PTEN Mutation Analysis
To detect and type mutations in the PTEN tumor suppressor gene for diagnosis of Cowden disease and Bannayan-Riley-Rucvalcaba syndrome and for prognosis and therapy selection in range of cancer types, particularly endometrial carcinoma, glioblastoma multiforme, melanoma, and prostatic carcinoma.
This assay is part of the Next Gen Sequencing panel for solid tumors.
Next generation sequencing-based analysis is performed to detect point mutations in mutational hotspots in the PTEN gene. The codons tested include, 6, 68, 173, 171, 214, 242, 248, 267, 290, 317, 318, 319, 321, 323, 335.
5ug purified DNA or 4-10 unstained tissue sections on glass slides along with 1 H&E-stained section.