NPM1 Mutational Analysis
PCR
Indication
Mutations in the form of nucleotide insertions in exon 12 of the nucleophosmin (NPM1) gene are found in 25-35% of all cases of acute myeloid leukemia (AML) and 45-60% of cases of karyotypically normal AML. AML with mutant NPM1 and wild-type FLT3 is associated with a favorable prognosis.
Methodology
PCR-based amplification followed by amplicon sizing using Genescan.
Test Parameters
This assay will detect mutations in exon 12 of the NPM1 gene. The sensitivity of detection is approximately 2.5%.
Turnaround Time
Four to six working days
Sample Requirements
- 10 ml peripheral blood in purple top tube (EDTA Vacutainer), sent on wet ice
or - 2-5 ml of bone marrow aspirate, sent on wet ice
CPT Codes
83890 (DNA Isolation), 83898, 83909, 83912
The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.