Tutorials
Introduction to Biological Mass Spectrometry
Electrospray Ionization (ESI)
Matrix-Assisted Laser Desorption Ionization (MALDI)
Time-of-Flight (TOF) Mass Analysis
Introduction to Mass Spectrometry Measurements for Proteomics
- Measurement of Protein Molecular Weight using MALDI MS or ESI MS
- Verify known protein
- Determine type and amount of post-translational modification to known protein
- First pass analysis for unknown protein
- Peptide Mass Fingerprinting (PMF) using Proteolytic Digestion and Accurate Mass Measurement
- Verify known protein by matching to database entries
- Identify unknown proteins by matching to database entries
- Localize post-translational modifications to individual peptides
- Peptide Sequencing by MS/MS or Enzymatic Digestion Coupled to MS
- Identify unknown proteins using partial sequence and database matching
- Localize post-translational modifications to individual amino acids
- Verify known proteins
Other Related Links
The Nobel Prize in Chemistry 2002