Confocal Microscopy Core

The Confocal Microscopy Core provides Science Park investigators with the ability to acquire high-resolution multispectral images of cells and tissues using a Zeiss LSM880 with Airyscan confocal microscope. The principal objective of the core is to provide researchers with training and technical support in instrument operation and data analysis using Bitplane's Imaris software package. The core also has an older Zeiss instrument that has been adapted for use as a wide-field epifluoresecence microscope with DIC/Nomarski optics that is also capable of taking time-lapse images.

Services

  • Image acquisition
  • Consultation on new assay development 
  • Training on the principles and applications of confocal microscopy
  • Training and technical support in instrument operation

Applications

  • Analyses of cellular and subcellular distribution of proteins of interest
  • Colocalization studies
  • Analyses of tissue architecture
  • UV laser allowing DNA damage experiments
  • FRET and FRAP
  • Live cell imaging

Director

Collene Jeter, PhD
Assistant Professor, Epigenetics and Molecular Carcinogenesis
Phone: (512) 237-6491
Email: cjeter@mdanderson.org

Image Gallery

 Breast cancer cells showing tumor suppressor-mediated subcellular redistribution of a tumor-promoting oncogene.
NANOG (green) and FOXA1 (red) transcription factors in LNCaP prostate cancer cells cultured in the absence of androgen.
Transgenic mouse cryosection showing aberrant thymic differentiation. 
 Mouse zygote showing differential maternal and paternal chromosomal modifications.