Services

SAMPLE SUBMISSION AND PAYMENT

How to Submit Samples for Processing

    1. For Proteomics: Email David Hawke at dhawke@mdanderson.org to set an appointment to discuss the details of your experimental design.

        For Metabolomics: Email Phil Lorenzi at PLLorenzi@mdanderson.org to set an appointment to discuss the details of your experimental design.

    2. Go to mdanderson.ilabsolutions.com to register your online account. Once that is completed, you can begin to request services. For more detailed instructions on setting up your account, please Click Here

    3. Upon approval of your online service request, you will be notified via email to set an appointment for sample drop off. **Note: Samples will not be accepted without an appointment!

Payment Methods

INSIDE MD ANDERSON:

  • PeopleSoft Account Number

OUTSIDE MD ANDERSON:

  • Credit Card or Purchase Order (PO)

**ALL OUTSIDE INSTITUTIONS ARE SUBJECT TO 60% OVERHEAD FEE**

 

Other Forms

Credit Card Authorization Form (pdf) 

Bio-Specimen Transportation Form (MD Anderson Employees Only) (pdf)

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SERVICES WE PROVIDE:

Proteomics

Protein Identification

  •  High sensitivity (silver stain is OK), up to 100 proteins or more may be identifiable.
  •  Cost: $200/sample (+60% Overhead for Outside Institutions)
  •  Turnaround Time: 5-10 working days

Molecular Weight Determination

  •  Check the MW of your peptide or molecule. Proteins may also be measured if enough clean protein is available  (larger proteins are less reliable)
  •  Cost: $50/sample (+60% Overhead for Outside Institutions)
  •  Turnaround Time: 4-10 working days

Quantitative Protein Analysis

  • Using stable isotope methods, iTRAQ, etc.
  • Using non-isotopic methods.
    • Please Inquire
    • For SRM/MRM methods on our tandem quadrupole MS please inquire.

Posttranslational Modification Analysis

  •  Phosphorylation and acetylation. Please inquire for more detailed information and other analyses that can be performed. 
  •  Cost: $500/sample (+60% Overhead for Outside Institutions)
  •  Turnaround Time: Will vary

LC-MS Analysis

  •  Size exclusion chromatography and simple LC-MS. Please inquire for more detailed information and other analyses that can be performed.
  •  Cost: $100/sample (+60% Overhead for Outside Institutions)
  •  Turnaround Time: Will vary

Large-Scale PhosphoProteomics

  •  Please Inquire

Stable-Isotope Guided Protein Profiling (Tumor-associated antigens, metabolic enzymes, etc)

  •  Please Inquire

Do you need some other MS service?

  •  Please Inquire - Consultations for custom assays for other MS experiments are also available. 

 

Metabolomics

New Assay Setup

    • Develop assay for a new metabolite/analyte
    • Cost: $1000/analyte (+60% Overhead for Outside Institutions)-Pricing may vary for this service

Sample Preparation Assistance

    • Onsite assistance with sample preparation
    • Cost: $150/sample (+60% Overhead for Outside Institutions)

Amino Acids (30)

    • Quantitative analysis of 18 proteogenic AAs + 12 related metabolites
    • Cost: $150/sample (+60% Overhead for Outside Institutions)

Organic Acids (8)

    • Quantitative analysis of 8 organic acids
    • Cost: $150/sample (+60% Overhead for Outside Institutions)

Glutathine (2)

    • Quantitative analysis of GSH and GSSG
    • Cost: $150/sample (+60% Overhead for Outside Institutions)

 

 

Please email (preferred) or call for an appointment to discuss your project!

   

Silver Staining of Protein Gels

Recommended Silver Stain

Download Silver Stain Protocol (pdf).  Please ignore destain, we destain after cutting the bands

Reference:  Morrissey, J.H. (1981).Silver stain for proteins in polyacrylamide gels: A modified procedure with enhanced uniform sensitivity. Anal. Biochem. 117: 307-310.

Reagents

Silver Solution

  • 0.1% AgNO3 in H2O: 0.1g AgNO3 dissolved in 100 ml H2O

Developing Solution

  • 3% Sodium carbonate (Na2CO3) in H2O
  • 50 µl formaldehyde/100 ml
  • Stir for 2 minutes

Procedure

All incubations are at room temperature, and carried out with gentle shaking on a shaking platform. All steps take place in a clean (acid washed) pyrex dish. 

Make up all solutions fresh, just before use.

    • Incubate 15 min in 50% methanol
    • Incubate 15 min in 32 µM DTT (8 µl 1M DTT /250 ml water) solution (freshly made)
    • Wash briefly (approximately 5 to 10 seconds) with H2O, twice
    • Wash with a little Silver Solution, pour away, then add the rest of the solution and incubate for 15 minutes
    • Wash briefly (approximately 5 to 10 seconds) with H2O, twice
    • Twice wash with a little Developing Solution, pour away, then add the rest of the solution and incubate until the bands are the desired intensity
    • Pour off most of the developing solution, sprinkle solid citric acid into the solution containing the gel and swirl. Continue slowly adding the citric acid until the fizzing ceases
    • Add a little H2O, and incubate 15 minutes
    • Incubate 3 X 15 minutes with 3 changes of H2O
    • Preferred: Bring the gel to our lab; we prefer to cut the bands here

     

    Results from Proteomics Tracker

    Please click on https://biostatistics.mdanderson.org/ProteomicsTracker  

     

    **We gratefully acknowledge the NIH High-End Instrumentation program grant# 1S10OD012304-01 for funding the acquisition of our new Orbitrap Fusion**