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Hi. I'm Jan Parker-Thornberg, and I'd like to introduce you to some valuable resources that are available to you as a research scientist at M. D. Anderson Cancer Center. Very often research investigators find, that in order to carry out an experiment, its necessary to use a technique or process that they don't have in their lab. This technique may be too expensive to obtain from an outside source or it may not be feasible for an individual lab to buy and maintain special equipment. Or perhaps it maybe that a lab needs a high level of technical support. In fact today, many of the things researchers want to do would be prohibitively expensive or technically impractical if they had to be done by individual labs. That's where Shared Resources come in. These are labs and other resources that provide technical services to all investigators at M. D. Anderson. We have shared resources that can perform all kinds of analyses for you. They use and maintain state of the art equipment and the procedures are performed by experts who specialize in these techniques. So the results will be more uniform and reliable than if they had to be learned by someone in one lab for only a few studies or experiments. We also have labs that provide special imaging techniques that may be critical for an experiment but much too expensive for an individual lab to try to obtain. Investigators can obtain the basic needs for their experiment from shared resources as well. Whether it's a basic or special kind of medium, a certain mouse genotype or special tissues. Several of the shared resources directly support animal experiments, providing or even generating the animals, protocols for their safety and ethical care as well as special techniques for imaging and tumor analysis in small animals.

Likewise for clinical trials, there are shared resources that assist with clinical protocols, protocol data, oversight, and safety monitoring, as well as support for complex clinical studies that require additional nursing and other patient care support or sophisticated laboratory services such as pharmaco kinetic studies or monitoring of neon [phonetic] factors. Not all of the shared resources are labs per se. For example you can consult with bio statisticians who can help with study design, data management and statistical analysis. Or perhaps just study involves that requires a different kind of measurement or analysis, quality of life for example. Lets say you'd like to manage data from family or extended family or from minority populations. Those kinds of expertise are available as well. By using shared resources, investigators can design studies that are not limited by what their lab alone can do. In total M. D. Anderson has over two dozen shared resource facilities, supported by the Cancer Center Support grant or CCSG. You can find links to all of them on the inside M. D. Anderson website. I encourage you to learn more about our shared resources and how they might help ease, enrich or enhance your research.

Hi. I'm Erica Thompson. Welcome to the DNA analysis facility. One of M. D. Anderson's shared resource facilities. The goal of this facility is to provide investigators with access to state of the art instrumentation, technology and expertise in nucleic acid analysis. All of our services are performed by experienced molecular biologists, allowing us to provide investigators with high quality reliable results. This facility opened in 1992 as a sequencing service. Over the past 13 years our services have expanded and now include traditional DNA sequencing, medical re-sequencing, fluorescent fragment analysis, high thrupid [phonetic] plasma isolation, mutation screening using DNA tree HPLC and mouse genotyping by real time PCR. In addition we are developing a sniplex high thruplate [phonetic] snipe detection service and a quantitative GN expression analysis service. DNA sequencing and medical re-sequencing are performed on a 3730XL genetic analyzer. This 96 capillary instrument is capable of sequencing 96 samples in two and half hours. Once loaded this instrument can run for 24 hours unattended. Here you see lead sequence of vigu fogies [phonetic] as she loads a sample plate on to the instrument. Our medical re-sequencing service provides a fast reliable method of detecting variants in cancer related genes. It allows an entire gene to be evaluated in a single experiment. Assays are currently available for the P53, Bracha 1 and Bracha 2 genes. However kits are available for more than 2000 genes. 719 of which are directly related to oncogenesis and tumor suppression. Here our re-sequencing expert TJ uses a thick stake software to assemble genes and identify variants. We now move to the 3100 genetic analyzer. First 16 capillary instrument is used in fluorescent fragment analysis applications. It allows researchers to investigate loss of heterozygosity, and microsatellite instability. Here we see Sandy Sen [inaudible] as she reviews [inaudible] generated by this instrument. Real time PCR is used to genotype transgenic, knock in and knock out mice. It is also used for medium to low thrupid snip determination. This facility has four real time PCR instruments and currently provides assays for more than 15 genes. We also have a custom assay design in optimization service. Finally we move to our snip hunting service. The transgenomic wave uses genetry HPO technology to rapidly and cost effectively screen PCR products for polymorphisms and mutations. Here we see DJ, as she prepares to load samples for injection into the HPO secaller [phonetic]. Once detected, mutations must be confirmed by sequencing. Thanks for listening our live. If you'd like more information on our facility and an up-to-date list of our services, please our website.

