Price Structure*
Monoclonal Antibody Development
Immunization and Fusion: | $1,500 |
| Screening and Positive Selection: | $1,500 |
| 1st cloning and characterization: | $1,500 |
Additional Charges
2nd cloning and small-scale purification | $125/clone/100ml |
| Recloning/Subcloning | $125/clone |
| Ascites Fluid Production | $125/mouse |
| Ascites Production Nude mice | $200/mouse |
| In vitro Supernatant Production | $250/1 liter |
| Antibody Purification (Protein G) | $250 |
| Antibody Purification (FPLC) | $350 |
| Antibody Conjugation | $250 |
| F(ab) and F(ab)2 fragment purification | $350 |
Overview of Hybridoma Development
| Immunization | |
| Footpad Injection | |
| Day 1-18 | Immunization every three days up to day 18 |
| Day 14 | Anti-serum antibody titration |
| Subcutaneous Injection | |
| Day 1-40 | Immunization every 14 days up to day 40 |
| Day 30-34 | Anti-serum antibody titration |
| Fusion | |
| Day 1-10 | Fusion and screening |
| Day 14-15 | Supernatant antibody available for antibody assays and WBs |
| Subcloning | |
| Day 1-10 | Subcloning by limiting dilution and screening |
| Purification | |
| Supernatant | |
| Day 1-10 | In vitro culture supernatant production and purification |
| Ascites | |
| Day 1-10 | In vivo ascites production and purification |
* All fees stated are for "in-house" investigators. PIs from outside institutions should contact the Monoclonal Antibody Facility manager for more information.