T-cell Receptor Beta (TCRB) Gene Rearrangement
– Assessment of clonality in suspected T-cell lymphoproliferations and lymphoproliferative neoplasm of uncertain lineage
– As a marker of neoplastic cells in a subset of B- and T-lymphoblastic leukemias
A semi-automated PCR method involving fluorescent-labeled PCR products and non-denaturing capillary electrophoresis is performed to detect only complete TCRB rearrangements.
The sensitivity of this assay for detection of a clonal T-cell population is between 1:100 and 1:1,000 lymphocytes, depending on the number of polyclonal T-cells present. T-cell tumor expressing the gamma-delta T-cell receptor will not be detected. Clonal TCRB rearrangements can be seen in B-cell acute lymphoblastic leukemias and are thus not definitive for lineage in immature lymphoid neoplasms.
Five to seven working days
- 10 ml peripheral blood in purple top tube (EDTA Vacutainer), sent on wet ice
- 2-5 ml of bone marrow aspirate, sent on wet ice
- Frozen tissue samples
- Four to six unstained recut slides/shaves from formalin-fixed, paraffin-embedded tissue
- 10 ug of purified DNA, sent on dry ice
Please provide a copy of the corresponding pathology report.
Additional charges will apply for tissue extraction.
The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.