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Services

PROTEOMICS

Protein Identification

High sensitivity (silver stain OK), up to 100 proteins or more may be identifiable.

Cost: $200/sample

Turnaround Time: 5-10 working days

Molecular Weight Determination

Check the MW of your peptide or molecule. Proteins may also be measured if enough clean protein is available  (larger proteins are less reliable)

Cost: $50/sample

Turnaround Time: 4-10 working days

Quantitative Protein Analysis

Using stable isotope methods, iTRAQ, etc. Please inquire.
Using non-isotopic methods. 

  • For SRM/MRM methods on our tandem quadrupole MS please inquire.

Posttranslational Modification Analysis

Phosphorylation and acetylation. Please inquire for more detailed information and other analyses that can be performed. 

Cost: $500/sample

Turnaround Time: Will Vary

HPLC-MS

Size exclusion chemotography and simple LC-MS. Please inquire for more detailed information and other analyses that can be performed.

Cost: $100/sample

Turnaround Time: Will vary

Edman Degradation

Please inquire

Quantitative Metabolite Analysis

Please inquire

 

NEW!! Large-scale PhosphoProteomics! - Please Inquire!!

 

The brand new Orbitrap Fusion has been installed and is performing well!  

**We gratefully acknowledge the NIH High-End Instrumentation program grant# 1S10OD012304-01 for funding our acquisition of the newest Orbitrap technology available today.

 

Do you need higher resolution than you see here or some other MS service?

Please inquire - we are happy to consult with you and help arrange the MS resource you require

METABOLOMICS 

Please email or call for an appointment to discuss your project!

 

 How to Submit Samples for Processing

1. Email David Hawke at dhawke@mdanderson.org to set an appointment to discuss the details of your experimental design.

2. Go to mdanderson.ilabsolutions.com and set up your online account. Once that is complete, you can request services here.

3. Upon approval of your online submission, you will be notified via email to set an appointment for sample drop off. Note that samples will not be accepted without an appointment!

Other Necessary Forms

Credit Card Authorization Form (pdf) 

Bio-Specimen Transportation Form (MDACC Employee Shuttle Only)

Shuttle Form (pdf)

Payment Methods

INSIDE MDACC:

PeopleSoft Account Number

OUTSIDE MDACC:

Credit Card or Purchase Order (PO)

 

Silver Staining of Protein Gels

Recommended Silver Stain

Download Silver Stain Protocol (pdf).  Please ignore destain, we destain after cutting the bands

Reference:  Morrissey, J.H. (1981).Silver stain for proteins in polyacrylamide gels: A modified procedure with enhanced uniform sensitivity. Anal. Biochem. 117: 307-310.

Reagents

Silver Solution

  • 0.1% AgNO3 in H2O: 0.1g AgNO3 dissolved in 100 ml H2O

Developing Solution

  • 3% Sodium carbonate (Na2CO3) in H2O
  • 50 µl formaldehyde/100 ml
  • Stir for 2 minutes

Procedure

All incubations are at room temperature, and carried out with gentle shaking on a shaking platform. All steps take place in a clean (acid washed) pyrex dish. 

Make up all solutions fresh, just before use.

  • Incubate 15 min in 50% methanol
  • Incubate 15 min in 32 µM DTT (8 µl 1M DTT /250 ml water) solution (freshly made)
  • Wash briefly (approximately 5 to 10 seconds) with H2O, twice
  • Wash with a little Silver Solution, pour away, then add the rest of the solution and incubate for 15 minutes
  • Wash briefly (approximately 5 to 10 seconds) with H2O, twice
  • Twice wash with a little Developing Solution, pour away, then add the rest of the solution and incubate until the bands are the desired intensity
  • Pour off most of the developing solution, sprinkle solid citric acid into the solution containing the gel and swirl. Continue slowly adding the citric acid until the fizzing ceases
  • Add a little H2O, and incubate 15 minutes
  • Incubate 3 X 15 minutes with 3 changes of H2O
  • Preferred: Bring the gel to our lab; we prefer to cut the bands here

 

Results from Proteomics Tracker

Now available on the WWW at https://biostatistics.mdanderson.org/ProteomicsTracker  !!!


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