Proteomics and Metabolomics Facility
OUR FACILITY IS CURRENTLY USING ILAB SOLUTIONS FOR ALL SERVICE REQUESTS.
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The Proteomics Facility at The University of Texas MD Anderson Cancer Center was founded in 2002. Metabolomics was added in 2014.
The facility provides state-of-the-art mass spectrometry analysis of proteins and metabolites for both basic and clinical cancer research. We work with internal as well as external researchers.
Please remember to acknowledge your use of the Facility in your publications, and keep us informed when you do so! Also, please thank the M.D. Anderson Cancer Center, NIH High-End Instrumentation program grant# 1S10OD012304-01, and CPRIT Core Facility Grant# RP130397 for supporting us.
The primary mission of the Proteomics and Metabolomics Facility is to serve the faculty of the MD Anderson Cancer Center by providing access to mass-spectrometry based proteomics and metabolomics technologies and services.
What is the Proteome?
The term proteome was coined in the mid-1990s to describe the set of proteins encoded by the genome.
-ome: Transcriptome, interactome, degradome, metabolome, secretome, etc.
What is Proteomics?
Proteomics is the study of proteomes or the systematic analysis of proteins.
It is pronounced as [pro•te•o•mics] (prOt-E-'O-miks) but not [proteonomics].
As defined by Merriam-Webster dictionary: a branch of biotechnology concerned with applying the techniques of molecular biology, biochemistry and genetics to analyzing the structure, function and interactions of the proteins produced by the genes of a particular cell, tissue or organism, with organizing the information in databases, and with applications of the data (as in medicine or biology).
Proteomics was originally used for the large scale identification of proteins separated by 2D gel electrophoresis. However, proteomics now includes not only the analysis of all expressed protein analysis but also traditional protein structural studies such as protein identification, post-translational modification, protein-protein interactions and other functional interests related with protein structure.
What is Metabolomics?
Metabolomics is the study of small molecules (defined generally by molecular weight less than 1500 Daltons). “Targeted Metabolomics” refers to the analysis of pre-defined molecules, whereas “Non-Targeted Metabolomics” refers to the analysis of all detectable small molecules.
How Can We Help You?
Please see Services for more information!
Our primary service is Protein Identification. Proteins are digested with protease and analyzed by mass spectrometry. In a similar way, we can also attempt to find and localize post-translational modifications. For some experiments a simple molecular weight determination may be sufficient, please call 713-834-6096 or e-mail us at firstname.lastname@example.org to discuss the specific needs of your project. If your project requires other proteomic or metabolomic analyses we can also help you connect with the appropriate resources.
1. Label-Free Quantitation. emPAI, peak-counting, and area-under-the-curve methods are all available at no additional charge.
2. Mass-Westerns. Please enquire about how this targeted proteomics technology can help you.
3. Protein MW determination. Sometimes intact protein analysis by LCMS can reveal modifications.
4. Peptide QC. Check peptide identity and quality. MW only, or MW plus MS/MS to confirm sequence.
5. Quantitative Proteomics. We can now offer "Stable-Isotope Guided Protein Profiling" using reference libraries of SIL-peptides, which can also be used in quantitative SRM/MRM experiments. Lists include: the ABRF 1000 peptide list (JPT), 75-protein Tumor Associated Antigens (JPT), 24-protein Metabolic enzymes (JPT), and 76-Biomarkers (MRM-Proteomics). Others are possible, please inquire for details.
1. Amino Acids (30): Quantitative LC-MS assay for measurement of the 18 proteogenic amino acids and 12 related molecules.
2. Onsite experiments with coaching and assistance by our staff.
3. Proteomics/Metabolomics. Live-cell imaging and on-stage incubation with EVOS FL-Auto microscopy system – please inquire.