shRNA and ORFeome Core

shRNA and ORFeome Core

The discovery of short hairpin RNAs (shRNAs) suppressing gene expression in mammalian cells enables large-scale loss-of-function screens by using genome-wide shRNA libraries. Genome scale over-expression libraries allow large-scale gain-of-function screens. The ShRNA and ORFeome core makes available to the MD Anderson research community the human and mouse shRNA libraries, human ORFeome collaboration and LentiORF libraries ( The core facility can provide individual shRNA plasmids and cDNA clones for MDACC laboratories, or carry out a screening experiment using the libraries. Purchase of clones or libraries through the shRNA and ORFeome core reduces costs and decreases turnaround time for researchers.


The UT MD Anderson Cancer Center
Department of Molecular and Cellular Oncology
1515 Holcombe Blvd, Room Y8.5719, Houston, TX 77030

Available Libraries

Human and mouse ShRNA libraries: GIPZ Lentiviral shRNAmir libraries targets the entire human and mouse genome with multiple shRNAmir per target gene.

Human ORFeome Collaboration: each clone contains a sequence-verified open reading frame (ORF) subcloned into Gateway™ recombinational entry vector.

Human Precision LentiORFs: an expression-ready library, lentiviral ORFs.

Pre-Assembled Sub-libraries:

  • Human Kinase shRNA sub-libraries (40 96-well plates)
  • Human Phosphatase shRNA sub-libraries (15 96-well plates)
  • Human E3 ligase (BTB) shRNA sub-library (9 96-well plates)
  • Human E3 ligase (Ring) shRNA sub-library (15 96-well plates)
  • Human E3 ligase (DDB1-like U box F box SOCS box HECT ZNF_A20) shRNA sub-library (8 96-well plates)


  • Individual Clone distribution
  • Customized shRNA or ORF libraries
  • DNA purification: Facilitated by the Tecan automated workstation, the core will purify DNA in a high throughput manner.
  • Lentiviral production and infection

**The shRNA and ORF reagents are available to MDACC researchers ONLY due to licensing agreements. If you have any questions, please contact Dr. Yutong Sun at



Mien-Chie Hung, Ph.D.
Vice President for Basic Research
Professor and Chair
Department of Molecular and Cellular Oncology- Unit 108
Professor, Department of Surgical Oncology
Distinguished Teaching Professor
Ruth Legett Jones Distinguished Chair
Tel: 713-792-3668
Fax: 713-794-3270


Dihua Yu, M.D., Ph.D.
Hubert L. & Olive Stringer Distinguished Chair in Basic Science
Professor and Deputy Chair
Department of Molecular and Cellular Oncology
Co-Director, Center of Biological Pathways
Tel: 713-792-3636
Fax: 713-792-4454

Yutong Sun, Ph.D.
Assistant professor
Tel: 713-792-3677
Fax: 713-794-3270

Research Scientist:

Longfei Huo, Ph.D.
Tel: 713-795-0524
Fax: 713-794-3270


The shRNA and ORFeome Core offers a few cutting-edge tools tailored for high-throughput screens. To schedule the equipment usage, please contact the core facility directly at,  Dr. Yutong Sun at, or Dr Longfei Huo at

An automated workstation EVO100 (Tecan) is located in a laminar flow hood, which can facilitate high throughput tissue culture.





An automated workstation EVO200 (Tecan) is equipped with both span8 and MCA96 liquid handling arms, a vacuum and an orbital shaker, which allows automated liquid handling, plasmid purification and manipulation in a high throughput manner.




Biotek synergy H4 plate reader is capable of performing absorbance, fluorescence and luminescence assays.






High-Content Imaging Systems:

ImageXpressMicro (Molecular Devices) is a widefield high content microscope capable of providing automated cellular imaging in fluorescent, transmitted light, and phase-contrast modes for fixed- or live-cell assays. It is also equipped with Environmental Control that allows for time-lapse imaging of live cells.



ImageXpressUltra is an automated point-scanning confocal microscope.
Both ImageXpressMicro and ImageXpressUltra  are able to perform automated acquisition and analysis of imagines in multi-well plates (up to 384-well plates).



The high content imaging systems enable investigators to perform image-based high throughput screens, measuring critical events including apoptosis, cell cycle, micronuclei, neurite outgrowth, angiogenesis, intracellular translocation, endocytosis, cell signaling, and multi-wavelength cell scoring.