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T-cell Receptor Beta (TCRB) Gene Rearrangement

PCR

Indication

– Assessment of clonality in suspected T-cell lymphoproliferations and lymphoproliferative neoplasm of uncertain lineage
– As a marker of neoplastic cells in a subset of B- and T-lymphoblastic leukemias

Methodology

A semi-automated PCR method involving fluorescent-labeled PCR products and non-denaturing capillary electrophoresis is performed to detect only complete TCRB rearrangements.

Test Parameters

The sensitivity of this assay for detection of a clonal T-cell population is between 1:100 and 1:1,000 lymphocytes, depending on the number of polyclonal T-cells present. T-cell tumor expressing the gamma-delta T-cell receptor will not be detected. Clonal TCRB rearrangements can be seen in B-cell acute lymphoblastic leukemias and are thus not definitive for lineage in immature lymphoid neoplasms.

Turnaround Time

Five to seven working days

Sample Requirements

  • 10 ml peripheral blood in purple top tube (EDTA Vacutainer), sent on wet ice

    or
  • 2-5 ml of bone marrow aspirate, sent on wet ice

    or
  • Frozen tissue samples

    or
  • Four to six unstained recut slides/shaves from formalin-fixed, paraffin-embedded tissue

    or
  • 10 ug of purified DNA, sent on dry ice

Please provide a copy of the corresponding pathology report.

CPT Codes

81340

Additional charges will apply for tissue extraction.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.


© 2014 The University of Texas MD Anderson Cancer Center