Price Structure*
Monoclonal Antibody Facility Services
| Name of Service | Unit of Measure | Current Charge |
| Immunization and fusion | Antigen | $1,500 |
| Screening and positive selection | Fusion procedure | $1,500 |
| 1st cloning and characterization | Fusion procedure | $1,500 |
| Recloning/subcloning | Clone | $250 |
| Small-scale antibody purification (Protein A) | Clone up to 100 ml | $200 |
| Small-scale antibody purification (Protein A) | Clone 100 ml to 250 ml | $240 |
| Small-scale antibody purification (Protein A) | Clone 250 ml to 500 ml | $300 |
| Small-scale antibody purification (Protein A) | Clone 500 ml to 1 liter | $350 |
| Ascites fluid production | Mouse | $250 |
| Ascites fluid production/ nude mice | Mouse | $300 |
| In vitro supernatant production (TC) | Up to liter | $350 |
| In vitro supernatant production (TC) | Up to 100 ml | $250 |
| Antibody purification (FPLC) | Sample | $350 |
| Antibody conjugation | Sample | $280 |
| F(ab) and F(ab)2 fragment purification | Sample | $400 |
| Endotoxin test ($150 kit) | Antibody | $50 |
| Supernatant concentration | Antibody up to 500 ml | $80 |
| Sample preparation for IHC Core (cell expansion, pellet, fixation) | Cell line | $300 |
| Clone restock | Cell line hybridoma | $50 |
| Other extra services (PAGE, ELISA, etc.) | Antibody | $150 |
| FACS staining | Up to 20 clones | $150 |
| Mycoplasma test | Cell line | $50 |
Overview of Hybridoma Development
| Footpad Injection |
| Day 1-18 | Immunization every three days up to day 18 |
| Day 14 | Anti-serum antibody titration |
| Subcutaneous Injection |
| Day 1-40 | Immunization every 14 days up to day 40 |
| Day 30-34 | Anti-serum antibody titration |
| Fusion |
| Day 1-10 | Fusion and screening |
| Day 14-15 | Supernatant antibody available for antibody assays and WBs |
| Subcloning |
| Day 1-10 | Subcloning by limiting dilution and screening |
| Purification |
| Supernatant |
| Day 1-10 | In vitro culture supernatant production and purification |
| Ascites |
| Day 1-10 | In vivo ascites production and purification |
* All fees stated are for "in-house" investigators. PIs from outside institutions should contact the Monoclonal Antibody Facility manager for more information.
