BRAF Mutational Analysis


To identify activating point mutations in the kinase domain of BRAF, an oncogene that is frequently mutated in many human malignancies including melanoma, colorectal cancer (CRC), papillary thyroid cancer, and lung cancer. BRAF mutations are found in sporadic microsatellite instability-high (MSI-H) CRC but not in CRC arising from HNPCC and therefore in concert with the hMLH1 methylation assay may be useful in determining germline versus sporadic MSI-H CRC. In addition, point mutations in BRAF may identify patients likely to benefit from treatment by BRAF inhibitors.


Next Gen Sequencing of codons 444, 464, 466, 469, 471, 581, 586, 587, 592, 594-597, 599-602, 601 and 605 is performed on tumor cells extracted from paraffin embedded tissue.  This assay is also available as PCR-based pyrosequencing of DNA to examine codons 595 to 600 from exon 15 (the most common mutation site) and codons 468 to 474 from exon 11 of the BRAF gene.


Tumor clone must comprise at least 10% of the cells in the sample.

Turnaround Time

10 days

Sample Requirements

  • 10ug of purified DNA, sent on dry ice


  • Four to six unstained recut slides of formalin-fixed, paraffin embedded tissue containing adequate amounts of tumor to be analyzed (See Sensitivity).  The area of tumor to be analyzed should be indicated by circling the area on the bottom side of the slide or in a separate H&E-stained guide section.

    Please provide a copy of the corresponding pathology report.

CPT Codes


Additional charges may apply for tissue extraction.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.