Skip to Content

IVIS Spectrum

Instrument

IVIS SpectrumThe IVIS Spectrum uses optical imaging technology to facilitate non-invasive longitudinal monitoring of disease progression, cell trafficking and gene expression patterns in living animals.

The IVIS Spectrum is the most versatile and advanced in vivo imaging system. An optimized set of high efficiency filters and spectral un-mixing algorithms lets you take full advantage of bioluminescent and fluorescent reporters across the blue to near infrared wavelength region. It also offers single-view 3D tomography for both fluorescent and bioluminescent reporters that can be analyzed in an anatomical context using Digital Mouse Atlas.

For advanced fluorescence imaging, the IVIS Spectrum has the capability to use either trans-illumination (from the bottom) or epi-illumination (from the top) to illuminate in vivo fluorescent sources. 3D diffuse fluorescence tomography can be performed to determine source localization and concentration using the combination of structured light and trans illumination fluorescent images.

The instrument is equipped with 10 narrow band excitation filters (30nm bandwidth) and 18 narrow band emission filters (20nm bandwidth) that assist in significantly reducing auto-fluorescence by the spectral scanning of filters and the use of spectral un-mixing algorithms. In addition, the spectral un-mixing tools allow the researcher to separate signals from multiple fluorescent reporters within the same animal.

Applications

The IVIS Spectrum can be used in oncology research to follow disease progression, to detect micro metastasis and metastasis spontaneously generated from primary tumors, to accurately quantify tumor burden, and effectively monitor responses to therapeutic treatments longitudinally. 

Other uses include: infectious disease, inflammation studies, metabolic disease, neurology, gene therapy, stem cell biology, cardiovascular disease, immunology, transplantation biology, and drug metabolism.

Monitoring Tumor Progression and Drug Response

↓ Pancreatic tumor growth in primary site and metastasis to peritoneal cavity and liver. Firefly luciferase labeled AsPc-1- pancreatic tumor cells were used.

Cancer Detection

↓ Early detection of pancreatic cancer by using cancer specific Cy5.labeled probe.


© 2014 The University of Texas MD Anderson Cancer Center