Loader option

FACSCelesta Cell Analyzer

Optics/Configurations: 3-laser, 12-color

Laser: 405

    Filter 450/40 BP:
        Alexa Fluor 405, Pacific Blue, eFluor450, Sytox Blue, Calcien Blue, BV421
    Filter 525/50 BP:
        Alexa Fluor 430, AmCyan, CFP, Live Dead Fixable Aqua, BV510
    Filter 610/20 BP:
        BV605, Pacific Orange, Qdot 605
    Filter 670/30 BP:
        BV650, Qdot 655
    Filter 710/50 BP:
        BV711, Qdot 700
    Filter 780/60 BP:
        BV786, Qdot 800

Laser: 488

Filter 530/30 BP:
Alexa Fluor 488, FITC, GFP, Sytox Green, Calcien Green
Filter 695/40 BP:
PerCP-Cy5, PerCP-Cy5.5, 7AAD

Laser: 561

    Filter 586/15 BP:
        PE, DS Red, Alexa Fluor 555
    Filter 610/20 BP:
        PI, PE-Texas Red, PE-Alexa 610, AlexaFluor 610, PE-CF594, Live Dead Fixable Red, mCherry, mKate, tdTomato, Alexa Fluor 594
    Filter 670/30 BP:
        PE-Cy5
    Filter 780/60 BP:
        PE-Cy7

Software Version: BD FACSDiva 8.0.1.1

Sample Acquisition: 12mm x 75mm polystyrene tubes, 96, 384 well plates

ImageStream X Mark II Imaging Cytometer

Optics/Configurations: 5-laser, 12-parameters

Brightfield:

Filter 457/45 BP
Filter 582/25 BP

Laser: 488

Filter 527/65 BP
Alexa Fluor 488, FITC, GFP, Sytox Green, Calcien Green, YFP

Laser: 561

    Filter 577/35 BP
        PE, DS Red, AlexaFluor 546, PKH26
    Filter 610/30 BP
        PI, PE-Texas Red, AlexaFluor 610, mCherry, mKate, tdTomato AlexaFluor 568, 7AAD
    Filter 702/86 BP
        PE-Cy5, PerCP, PerCP-Cy5.5
    Filter 762/35 BP
        SSC, PE-Cy7, Draq5

Laser: 405

    Filter 470/70 BP
        Alexa Fluor 405, DAPI, Marina Blue, Pacific Blue, eFluor450, Sytox Blue, Calcien Blue, Live Dead Fixable Aqua
    Filter 537/65 BP
        Alexa Fluor 430, AmCyan, CFP, Live Dead fixable Aqua, BV510

Laser: 633

    Filter 610/30 BP
        Qdot 625, eFluor 625
    Filter 702/86 BP
        APC, Alexa Fluor 647, AlexaFluor 660, Cy5, Draq5, Sytox Red
    Filter 762/35 BP
        SSC, APC-Cy7, APC-H7, Cy7, AlexaFluor 750

Software Version: INSPIRE IDEAS 6.2.64

Sample Acquisition: 1.5ml microcentrifuge tubes, 96-well plates

Magnification Power:

    20x – 1.0uM/Pixel; 4,000 cells/second
    40x – 0.5uM/Pixel; 2,000 cells/second
    60x – 0.3uM/Pixel; 1,200 cells/second

DMi8 Microscope

Optics/Configurations: 4-color, 4-objective

Illuminated Lightpath:
    Brightfield
    Phase
    Polarized

Fluorescent Colors (Ex/Em):
    DAPI:   325-375/435-485
    FITC:    460-500/512-542
    TxR:     540-580/592-668
    Y5:       590-650/662-738

Capture Features:
    Time lapse
    Z-stack
    Large field capture/stitching

Objectives:
    5x
    10x
    20x
    40x

Added Magnification Changer:
    1.0x
    1.5x
    2.0x

Software Version: Leica Application Suite X 1.1.0.12420

Imaging Devices:
    Slides
    Dishes
    Flasks
    6-, 24-, 96-, 384-well plates

South Campus Instrumentation

Cell Sorting Cytometers

Analytical Flow Cytometers

Imaging Cytometers

Microscope

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