TP53 Mutation Analysis
Indication
To detect and type mutations in the DNA-binding domain of the TP53 tumor suppressor gene for diagnosis of Li-Fraumeni syndrome and for prognosis and therapy selection of a wide range of cancer types.
Methodology
PCR-based DNA sequencing; this assay also available on Next Gen Sequencing panels.
Test Parameters
This assay will detect mutations in exons 4 to 9. The sensitivity of the assay is 20% of mutant sequence in the background of wild type sequence.
Turnaround Time
10 days
Sample Requirements
10 mL peripheral blood, 2-5mL bone marrow aspirate, 5mg purified DNA, or 4-10 unstained tissue sections on glass slides along with 1 H&E-stained section.
81405