T-cell Receptor Beta Gene Rearrangement
PCR
Indication
The detection of a clonal T-cell rearrangement in lymphoproliferative disorders, as an aid to diagnosis, lineage assignment or as a followup for residual disease.
Methodology
Fluorescence-based polymerase chain reaction (PCR) assay to assess for T-cell receptor beta chain gene rearrangements.
Test Parameters
The sensitivity of this assay for detection of a clonal T-cell population is between 1:100 and 1:1,000 lymphocytes, depending on the number of polyclonal T-cells present. T-cell tumor expressing the gamma-delta T-cell receptor will not be detected. Clonal TCR-beta rearrangements can be seen in B-cell acute lymphoblastic leukemias and are thus not definitive for lineage in immature lymphoid neoplasms.
Turnaround Time
Five to seven working days
Sample Requirements
- 10 ml peripheral blood in purple top tube (EDTA Vacutainer), sent on wet ice
or - 2-5 ml of bone marrow aspirate, sent on wet ice
or - Frozen tissue samples
or - Four to six unstained recut slides/shaves from formalin-fixed, paraffin-embedded tissue
or - 10 ug of purified DNA, sent on dry ice
Please provide a copy of the corresponding pathology report.
CPT Codes
83890 (DNA Isolation), 83900, 83909, 83912
Additional charges will apply for tissue extraction.
The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.