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TP53 Mutation Analysis

Indication

To detect and type mutations in the DNA-binding domain of the TP53 tumor suppressor gene for diagnosis of Li-Fraumeni syndrome and for prognosis and therapy selection of a wide range of cancer types.

Methodology

PCR-based DNA sequencing; this assay also available on Next Gen Sequencing panels.

Test Parameters

This assay will detect mutations in exons 4 to 9. The sensitivity of the assay is 20% of mutant sequence in the background of wild type sequence.

Turnaround Time

10 days

Sample Requirements

10 mL peripheral blood, 2-5mL bone marrow aspirate, 5mg purified DNA, or 4-10 unstained tissue sections on glass slides along with 1 H&E-stained section.

81405


© 2014 The University of Texas MD Anderson Cancer Center