With the help from our donors, CROR has established the Chemical Imaging Research Core at MD Anderson. The core consists of four brand new technologies to the MD Anderson community that will allow our scientists to continue pursuing cutting edge studies. The shared resource is led by Erik Cressman, M.D., Ph.D.(Director) and Dodge Baluya, Ph.D. (Manager). If interested in using any of the available instruments, please contact:
Dodge L. Baluya, Ph.D.
Department of Diagnostic Imaging-Interventional Radiology
Scientific Manager, CROR Instrumentation Core
Center for Radiation Oncology Research
Agilent 7900 ICP-MS
ICP-MS is primarily used for quantitative elemental analysis with high sensitivity (11 orders of magnitude). Samples are dissolved and introduced as a spray into a plasma cone, and thus subsequently atomized and ionized. The resulting species are detected according to their masses (mass-to-charge ratios), which allows it to be identified and quantified. Common applications for this mass spectrometry technique is in the medical and toxicological fields, where one could be interested in quantitative metal assays from any biological fluids. It is also heavily used in environmental analysis for soil and water testing.
Cytoviva Hyperspectral Microscope
CytoViva’s Hyperspectral Imaging technology was specifically designed to provide quantitative spectral analysis of nanoscale materials imaged with the patented darkfield-based microscope system or with other microscopy modalities. This can include spectral analysis of both biological and materials-based nanoscale samples, which may be isolated or integrated in cells, tissue or other materials-based matrices. Using the proprietary image analysis software, it is possible to identify, locate and map nano-scale materials within a sample based on the unique spectral response of this material. This can be accomplished without any special staining or tagging of the target material, as is often required with traditional microscopy techniques. In addition, when functional groups (tagging) are added onto or into a nanoscale sample, they can often be identified, as this change in the sample’s surface chemistry will be spectrally detected.
Waters Synapt G2-Si with MALDI, DESI, iKnife
This instrument is a high resolution mass spectrometer specifically configured for imaging biological and chemical materials. Imaging of samples (e.g. tissue mounted on microscope slides) is achieved using a solid state laser (Matrix-Assisted Laser Desorption Ionization, MALDI) or a spray (Desorption Electrospray Ionization, DESI) rasterized across the tissue sample, giving a chemical composition profile on each corresponding spatial coordinate. These mass spectral information are collated by the software to produce a chemical image that can be correlated to the sample histological profile. Examples of applications of mass spectrometry imaging are profiling phospholipids in rat brain and determination of drug spatial distribution in various tissue types.
Shimadzu IM Layer (for MALDI Mass Spectrometry Imaging)
To assist in sample preparation for MALDI mass spectrometry imaging, the Shimadzu IM Layer is used to achieve consistent and uniform application of chemical matrix (needed to enhance detection by assisting the ionization process). Having a homogenous layer will minimize introduction of residual artifacts that will affect the resulting chemical image. The application procedure used is vapor deposition method, which is computer-controlled and user-friendly.