The BSF core has a discount arrangement with Genscript for all cloning and mutagenesis requests as well as a set of customized vectors that have N-terminal or C-terminal tags with TEV protease cleavage sites for tag removal during the purification, as well as access to the suite of vectors that Genscript have available:
N-terminal fusion vectors: His6-TEV, His6-MBP-TEV, His6-Sumo-TEV, His6-GST-TEV, His6-Strep2-TEV
C-terminal fusion vectors: His6
We pass on the discounted cloning and mutagenesis price to you as the customer without any additional administration charge.
Premium Expression Testing
Matrix of 16 conditions tested for expression:
- 4x E. coli strains: BL21(DE3), Rosetta pLysS, C41 and SoluBL21
- 4x growth media: Auto-induction ZYP5052, Superior, Power and Terrific broths
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Budget Expression Testing
E. coli Rosetta2 pLysS in auto-induction media
- Not available for requests with <13 plasmids
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The protein purification service is split into two stages:
- 2L scale test purification is used to identify the optimal purification method and assess protein yield.
- Large scale prep: 6L, 12L options available.
You will receive a certificate of analysis for the protein with SDS-PAGE gel analysis of the protein purity. Additional quality control experiments, Mass spectrometry and Western Blot analysis are available on request for an additional fee.
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