Welcome to the small animal cancer research facility. One of M. D. Anderson's Shared Research Resources. I'm John Hazel. In this facility we maintain a broad range of state of the art technologies for in vivo imaging in small animals. Our faculty and staff research consult with cancer researchers to help them identify the best imaging modalities to solve cancer related biological problems. Through our own research and in collaboration with others we also develop and use novel and experimental imaging techniques such as gated micro CT, multi-mouse MRI, dynamic contrast MRI, and multi-animal nuclear imaging. Our research also includes the development of refined and sensitive contrast materials. Our focus is to develop and use imaging strategies that show physiological advance related tumor growth and physiology, enabling researchers to study metastasis, and other related processes and to assess tumor responsive therapy. For example we are using techniques to image micro MRI profusion to assist investigators to understand angio-genesis and to monitor the spots of anti-angiogenic therapies for several tumor types. We have experts in imaging physics, image processing chemistry and engineering. We work with investigators to develop and implement special imaging equipment or techniques for each particular study. Our animal support team will prepare animals for imaging studies, manage anesthesia during the imaging procedure itself, and they are also available to assist with tissue harvest and collection. Thanks for visiting our facility. We are dedicated to finding ways to use imaging to help investigators understand fundamental cancer biology and develop new therapeutics.

Hi. I'm Jaclyn Furr and this is the histopathology research lab. One of M. D. Anderson's shared resource facilities. We have two labs, one on the south campus and one in the hospital complex where we assist M. D. Anderson researchers whose projects require histologic analysis. We do all routine histological procedures. But also special stains, frozen sectioning, laser capture micro dissectioning, DNA RNA controlled sectioning and imunohistochemical sectioning and staining. We work with the investigators to determine what the needs of their study are. And we're constantly adding to the services we can offer. Let me show you some of our work. Here you see Neon, performing a routine procedure of cutting paraffin sections of tumor for hemotoxin and ESN or HNE staining. In the core lab, sections are routinely cut at four microns and all unstained slides are put on positively charged slides. Here we see Lorenza, doing a special stain, a gram stain to identify bacteria in mouse tissue for one of our investigators. Here Sharita is cutting frozen sections for laser capture micro-detection which requires RNAs controlled conditions. We have Lorenza here again, demonstrating the proper procedure for investigators to label cassettes that are brought to the lab. Placing your order number on the cassette is a very important factor in processing your order. Here Sharita is doing dilutions for CT20 immunostain. This is to identify B cell mockers in tissue cells. We do IHC staining on animal research tissue only. And here you see Sicily using the online database system that we have to help users create, edit and track their orders. Thanks for visiting our lab. To learn more about our facility or to get started using our services, please call us or visit our website.

Welcome to the genetically engineered mouse facility. I'm Jan Parker-Thornberg. This lab is one of M. D. Anderson's shared resources. In our facilities we can consult with you to design your mouse project, create the gene knock out or transgenic mouse for you and then once you're finished with the project, archive the animals you no longer need by kryo preserving embryos. Let me show you some of our work. Here you see Marto Rohas and Suhe Lopez working on ESL projects to make cell clones that a contain a defined mutation. In this procedure the DNA is generated by an individual investigator. Our role is to get that DNA into the cells. We can use several different types of ES cells for projects, depending on what best suites the investigator's needs. In this lab we perform per nuclear injections to make transgenic mice, as well as blast assist injections to make knock out and knock in mice. Jennifer Wanna is our blast assist injectionist. Here you can see Jennifer injecting ES cells into an extended blast. Chad Smith is in the process of freezing embryos. Once a project is winding down its more cost efficient to freeze a line rather than continue breeding it. Kryo preservation of animals can also guard against the loss of a valuable line or prevent genetic drift. Thanks for visiting our lab. If you'd like more information about our facility, let me encourage you to visit our website. You can link to it from the shared resources page on the Inside M. D. Anderson website.